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    Method for generating primate trophoblasts
    1.
    发明授权
    Method for generating primate trophoblasts 有权

    公开(公告)号：US10435666B2

    公开(公告)日：2019-10-08

    申请号：US12131610

    申请日：2008-06-02

    申请人： Ren-He Xu ,  James A. Thomson

    发明人： Ren-He Xu ,  James A. Thomson

    IPC分类号： C12N5/073

    摘要： The first method to cause a culture of human and other primate stem cells to directly and uniformly differentiate into a committed cell lineage is disclosed. Treatment of primate stem cells with a single protein trophoblast induction factor causes the cells to transform into human trophoblast cells, the precursor cells of the placenta. Several protein factors including bone morphogenic protein 4 (BMP4), BMP2, BMP7, and growth and differentiation factor 5 can serve as trophoblast-inducting factors.

    Culture medium containing gamma-aminobutyric acid, pipecolic acid or lithium for the maintenance of stem cells in an undifferentiated state
    2.
    发明授权
    Culture medium containing gamma-aminobutyric acid, pipecolic acid or lithium for the maintenance of stem cells in an undifferentiated state 有权
    含有γ-氨基丁酸，哌啶酸或锂的培养基用于维持未分化状态的干细胞

    公开(公告)号：US08426203B2

    公开(公告)日：2013-04-23

    申请号：US13427548

    申请日：2012-03-22

    申请人： James A. Thomson ,  Tenneille Ludwig

    发明人： James A. Thomson ,  Tenneille Ludwig

    IPC分类号： C12N5/04

    CPC分类号： C12N5/0606 ,  C12N5/0696 ,  C12N2500/12 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/50 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/845

    摘要： Previous methods for culturing primate pluripotent stem cells have required either fibroblast feeder cells or a medium which was exposed to fibroblast feeder cells to maintain the stem cells in an undifferentiated state. It has now been found that high levels of fibroblast growth factor in a medium together with at least one of gamma aminobutyric acid, pipecolic acid, and lithium, enables pluripotent stem cells to remain undifferentiated indefinitely through multiple passages, even without feeder cells or conditioned medium. Without beta-mercaptoethanol, the medium improves cloning efficiency. Also, a matrix of human proteins can be used to culture the undifferentiated cells without exposing the cells to animal products. Further disclosed are new primate pluripotent cell lines made using the defined culture conditions, including the medium and the matrix. Such new cell lines will have never been exposed to animal cells, animal products, feeder cells or conditioned medium.

    摘要翻译： 培养灵长类多能干细胞的先前方法需要成纤维细胞饲养细胞或暴露于成纤维细胞饲养细胞的培养基以维持干细胞处于未分化状态。 现在已经发现，培养基中高水平的成纤维细胞生长因子与γ-氨基丁酸，哌啶酸和锂中的至少一种一起使多能干细胞通过多次传代无限期地保持未分化，即使没有饲养细胞或条件培养基 。 没有β-巯基乙醇，培养基提高了克隆效率。 此外，可以使用人类蛋白质的基质来培养未分化细胞而不将细胞暴露于动物产品。 进一步公开的是使用定义的培养条件制备的新的灵长类动物多能细胞系，包括培养基和基质。 这样的新细胞系从未暴露于动物细胞，动物产品，饲养细胞或条件培养基。

    MEDIUM AND CULTURE OF EMBRYONIC STEM CELLS
    4.
    发明申请
    MEDIUM AND CULTURE OF EMBRYONIC STEM CELLS 有权
    胚胎干细胞的培养和培养

    公开(公告)号：US20120178161A1

    公开(公告)日：2012-07-12

    申请号：US13427548

    申请日：2012-03-22

    申请人： James A. Thomson ,  Tenneille Ludwig

    发明人： James A. Thomson ,  Tenneille Ludwig

    IPC分类号： C12N5/071 ,  C12N5/0735

    CPC分类号： C12N5/0606 ,  C12N5/0696 ,  C12N2500/12 ,  C12N2500/20 ,  C12N2500/25 ,  C12N2500/32 ,  C12N2500/36 ,  C12N2500/38 ,  C12N2500/50 ,  C12N2500/90 ,  C12N2500/98 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/845

    摘要： Previous methods for culturing primate pluripotent stem cells have required either fibroblast feeder cells or a medium which was exposed to fibroblast feeder cells to maintain the stem cells in an undifferentiated state. It has now been found that high levels of fibroblast growth factor in a medium together with at least one of gamma aminobutyric acid, pipecolic acid, and lithium, enables pluripotent stem cells to remain undifferentiated indefinitely through multiple passages, even without feeder cells or conditioned medium. Without beta-mercaptoethanol, the medium improves cloning efficiency. Also, a matrix of human proteins can be used to culture the undifferentiated cells without exposing the cells to animal products. Further disclosed are new primate pluripotent cell lines made using the defined culture conditions, including the medium and the matrix. Such new cell lines will have never been exposed to animal cells, animal products, feeder cells or conditioned medium.

