Process for preparing (S)-3-halogeno-1,2-propanediol by microorganism
    2.
    发明授权
    Process for preparing (S)-3-halogeno-1,2-propanediol by microorganism 失效
    用微生物制备(S)-3-卤代-1,2-丙二醇的方法

    公开(公告)号:US06316233B2

    公开(公告)日:2001-11-13

    申请号:US09725287

    申请日:2000-11-29

    IPC分类号: C12P718

    CPC分类号: C12P41/001 C12P7/18

    摘要: An industrial and economical method for obtaining a (S)-3-halogeno-1,2-propanediol, which comprises cultivating a microorganism belonging to the genus. Pseudomonas which has an ability to assimilate (R)-3-halogeno-1,2-propanediol and can grow by assimilating (R)-3-halogeno-1,2-propanediol as a single carbon source, in a culture medium containing a racemic 3-halogeno-1,2-propanediol as a substrate, and isolating the (S)-3-halogeno-1,2-propanediol from the culture medium.

    摘要翻译: 一种用于获得(S)-3-卤代-1,2-丙二醇的工业和经济方法,其包括培养属于该属的微生物。 具有同化(R)-3-卤代-1,2-丙二醇能够通过将(R)-3-卤代-1,2-丙二醇作为单一碳源同化而生长的能力的假单胞菌在含有 外消旋3-卤代-1,2-丙二醇作为底物,并从培养基中分离(S)-3-卤代-1,2-丙二醇。

    Enzymes which dehydrate glycerol
    3.
    发明授权
    Enzymes which dehydrate glycerol 有权
    脱水甘油的酶

    公开(公告)号:US06803218B1

    公开(公告)日:2004-10-12

    申请号:US09405692

    申请日:1999-09-24

    IPC分类号: C12P718

    CPC分类号: C12N9/0004 C12N9/88 C12P7/18

    摘要: The present invention relates to improved methods and reagents for the production of 1,3-propanediol. In particular, the present invention provides novel thermophilic organisms and thermostable enzymes cable of catalyzing the fermentation of glycerol to 1,3-propanediol. The present invention also relates to methods of isolating such thermophilic organisms, methods of cloning polynucleotides that encode such enzymes, polynucleotides encoding such enzymes, and methods of using such enzymes and organisms for the production of 1,3-propanediol.

    摘要翻译: 本发明涉及用于生产1,3-丙二醇的改进方法和试剂。 特别地,本发明提供催化甘油发酵成1,3-丙二醇的新型嗜热生物体和热稳定酶电缆。 本发明还涉及分离这种嗜热生物的方法,克隆编码这种酶的多核苷酸的方法,编码这种酶的多核苷酸,以及使用这些酶和生物体来生产1,3-丙二醇的方法。

    Xylitol dehydrogenase of acetic acid bacteria and gene thereof
    6.
    发明授权
    Xylitol dehydrogenase of acetic acid bacteria and gene thereof 失效
    乙酸细菌的木糖醇脱氢酶及其基因

    公开(公告)号:US06242228B1

    公开(公告)日:2001-06-05

    申请号:US09363189

    申请日:1999-07-29

    IPC分类号: C12P718

    CPC分类号: C12N9/0006 C12P7/18

    摘要: Xylitol is produced by allowing xylitol dehydrogenase or cells instoduced with a DNA coding for xylitol dehydrogenase, which is a protein of the following (A) or (B) to act on D-xylulose, and collecting produced xylitol: (A) a protein which has the amino acid sequence of SEQ ID NO: 4; (B) a protein which has the amino acid sequence of SEQ ID NO: 4 including substitution, deletion, insertion, addition, or inversion of one or several amino acids, and has xylitol dehydrogenase activity.

