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    DUAL COFACTOR SPECIFIC RIBITOL DEHYDROGENASE AND USE THEREOF
    1.
    发明申请
    DUAL COFACTOR SPECIFIC RIBITOL DEHYDROGENASE AND USE THEREOF 有权
    双重特异性RIBITOL DEHYDROGENASE及其用途

    公开(公告)号：US20140242642A1

    公开(公告)日：2014-08-28

    申请号：US13880374

    申请日：2012-01-27

    申请人： Jung Kul Lee ,  Hee Jung Moon ,  Manish Tiwari ,  Tae Su Kim

    发明人： Jung Kul Lee ,  Hee Jung Moon ,  Manish Tiwari ,  Tae Su Kim

    IPC分类号： C12P19/02 ,  C12N9/04

    CPC分类号： C12P19/02 ,  C12N9/0006 ,  C12Y101/01056

    摘要： There are provided a novel ribitol dehydrogenase, a residue determining double coenzyme specificity, and a method for preparing L-ribulose using the same, and more particularly, to a ribitol dehydrogenase producing rare sugars, nucleic acid molecules encoding the same, a vector including the nucleic acid molecules, a transformant including the vector, a mutant of the ribitol dehydrogenase, and a method for preparing L-ribulose using the ribitol dehydrogenase. The ribitol dehydrogenase having double coenzyme specificity, which is derived from Zymomonas mobilis, can effectively be used for preparing high-priced rare sugars and an investigation of coenzyme specificity determinants for the ribitol dehydrogenase is applied for all of dedydrogenases as a based technique.

    摘要翻译： 提供了新的核糖醇脱氢酶，确定双辅酶特异性的残基，以及使用其制备L-核酮糖的方法，更具体地，涉及制备核糖醇脱氢酶的稀有糖，编码它的核酸分子，包含 核酸分子，包含载体的转化体，核糖醇脱氢酶的突变体和使用核糖醇脱氢酶制备L-核酮糖的方法。 具有双辅酶特异性的衍生自运动发酵单胞菌的核糖醇脱氢酶可以有效地用于制备高价稀有糖，并且对所有的脱氢酶作为基础技术应用于核糖醇脱氢酶的辅酶特异性决定簇的研究。

    Fermentation process for preparing coenzyme Q10 by the recombinant Agrobacterium tumefaciens
    4.
    发明申请
    Fermentation process for preparing coenzyme Q10 by the recombinant Agrobacterium tumefaciens 审中-公开
    由重组农杆菌制备辅酶Q10的发酵方法

    公开(公告)号：US20050181490A1

    公开(公告)日：2005-08-18

    申请号：US11042209

    申请日：2005-01-26

    申请人： Soo-Ryun Cheong ,  Sang-Young Kim ,  Jung-Kul Lee ,  Hyeon-Cheol Lee ,  Suk-Jin Ha ,  Bong-Seoung Koo ,  Ji-Hyun Yoo

    发明人： Soo-Ryun Cheong ,  Sang-Young Kim ,  Jung-Kul Lee ,  Hyeon-Cheol Lee ,  Suk-Jin Ha ,  Bong-Seoung Koo ,  Ji-Hyun Yoo

    IPC分类号： C12N15/09 ,  C12N1/21 ,  C12N9/10 ,  C12N9/12 ,  C12N9/88 ,  C12N15/74 ,  C12P7/66 ,  C12Q1/68 ,  C12R1/01

    CPC分类号： C12P7/66 ,  C12N9/1022

    摘要： The present invention relates to a transformed Agrabacterium tumefaciens BNQ-pGPRX11 (Accession No. KCCM-10554) harboring a recombinant expression vector (pGPRX11). Further, the present invention also provides a fermentation method for maximum production of coenzyme Q10 using a transformed Agrabacterium tumefaciens deposited to Korean Culture Center of Microorganism with accession number KCCM-10554 comprising the steps of: i) fermenting transformed cells on production medium comprising 30˜50 g/L of corn steep powder, 0.3˜0.7 g/L of KH2PO4, 0.3˜0.7 g/L of K2HPO4, 12˜18 g/L of ammonium sulfate, 1.5˜2.5 g/L of lactic acid, 0.2˜0.3 g/L of magnesium sulfate on condition that aeration rate of the medium is 0.8˜1.2 volume of air per volume of medium per minute, temperature 30˜34° C. and pH is 6.0˜8.0; ii) removing the transformed cells and other residue from the fermentation medium; and iii) separating and recovering coenzyme Q10 from the fermentation medium of step (ii).

