公开(公告)号：US07485212B2

公开(公告)日：2009-02-03

申请号：US10487694

申请日：2002-08-12

申请人： Itamar Willner ,  Evgeny Katz

发明人： Itamar Willner ,  Evgeny Katz

IPC分类号： G01N27/327

CPC分类号： H01M8/16 ,  C12Q1/004 ,  Y02E60/527

摘要： The present invention provides a system for the determination of an analyte in a liquid medium. The system comprises a self-powered biosensor and a detector for measuring an electrical signal generated by said biosensor while the analyte is being oxidized or reduced, the biosensor comprising a pair of electrodes, one of the electrodes being an anode and the other a cathode, both of which carry redox enzymes on their surface. An enzyme carried on one of the electrodes can catalyze an oxidation or reduction reaction in which the analyte is oxidized or reduced, respectively, and the other of said pair of electrodes carries on its surface enzymes that can catalyze a reaction in which the oxidizer or the reducer are reduced or oxidized, respectively, in the presence of the analyte.

摘要翻译： 本发明提供了一种用于确定液体介质中的分析物的系统。 该系统包括自供电的生物传感器和用于在分析物被氧化或还原时测量由所述生物传感器产生的电信号的检测器，所述生物传感器包括一对电极，一个电极是阳极，另一个是阴极， 两者都在其表面携带氧化还原酶。 携带在其中一个电极上的酶可以催化氧化或还原反应，其中分析物分别被氧化或还原，并且所述一对电极中的另一个在其表面上承载可以催化反应的酶，其中氧化剂或 还原剂分别在分析物存在下还原或氧化。

公开(公告)号：US20050260350A1

公开(公告)日：2005-11-24

申请号：US10466070

申请日：2002-01-07

申请人： Andrew Shipway ,  Itamar Willner

发明人： Andrew Shipway ,  Itamar Willner

IPC分类号： C23C18/18 ,  C23C18/16 ,  C23C18/31 ,  H05K3/10 ,  H05K3/18 ,  H05K3/46 ,  B05D5/12 ,  B05D3/10

CPC分类号： C23C18/1608 ,  C23C18/1644 ,  C23C18/30 ,  H05K3/102

摘要： Disclosed is a process for forming a conductor pattern on a face of a substrate, which is typically sorbing and porous, such as paper or cloth. The method comprises forming on the surface an exposed pattern of colloid particles, corresponding to the conductor pattern to be formed. The colloid particles suitable for use according to the invention are those that are capable of catalyzing electroless deposition of copper, silver, gold, and the like. After the exposed pattern of colloid particles is formed, precipitation of a metal substance on said face is caused, to form said conductor pattern.

摘要翻译： 公开了一种在基材的表面上形成导体图案的方法，其通常是吸附和多孔的，例如纸或布。 该方法包括在表面上形成对应于要形成的导体图案的暴露的胶体颗粒图案。 适合根据本发明使用的胶体颗粒是能够催化铜，银，金等的无电沉积的胶体颗粒。 在形成胶体颗粒的暴露图案之后，引起金属物质在所述面上的沉淀，形成所述导体图案。

公开(公告)号：US06630309B2

公开(公告)日：2003-10-07

申请号：US08983570

申请日：1998-06-04

申请人： Itamar Willner ,  Shlomo Levi ,  Yael Cohen ,  Eugenii Katz ,  Arie Dagan

发明人： Itamar Willner ,  Shlomo Levi ,  Yael Cohen ,  Eugenii Katz ,  Arie Dagan

IPC分类号： G01N33553

CPC分类号： G01N33/54373

摘要： Binding between two members of a recognition pair, e.g. antigen-antibody is determined by utilizing a probe which includes a piezoelectric crystal with electrodes on two opposite faces of the crystal. The crystal carries one or more metal plates which may be the same or different than the electrodes, the metal plates having immobilized thereon a first member of a recognition pair. Binding of a second member of the recognition pair to the first member, or dissociation between the two members and release of the second member from the probe, causes a change of immobilized mass which results in a change to the probe's resonance frequency. The immobilized members may be immobilized on the surface of the metal plates by means of a linking group, having the following general formula (I): Z—R1—Q, wherein Z represents a sulphur-containing moiety which is capable of chemical association with, attachment to or chemisorption onto the metal, R1 represents a connecting group, Q is a functional group which is capable of forming a covalent bond with a moiety of said first member of the recognition pair.