    摘要翻译： 培养灵长类多能干细胞的以前的方法需要成纤维细胞饲养细胞或暴露于成纤维细胞饲养细胞的培养基以维持干细胞处于未分化状态。 现在已经发现，培养基中高水平的成纤维细胞生长因子与γ-氨基丁酸，哌啶酸和锂中的至少一种一起使多能干细胞通过多次传代无限期地保持未分化，即使没有饲养细胞或条件培养基 。 没有β-巯基乙醇，培养基提高了克隆效率。 此外，可以使用人类蛋白质的基质来培养未分化细胞而不将细胞暴露于动物产品。 进一步公开的是使用定义的培养条件制备的新的灵长类动物多能细胞系，包括培养基和基质。 这样的新细胞系从未暴露于动物细胞，动物产品，饲养细胞或条件培养基。

    Method of forming myeloid precursor cells from human embryonic stem cells
    5.
    发明授权
    Method of forming myeloid precursor cells from human embryonic stem cells 有权
    从人胚胎干细胞形成骨髓前体细胞的方法

    公开(公告)号：US08133732B2

    公开(公告)日：2012-03-13

    申请号：US12876830

    申请日：2010-09-07

    申请人： Igor I. Slukvin ,  James A. Thomson ,  Maksym A. Vodyanyk ,  Maryna E. Gumenyuk

    发明人： Igor I. Slukvin ,  James A. Thomson ,  Maksym A. Vodyanyk ,  Maryna E. Gumenyuk

    IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/071

    CPC分类号： C12N5/0639 ,  C12N5/064 ,  C12N2506/02

    摘要： This invention relates to the culture of dendritic cells from human embryonic stem (ES) cells. Human ES cells are first cultured into hematopoietic cells by co-culture with stromal cells. The cells now differentiated into the hematopoietic lineage are then cultured with GM-CSF to create a culture of myeloid precursor cells. Culture of the myeloid precursor cells with the cytokines GM-CSF and IL-4 causes functional dendritic cells to be generated. The dendritic cells have a unique phenotype, as indicated by their combination of cell surface markers.

    摘要翻译： 本发明涉及来自人胚胎干（ES）细胞的树突状细胞的培养。 首先通过与基质细胞共培养将人类ES细胞培养成造血细胞。 然后将分化成造血谱系的细胞与GM-CSF一起培养以产生骨髓前体细胞的培养物。 用细胞因子GM-CSF和IL-4培养骨髓前体细胞会导致产生功能性树突状细胞。 树突细胞具有独特的表型，如细胞表面标志物的组合所示。

    Primate embryonic stem cell line
    9.
    发明授权
    Primate embryonic stem cell line 失效
    灵长类胚胎干细胞系

    公开(公告)号：US07582479B2

    公开(公告)日：2009-09-01

    申请号：US11036245

    申请日：2005-01-14

    申请人： James A. Thomson

    发明人： James A. Thomson

    IPC分类号： C12N15/02 ,  C12N15/04

    CPC分类号： C12N5/0605 ,  C12N5/0603 ,  C12N5/0606 ,  C12N2502/13 ,  C12N2506/02

    摘要： A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (−); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a particularly advantageous embodiment, the cells of the preparation are human embryonic stem cells, have normal karyotypes, and continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). A method for isolating a primate embryonic stem cell line is also disclosed.

    摘要翻译： 公开了灵长类动物胚胎干细胞的纯化制剂。 该制剂的特征在于以下细胞表面标志物：SSEA-1（ - ）; SSEA-4（+）; TRA-1-60（+）; TRA-1-81（+）; 和碱性磷酸酶（+）。 在一个特别有利的实施方案中，制剂的细胞是人胚胎干细胞，具有正常的核型，并且在连续培养11个月后，以未分化状态继续增殖。 胚胎干细胞系还保留了整个培养物中形成滋养细胞并分化成来自所有三个胚胎胚层（内胚层，中胚层和外胚层）的所有组织的能力。 还公开了分离灵长类动物胚胎干细胞系的方法。

    Feeder independent extended culture of embryonic stem cells
    10.
    发明授权
    Feeder independent extended culture of embryonic stem cells 有权
    饲养者独立扩展胚胎干细胞培养

    公开(公告)号：US07514260B2

    公开(公告)日：2009-04-07

    申请号：US11134564

    申请日：2005-05-20

    申请人： Ren-He Xu ,  James A. Thomson

    发明人： Ren-He Xu ,  James A. Thomson

    IPC分类号： C12N5/00 ,  C12N5/02 ,  C12N5/08

    CPC分类号： C12N5/0606 ,  C12N2501/115 ,  C12N2501/155

    摘要： Previous methods for culturing human embryonic stem cells have required either fibroblast feeder cells or a medium which has been exposed to fibroblast feeder cells in order to maintain the stem cells in an undifferentiated state. It has now been found that if an antagonist of bone morphogenic protein is added to the medium in which the stem cells are cultured, together with fibroblast growth factor, the stem cells will remain undifferentiated indefinitely, even without feeder cells or conditioned medium.

    摘要翻译： 用于培养人胚胎干细胞的以前的方法需要成纤维细胞饲养细胞或已经暴露于成纤维细胞饲养细胞的培养基，以便保持干细胞处于未分化状态。 现在已经发现，如果将骨形态发生蛋白的拮抗剂加入到其中培养干细胞的培养基中，与成纤维细胞生长因子一起，干细胞将无限期地保持未分化，即使没有饲养细胞或条件培养基。