    摘要翻译： 本发明涉及携带重组表达载体（pGPRX11）的转化的根癌农杆菌BNQ-pGPRX11（登录号KCCM-10554）。 此外，本发明还提供了使用保藏于韩国文化中心的保藏号为KCCM-10554的转化的根癌农杆菌来最大限度地生产辅酶Q 10的发酵方法，包括以下步骤：i）发酵 包含30〜50g / L的玉米糠粉，0.3〜0.7g / L的KH 2 PO 3，0.3〜0.7g / L的生产培养基中的转化细胞， K 2 N 2 HPO 4，12〜18g / L硫酸铵，1.5〜2.5g / L乳酸，0.2〜0.3g / L硫酸镁条件 培养基的通气速率为每分钟体积培养基的空气量为0.8〜1.2，温度为30〜34℃，pH为6.0〜8.0; ii）从发酵培养基中去除转化的细胞和其它残留物; 和iii）从步骤（ii）的发酵培养基中分离并回收辅酶Q 10。

    Dual cofactor specific ribitol dehydrogenase and use thereof
    5.
    发明授权
    Dual cofactor specific ribitol dehydrogenase and use thereof 有权
    双辅因子特异性核糖醇脱氢酶及其用途

    公开(公告)号：US09005937B2

    公开(公告)日：2015-04-14

    申请号：US13880374

    申请日：2012-01-27

    申请人： Jung Kul Lee ,  Hee Jung Moon ,  Manish Tiwari ,  Tae Su Kim

    发明人： Jung Kul Lee ,  Hee Jung Moon ,  Manish Tiwari ,  Tae Su Kim

    IPC分类号： C12P19/02 ,  C12N9/00 ,  C12N9/04 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C12P19/02 ,  C12N9/0006 ,  C12Y101/01056

    摘要： There are provided a novel ribitol dehydrogenase, a residue determining double coenzyme specificity, and a method for preparing L-ribulose using the same, and more particularly, to a ribitol dehydrogenase producing rare sugars, nucleic acid molecules encoding the same, a vector including the nucleic acid molecules, a transformant including the vector, a mutant of the ribitol dehydrogenase, and a method for preparing L-ribulose using the ribitol dehydrogenase. The ribitol dehydrogenase having double coenzyme specificity, which is derived from Zymomonas mobilis, can effectively be used for preparing high-priced rare sugars and an investigation of coenzyme specificity determinants for the ribitol dehydrogenase is applied for all of dedydrogenases as a based technique.

    摘要翻译： 提供了新的核糖醇脱氢酶，确定双辅酶特异性的残基，以及使用其制备L-核酮糖的方法，更具体地，涉及制备核糖醇脱氢酶的稀有糖，编码它的核酸分子，包含 核酸分子，包含载体的转化体，核糖醇脱氢酶的突变体和使用核糖醇脱氢酶制备L-核酮糖的方法。 具有双辅酶特异性的衍生自运动发酵单胞菌的核糖醇脱氢酶可以有效地用于制备高价稀有糖，并且对所有的脱氢酶作为基础技术应用于核糖醇脱氢酶的辅酶特异性决定簇的研究。

    Fermentation Process for Preparing Coenzyme Q10 by the Recombinant Agrobacterium tumefaciens
    6.
    发明申请
    Fermentation Process for Preparing Coenzyme Q10 by the Recombinant Agrobacterium tumefaciens 审中-公开
    由重组农杆菌制备辅酶Q10的发酵方法

    公开(公告)号：US20080261282A1

    公开(公告)日：2008-10-23

    申请号：US12044379

    申请日：2008-03-07

    申请人： Soo-Ryun Cheong ,  Sang-Young Kim ,  Jung-Kul Lee ,  Hyeon-Cheol Lee ,  Suk-Jin Ha ,  Bong-Seoung Koo ,  Ji-Hyun Yoo

    发明人： Soo-Ryun Cheong ,  Sang-Young Kim ,  Jung-Kul Lee ,  Hyeon-Cheol Lee ,  Suk-Jin Ha ,  Bong-Seoung Koo ,  Ji-Hyun Yoo

    IPC分类号： C12P7/66

    CPC分类号： C12N9/1022 ,  C12P7/66

    摘要： The present invention relates to a transformed Agrobacterium tumefaciens BNQ-pGPRX11 (Accession No. KCCM-10554) harboring a recombinant expression vector (pGPRX11). Further, the present invention also provides a fermentation method for maximum production of coenzyme Q10 using a transformed Agrobacterium tumefaciens deposited to Korean Culture Center of Microorganism with accession number KCCM-10554 comprising the steps of: i) constructing the recombinant expression vector pGPRX11 containing decaprenyl diphosphate synthase gene and 1-deoxy-D-xylulose 5-phosphate synthase gene (SEQ ID NO: 1); ii) preparing a transformed Agrobacterium tumefaciens (KCCM-10554) by harboring said recombinant expression vector pGPRX11 to the host of Agrobacterium tumefaciens BNQ 0605 (KCCM-10413); iii) growing the transformed cells on growth medium comprising 50 g/L of sucrose, 15 g/L of yeast extract, 15 g/L of peptone and 7.5 g/L of NaCl; iv) fermenting transformed cells on production medium comprising 30˜50 g/L of corn steep powder, 0.3˜0.7 g/L of KH2PO4, 0.3˜0.7 g/L of K2HPO4, 12˜18 g/L of ammonium sulfate, 1.5˜2.5 g/L of lactic acid, 0.2˜0.3 g/L of magnesium sulfate on condition that aeration rate of the medium is 0.8˜1.2 volume of air per volume of medium per minute, temperature is 30˜34°C. and pH is 6.0˜8.0; v) removing the transformed cells and other residue from the fermentation medium; and vi) separating and recovering coenzyme Q10 from the fermentation medium of step (v).

