摘要:
Recombinant organisms are provided comprising genes encoding genes encoding glycerol dehydratase, 1,3-propanediol oxidoreductase, a gene encoding vitamin B12 receptor precursor(BtuB), a gene encoding vitamin B12 transport system permease protein(BtuC) and a gene encoding vitamin B12 transport ATP-binding protein (BtuD). The recombinant microorganism is contacted with a carbon substrate and 1,3-propanediol is isolated from the growth media.
摘要:
An industrial and economical method for obtaining a (S)-3-halogeno-1,2-propanediol, which comprises cultivating a microorganism belonging to the genus. Pseudomonas which has an ability to assimilate (R)-3-halogeno-1,2-propanediol and can grow by assimilating (R)-3-halogeno-1,2-propanediol as a single carbon source, in a culture medium containing a racemic 3-halogeno-1,2-propanediol as a substrate, and isolating the (S)-3-halogeno-1,2-propanediol from the culture medium.
摘要:
The present invention relates to improved methods and reagents for the production of 1,3-propanediol. In particular, the present invention provides novel thermophilic organisms and thermostable enzymes cable of catalyzing the fermentation of glycerol to 1,3-propanediol. The present invention also relates to methods of isolating such thermophilic organisms, methods of cloning polynucleotides that encode such enzymes, polynucleotides encoding such enzymes, and methods of using such enzymes and organisms for the production of 1,3-propanediol.
摘要:
A bioengineered synthesis scheme for the production of 1,2,3,4-tetrahydroxybenzene from a carbon source is provided. Methods of producing 1,2,3,4-tetrahydroxybenzene from a carbon source based on the synthesis scheme are also provided. Methods are also provided for converting 1,2,3,4-tetrahydroxybenzene to 1,2,3-trihydroxybenzene by catalytic hydrogenation.
摘要:
The present Invention is to provide a novel strain, Candida magnoliae (KCCM-10252) producing mannitol isolated from the fermentation sludge collected from a xylitol manufacturing company, and further a method for manufacturing mannitol with high yield and high productivity by optimizing various culture conditions and medium composition using the new strain, Candida magnoliae.
摘要:
Xylitol is produced by allowing xylitol dehydrogenase or cells instoduced with a DNA coding for xylitol dehydrogenase, which is a protein of the following (A) or (B) to act on D-xylulose, and collecting produced xylitol: (A) a protein which has the amino acid sequence of SEQ ID NO: 4; (B) a protein which has the amino acid sequence of SEQ ID NO: 4 including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and has xylitol dehydrogenase activity.
摘要翻译:通过使木糖醇脱氢酶或用编码木糖醇脱氢酶的DNA(其是以下(A)或(B)的蛋白质)作用于D-木酮糖并收集产生的木糖醇的DNA产生木糖醇:(A) 具有SEQ ID NO:4的氨基酸序列;(B)具有SEQ ID NO:4的氨基酸序列的蛋白质,包括一个或几个氨基酸的取代,缺失,插入,添加或倒位,并且具有木糖醇 脱氢酶活性。
摘要:
The present invention relates to a process for the production of 1,3-propanediol by the fermentation of a 1,3-propanediol-producing microorganism in a fermentation medium containing glucose, characterized in that the fermentation is carried out without mechanical agitation, with the maintenance of an air retention greater than or equal to 50%, expressed as the volume of gas relative to the total volume of the liquid phase of the fermentation medium, and with the maintenance of a high cell density and a microorganism viability value, determined by a test A, greater than or equal to 95%, preferably of between 95 and 99%, by controlling frothing in the fermentation medium.
摘要:
Xylitol is produced by contacting D-arabitol with a microorganism that belongs to the genus Gluconobacter, Acetobacter, Achromobacter, Agrobacterium, Arthrobacter, Azotobacter, Brevibacterium, Corynebacterium, Erwinia, Flavobacterium, Micrococcus, Nocardia, Planococcus, Pseudomonas, Rhodococcus, Morganella, Actinomadura, Actinomyces, or Streptomyces and is capable of converting D-arabitol to xylitol, and recovering xylitol thus produced.
摘要:
A yeast strain capable of converting glucose to erythritol, said strain having the following identifying characteristics: an absence of motile spores; septate mycelia; asexual reproduction; an absence of reniform cells; conidia optionally formed on short denticles but not on elongate stalks; an absence of ballistoconidia; non-monopolar budding on a broad base; acropetal chains of blastoconidia; dark brown, thick-walled chlamydospores; an ability to assimilate sucrose, glycerol and maltose; an inability to assimilate lactose; an inability to ferment galactose; an ability to grow in a vitamin-free medium; and an ability to grow at 25° C. to 36° C.
摘要:
The present invention relates to a fermentation process to have a high productivity with a novel mutant of Torula sp. DS101 (KCMM-10171), more specifically, for preparing erythritol using mother liquor produced from purification process, which comprises 30˜60% of trehalulose, 10˜30% of palatinose, 5˜15% of fructose, 5˜25% of glucose and 0˜10% of sucrose.