    摘要翻译: 通过使木糖醇脱氢酶或用编码木糖醇脱氢酶的DNA(其是以下(A)或(B)的蛋白质)作用于D-木酮糖并收集产生的木糖醇的DNA产生木糖醇:(A) 具有SEQ ID NO:4的氨基酸序列;(B)具有SEQ ID NO:4的氨基酸序列的蛋白质,包括一个或几个氨基酸的取代,缺失,插入,添加或倒位,并且具有木糖醇 脱氢酶活性。

    Process for the production of 1,3-propanediol by fermentation
    7.
    发明授权
    Process for the production of 1,3-propanediol by fermentation 有权
    通过发酵生产1,3-丙二醇的方法

    公开(公告)号:US06406895B1

    公开(公告)日:2002-06-18

    申请号:US09696622

    申请日:2000-10-25

    IPC分类号: C12P718

    CPC分类号: C12P7/18

    摘要: The present invention relates to a process for the production of 1,3-propanediol by the fermentation of a 1,3-propanediol-producing microorganism in a fermentation medium containing glucose, characterized in that the fermentation is carried out without mechanical agitation, with the maintenance of an air retention greater than or equal to 50%, expressed as the volume of gas relative to the total volume of the liquid phase of the fermentation medium, and with the maintenance of a high cell density and a microorganism viability value, determined by a test A, greater than or equal to 95%, preferably of between 95 and 99%, by controlling frothing in the fermentation medium.

    摘要翻译: 本发明涉及通过在含有葡萄糖的发酵培养基中发酵1,3-丙二醇生产微生物来生产1,3-丙二醇的方法,其特征在于发酵在没有机械搅拌的情况下进行, 维持空气保持率大于或等于50%,表示为相对于发酵培养基液相总体积的气体体积,并维持高细胞密度和微生物活力值,由 通过控制发酵培养基中的发泡,测试A大于或等于95%,优选在95至99%之间。

    Process for preparing xylitol
    8.
    发明授权
    Process for preparing xylitol 失效
    制备木糖醇的方法

    公开(公告)号:US06303353B1

    公开(公告)日:2001-10-16

    申请号:US09509547

    申请日:2000-04-12

    IPC分类号: C12P718

    CPC分类号: C12P7/18

    摘要: Xylitol is produced by contacting D-arabitol with a microorganism that belongs to the genus Gluconobacter, Acetobacter, Achromobacter, Agrobacterium, Arthrobacter, Azotobacter, Brevibacterium, Corynebacterium, Erwinia, Flavobacterium, Micrococcus, Nocardia, Planococcus, Pseudomonas, Rhodococcus, Morganella, Actinomadura, Actinomyces, or Streptomyces and is capable of converting D-arabitol to xylitol, and recovering xylitol thus produced.

    摘要翻译: 通过使D-阿拉伯糖醇与属于葡萄糖酸杆菌属,醋杆菌属,无色杆菌属,农杆菌属,节杆菌属,固氮菌属,短杆菌属,棒状杆菌属,欧文氏菌属,黄杆菌属,微球菌属,诺卡氏菌属,普兰克菌属,假单胞菌属,红球菌属,摩根氏菌属,放线菌属, 放线菌或链霉菌,能够将D-阿拉伯糖醇转化为木糖醇,并回收如此生产的木糖醇。

    Erythritol-producing yeast strains
    9.
    发明授权
    Erythritol-producing yeast strains 有权
    产生赤藓糖醇的酵母菌株

    公开(公告)号:US06300107B1

    公开(公告)日:2001-10-09

    申请号:US09585926

    申请日:2000-06-02

    IPC分类号: C12P718

    摘要: A yeast strain capable of converting glucose to erythritol, said strain having the following identifying characteristics: an absence of motile spores; septate mycelia; asexual reproduction; an absence of reniform cells; conidia optionally formed on short denticles but not on elongate stalks; an absence of ballistoconidia; non-monopolar budding on a broad base; acropetal chains of blastoconidia; dark brown, thick-walled chlamydospores; an ability to assimilate sucrose, glycerol and maltose; an inability to assimilate lactose; an inability to ferment galactose; an ability to grow in a vitamin-free medium; and an ability to grow at 25° C. to 36° C.

    摘要翻译: 能够将葡萄糖转化成赤藓糖醇的酵母菌株,所述菌株具有以下识别特征:不存在活动性孢子; 隔膜菌丝体 无性繁殖; 没有肾细胞; 分生孢子任选地形成在短的牙质上,但不在细长的茎上; 没有球囊菌 广泛基地的非单极芽; 冠状结节链; 深棕色,厚壁的衣原体; 同化蔗糖,甘油和麦芽糖的能力; 无法同化乳糖; 无法发酵半乳糖; 在无维生素的培养基中生长的能力; 和在25℃至36℃生长的能力。