公开(公告)号：US20140227723A1

公开(公告)日：2014-08-14

申请号：US14233553

申请日：2012-07-18

Applicant: Donald E. Ingber ,  Michael Super ,  Jeffrey Charles Way ,  Mark J. Cartwright ,  Julia B. Berthet ,  Dinah R. Super ,  Martin M. Rottman ,  Alexander Watters

Inventor： Donald E. Ingber ,  Michael Super ,  Jeffrey Charles Way ,  Mark J. Cartwright ,  Julia B. Berthet ,  Dinah R. Super ,  Martin M. Rottman ,  Alexander Watters

IPC: C07K16/12 ,  G01N33/569 ,  C07K14/42

CPC classification number: C07K16/12 ,  A61K47/68 ,  A61K47/6815 ,  C07K14/42 ,  C07K2319/33 ,  G01N33/54353 ,  G01N33/56911 ,  G01N33/56916 ,  G01N33/56938 ,  G01N33/56961 ,  G01N2333/42 ,  G01N2333/4724 ,  Y02A50/53 ,  Y02A50/58

Abstract: Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.

Abstract translation: 本文描述了工程化的微生物靶向或微生物结合分子，包含其的试剂盒及其用途。 微生物靶向或微生物结合分子的一些具体实施方案包含与Fc区的一部分连接的甘露糖结合凝集素或其片段的碳水化合物识别结构域。 在一些实施方案中，微生物靶向分子或微生物结合分子可以与基底（例如磁性微珠）缀合，形成微生物靶向底物（例如，微生物靶向磁性微珠）。 这样的微生物靶向分子和/或底物及其包含的试剂盒可以结合和/或捕获微生物和/或其微生物物质，因此可以用于各种应用中，例如诊断和/或治疗感染 由微生物引起，如受试者或任何环境表面的败血症。 微生物靶向分子和/或底物可以在使用后通过用不溶于钙的低pH缓冲液或缓冲液洗涤而再生。

公开(公告)号：US09593160B2

公开(公告)日：2017-03-14

申请号：US14233553

申请日：2012-07-18

Applicant: Donald E. Ingber ,  Michael Super ,  Jeffrey Charles Way ,  Mark J. Cartwright ,  Julia B. Berthet ,  Dinah R. Super ,  Martin M. Rottman ,  Alexander Watters

Inventor： Donald E. Ingber ,  Michael Super ,  Jeffrey Charles Way ,  Mark J. Cartwright ,  Julia B. Berthet ,  Dinah R. Super ,  Martin M. Rottman ,  Alexander Watters

IPC: C07K14/42 ,  C07K16/12 ,  A61K47/48 ,  G01N33/543 ,  G01N33/569

CPC classification number: C07K16/12 ,  A61K47/68 ,  A61K47/6815 ,  C07K14/42 ,  C07K2319/33 ,  G01N33/54353 ,  G01N33/56911 ,  G01N33/56916 ,  G01N33/56938 ,  G01N33/56961 ,  G01N2333/42 ,  G01N2333/4724 ,  Y02A50/53 ,  Y02A50/58

Abstract: Described herein are engineered microbe-targeting or microbe-binding molecules, kits comprising the same and uses thereof. Some particular embodiments of the microbe-targeting or microbe-binding molecules comprise a carbohydrate recognition domain of mannose-binding lectin, or a fragment thereof, linked to a portion of a Fc region. In some embodiments, the microbe-targeting molecules or microbe-binding molecules can be conjugated to a substrate, e.g., a magnetic microbead, forming a microbe-targeting substrate (e.g., a microbe-targeting magnetic microbead). Such microbe-targeting molecules and/or substrates and the kits comprising the same can bind and/or capture of a microbe and/or microbial matter thereof, and can thus be used in various applications, e.g., diagnosis and/or treatment of an infection caused by microbes such as sepsis in a subject or any environmental surface. Microbe-targeting molecules and/or substrates can be regenerated after use by washing with a low pH buffer or buffer in which calcium is insoluble.

Abstract translation: 本文描述了工程化的微生物靶向或微生物结合分子，包含其的试剂盒及其用途。 微生物靶向或微生物结合分子的一些具体实施方案包括与Fc区的一部分连接的甘露糖结合凝集素或其片段的碳水化合物识别结构域。 在一些实施方案中，微生物靶向分子或微生物结合分子可以与基底（例如磁性微珠）缀合，形成微生物靶向底物（例如，微生物靶向磁性微珠）。 这样的微生物靶向分子和/或底物及其包含的试剂盒可以结合和/或捕获微生物和/或其微生物物质，因此可用于各种应用中，例如诊断和/或治疗感染 由微生物引起，如受试者或任何环境表面的败血症。 微生物靶向分子和/或底物可以在使用后通过用不溶于钙的低pH缓冲液或缓冲液洗涤而再生。

公开(公告)号：US20160311877A1

公开(公告)日：2016-10-27

申请号：US15105298

申请日：2014-12-18

Applicant: Alexander WATTERS ,  Donald INGBER ,  Mark CARTWRIGHT ,  Michael SUPER ,  Martin ROTTMAN ,  PRESIDENT AND FELLOWS OF HARVARD COLLEGE

Inventor： Alexander WATTERS ,  Donald INGBER ,  Mark CARTWRIGHT ,  Michael SUPER ,  Martin ROTTMAN ,  Evangelina MURRAY ,  Brendon DUSEL

IPC: C07K14/47 ,  A61K47/42 ,  G01N33/569

Abstract: Described herein are engineered microbe-targeting molecules, microbe-targeting articles, kits comprising the same, and uses thereof. Such microbe-targeting molecules, microbe-targeting articles, or the kits comprising the same can bind or capture of a microbe or microbial matter thereof, and can thus be used in various applications, such as diagnosis or treatment of an infection caused by microbes in a subject or any environmental surface.

Abstract translation: 本文描述了工程化的微生物靶向分子，微生物靶向制品，包含其的试剂盒及其用途。 这样的微生物靶向分子，微生物靶向制品或包含其的试剂盒可以结合或捕获其微生物或微生物物质，因此可以用于各种应用中，例如诊断或治疗由微生物引起的感染 主题或任何环境表面。