摘要:
The various embodiments disclosed herein utilize multiple lasers that have different wavelengths and a single detection path. The lasers are mounted orthogonal to one another so that one laser will provide a forward angle light scatter (FALS) signal in the detection path, and one laser will provide a side scatter signal in the detection path (i.e., the single detection optics are approximately in-line with the FALS laser and approximately orthogonal to the side scatter laser). The single detector path spectrally separates the forward and side scatter signals prior to applying them to their respective detectors for measurement.
摘要:
A multichannel system for classifying particles according to one or more characteristics of the particles includes a plurality of flow cytometry units. Each flow cytometry unit is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing particles using a beam of electromagnetic radiation. Each flow cytometry unit has a sensor operable to generate a time-varying output signal indicative of at least one characteristic of the particles in the stream of fluid as the stream of fluid is interrogated by the beam of electromagnetic radiation. The flow cytometry units share an integrated platform and perhaps even a common processor for receiving and processing information from the units. The common processor is programmed to receive the time-varying output signals from the flow cytometry units substantially continuously and to process the output signals.
摘要:
A self-contained rechargeable device, such as a flashlight, utilizing a rechargeable source of electrical energy, such as rechargeable batteries, and including a pivotally mounted connector for connecting the battery and any recharging circuit connected thereto to a source of recharging energy when positioned in a recharging position and, alternatively, having a standby position internally of the device in which the connector enables a circuit between the source of energy and a load to be operated thereby. The circuit is completed by operation of a manually-operated switch connected between the energy source and the load. The load is totally disconnected from the battery during recharging. A manually-operated actuator effects movement of the connector generally between the charging position, wherein the connector completes a circuit between the rechargeable batteries and a potential source of electrical recharging energy in the standby position where the connector enables the circuit between the batteries and the load and an position wherein the actuator operates the manual switch for energizing the load.
摘要:
A system and method for sorting a mixture of stained particles in a fluid flow path, including stained particles from two distinguishable groups. The system and method can include an electromagnetic radiation source for exciting fluorescence emissions from the stained particles, a photodetector for detecting the fluorescence emissions from the stained particles, a processor for classifying the stained particles; and a photo-damaging laser for damaging selected particles in the flow path.
摘要:
A system and method for sorting a mixture of stained particles including a digital signal processor for analyzing and classifying the digital information generated from the particles and providing a sorting signal to a sorting system as a function of the analyzed and classified digital information.
摘要:
A system and method for sorting a mixture of stained particles in a fluid flow path, including stained particles from two distinguishable groups. The system and method can include an electromagnetic radiation source for exciting fluorescence emissions from the stained particles, a photodetector for detecting the fluorescence emissions from the stained particles, a processor for classifying the stained particles; and a photo-damaging laser for damaging selected particles in the flow path.
摘要:
A flow cytometry nozzle and method for orienting sperm cells in a fluid flow path including an interior surface of a nozzle. The interior surface of the nozzle having a first, a second and a third axially tapered region for progressively accelerating the speed of a fluid stream in a downstream direction toward a nozzle exit. At least two of the regions having generally elliptical cross sectional shapes oriented in different directions for applying torsional forces to a fluid stream passing there through, tending to bring sperm cells in the stream into a desired orientation.
摘要:
A flow cytometry system and method for sorting a mixture of particles with a fluid delivery system delivering a fluid containing particles, a flow cytometry apparatus for receiving the fluid, forming the fluid into a stream and using flow cytometry to classify the particles according to certain characteristics; a sorting system for sorting the particles according to the classification and according to a sorting strategy to provide at least one population containing desired particles and; a control responsive to information received from the flow cytometry apparatus for controlling the sorting system to vary its sorting strategy or for controlling the fluid delivery system to vary the rate at which fluid is delivered as a function at least one of purity and quantity of the particles.
摘要:
Machine tool systems and methods include methods of synchronizing cutting tools with a workpiece retainer. In some embodiments, the methods and systems provide at least two cutting tools that are synchronized to machine a common surface of a workpiece in a quick and efficient manner. A controller having a single line of code for controlling both cutting tools and the workpiece retainer may be used. The cutting tools may be synchronized such that they engage substantially opposite portions of the workpiece, thereby to reduce resulting forces in the workpiece that may tend to induce workpiece deflection and/or chatter. In other embodiments, a cutting tool is synchronized with a split workpiece holder that may be controlled to induce a compression, tension, or torsion pre-load in the workpiece.
摘要:
A multi-channel apparatus for classifying particles according to one or more particle characteristics may have a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units may share an integrated platform with: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method uses a plurality of flow cytometry units sharing the integrated platform to perform a flow cytometric operation, such as analyzing or sorting particles.