    摘要翻译： 本发明涉及带有重组表达载体（pGPRX11）的转化的根癌农杆菌BNQ-pGPRX11（登录号KCCM-10554）。 此外，本发明还提供了使用保藏于韩国文化中心微生物的转化的根癌土壤杆菌，使用登录号KCCM-10554最大限度地生产辅酶Q 10的发酵方法，包括以下步骤：i）构建 含有十聚丙烯酰二磷酸合成酶基因和1-脱氧-D-木酮糖-5-磷酸合酶基因（SEQ ID NO：1）的重组表达载体pGPRX11; ii）通过将所述重组表达载体pGPRX11携带到根癌农杆菌BNQ 0605（KCCM-10413）的宿主中来制备转化的根癌土壤杆菌（KCCM-10554）; iii）在包含50g / L蔗糖，15g / L酵母提取物，15g / L蛋白胨和7.5g / L NaCl的生长培养基上培养转化细胞; iv）在包含30〜50g / L的玉米糠粉末，0.3〜0.7g / L的KH 2 PO 4，0.3〜0.7g的生产培养基中发酵转化的细胞 / L的HPO 4，12〜18g / L的硫酸铵，1.5〜2.5g / L的乳酸，0.2〜0.3g / L的镁 硫酸盐的条件是介质的通气速率为每分钟体积介质的0.8〜1.2体积空气，温度为30〜34℃。 pH 6.0〜8.0; v）从发酵培养基中去除转化的细胞和其它残留物; 和vi）从步骤（v）的发酵培养基中分离并回收辅酶Q 10。

    Mutant strain of Amycolatopsis orientalis and process for preparing vancomycin hydrochloride
    7.
    发明申请
    Mutant strain of Amycolatopsis orientalis and process for preparing vancomycin hydrochloride 审中-公开
    Amycolatopsis orientalis的突变株及其制备万古霉素盐酸盐的方法

    公开(公告)号：US20080193986A1

    公开(公告)日：2008-08-14

    申请号：US11712494

    申请日：2007-03-01

    申请人： Sang Young Kim ,  Do Sun Kim ,  Hyung Moo Jung ,  Jung Kul Lee

    发明人： Sang Young Kim ,  Do Sun Kim ,  Hyung Moo Jung ,  Jung Kul Lee

    IPC分类号： C12P17/18 ,  C12N1/20

    CPC分类号： C12R1/04 ,  C12N1/20 ,  C12P19/44 ,  C12P19/46

    摘要： The present invention provides a process for preparing vancomycin hydrochloride using a mutant strain of Amycolatopsis orientalis (accession No. KCCM-10836P) comprising the steps of i) mutating and isolating a strain of Amycolatopsis orientalis (accession No. KCCM-10836P) for producing vancomycin from mother strain of Amycolatopsis orientalis (accession No. ATCC-19795) using NTG(N-methyl-N′-nitro-N-nitrosoguanidine) in the selection medium; ii) seed culturing the isolated mutant strain of Amycolatopsis orientalis (accession No. KCCM-10836P); iii) cultivating and fermenting said mutant strain of Amycolatopsis orientalis in the fermentation medium consisting of 12˜18 (w/v) % of dextrin, 2.2˜3.8 (w/v) % of bean powder, 1.9˜2.9 (w/v) % of potato protein, 0.10˜0.14 (w/v) % of sodium chloride and a small amount of minerals; iv) filtering vancomycin using microfilter in the cultivation broth by removing mycelia; v) purifying obtained vancomycin using column system compacted with cation exchange resin, anion exchange resin and absorbent resin; and vi) crystallizing the purified vancomycin with hydrochloric acid.

    摘要翻译： 本发明提供一种使用东方异a螨突变菌株（登录号KCCM-10836P）制备万古霉素盐酸盐的方法，其包括以下步骤：i）突变和分离用于产生万古霉素的侧寄生菌株（登录号KCCM-10836P） 从选择培养基中使用NTG（N-甲基-N'-硝基-N-亚硝基胍）的来自东方的抗菌纲菌株（登录号ATCC-19795） ii）种子培养分离的东方异基因突变菌株（登录号KCCM-10836P）; iii）在由12〜18（w / v）％的糊精，2.2〜3.8（w / v）％的豆粉，1.9〜2.9（w / v）的糊状物组成的发酵培养基中，培养和发酵所述的东方双歧杆菌突变菌株， ％的土豆蛋白，0.10〜0.14（w / v）的氯化钠和少量的矿物质; iv）通过去除菌丝体在培养液中使用微过滤器过滤万古霉素; v）使用用阳离子交换树脂，阴离子交换树脂和吸收树脂压实的柱系统纯化得到的万古霉素; 和vi）用盐酸结晶纯化的万古霉素。