摘要翻译： 识别对的两个成员之间的绑定，例如 通过利用包含晶体的两个相对面上的电极的压电晶体的探针来测定抗原抗体。 晶体携带一个或多个可以与电极相同或不同的金属板，金属板固定有识别对的第一构件。 识别对的第二个成员与第一个成员的结合，或两个成员之间的解离和第二个成员从探针的释放导致固定的质量的变化，导致探针的共振频率的变化。 可以通过具有以下通式（I）的连接基团将固定化的构件固定在金属板的表面上：ZR 1 -Q，其中Z表示能够化学结合的含硫部分 与金属的附着或化学吸附，R 1表示连接基团，Q是能够与识别对的所述第一成员的部分形成共价键的官能团。

公开(公告)号：US06214205B1

公开(公告)日：2001-04-10

申请号：US09117256

申请日：1998-12-15

申请人： Itamar Willner ,  Eugenii Katz ,  Yael Cohen ,  Ron Blonder

发明人： Itamar Willner ,  Eugenii Katz ,  Yael Cohen ,  Ron Blonder

IPC分类号： G01N2726

CPC分类号： C12Q1/003 ,  G01N33/5438

摘要： Electrochemical determination of the presence, and optionally concentration of an analyte utilizes an electrode having immobilized thereon one member of a recognition pair, the recognition pair consisting of said analyte. In the presence of the analyte medium pair complexes, which are complexes between the analyte and the immobilized member are formed. The electrode forms part of an electrochemical system which includes also redox molecules comprising at least one first redox molecule and at least one second redox molecule, the former transferring electrons between the electrode and the latter. Upon formation of a pair complex the electron transfer is inhibited and the change in the electrical response then indicates the presence of said analyte in the medium.

摘要翻译： 分析物的存在和任选地浓度的电化学测定使用其上固定有识别对的一个成员的电极，识别对由所述分析物组成。 在存在分析物的情况下，形成了分析物与固定化部件之间的复合物。 电极形成电化学系统的一部分，其还包括包含至少一个第一氧化还原分子和至少一个第二氧化还原分子的氧化还原分子，前者在电极和后者之间转移电子。 在形成一对复合物时，电子转移被抑制，并且电响应的变化然后指示所述分析物在介质中的存在。

公开(公告)号：US07135295B1

公开(公告)日：2006-11-14

申请号：US09889936

申请日：2000-01-25

申请人： Itamar Willner ,  Zelig Eshhar

发明人： Itamar Willner ,  Zelig Eshhar

IPC分类号： G01N33/53

CPC分类号： C07K16/00 ,  G01N33/54373

摘要： A protein comprising an antigen-binding portion formed by two cooperating peptide sequences of FIGS. 3A and 3B of the application. The protein may alternatively comprise an altered antigen-binding portion where at least one of the peptide sequences is an altered sequence, an altered sequence being a sequence of FIG. 3A or 3B in which one or more of an amino acid residue has been added, deleted or replaced by another amino acid residue. The altered antigen-binding portion retains substantially the same antigen-binding specificity as said antigen-binding portion. Also disclosed are apparatus, systems and methods for detecting small assayed molecules in a sample using the protein.

摘要翻译： 包含由图1和2的两个协同肽序列形成的抗原结合部分的蛋白质。 3 A和3 B的应用程序。 蛋白质可以可选地包含改变的抗原结合部分，其中至少一个肽序列是改变的序列，改变的序列是图1的序列。 3 A或3 B，其中一个或多个氨基酸残基已经被另一个氨基酸残基加入，缺失或替换。 改变的抗原结合部分保持与所述抗原结合部分基本相同的抗原结合特异性。 还公开了用于使用蛋白质检测样品中的小测定分子的装置，系统和方法。

公开(公告)号：US20050130248A1

公开(公告)日：2005-06-16

申请号：US10503465

申请日：2003-01-30

申请人： Itamar Willner ,  Eugenii Katz ,  Maya Zayats

发明人： Itamar Willner ,  Eugenii Katz ,  Maya Zayats

IPC分类号： C12Q1/00 ,  C12Q1/54

CPC分类号： C12Q1/004

摘要： The present invention concerns an electrode carrying immobilized redox enzymes such that electric charge can flow between an electron mediator group to the enzyme cofactor by the use of boronic acid or a boronic acid derivative that acts as a linker moiety between the cofactor and the electron mediator group. The invention also concerns devices and systems that make use of the electrode of the invention, such as biosensors and fuel cells, the electrode being one of the components thereof.

摘要翻译： 本发明涉及携带固定化氧化还原酶的电极，使得电荷可以通过使用硼酸或作为辅因子和电子介体基团之间的连接体部分的硼酸衍生物在电子介体基团与酶辅因子之间流动 。 本发明还涉及利用本发明的电极，例如生物传感器和燃料电池的电极是其组成部分之一的装置和系统。

公开(公告)号：US5443701A

公开(公告)日：1995-08-22

申请号：US109922

申请日：1993-08-23

申请人： Itamar Willner ,  Evgeny Katz ,  Azalia Riklin ,  Ron Kasher ,  Benjamin Shoham

发明人： Itamar Willner ,  Evgeny Katz ,  Azalia Riklin ,  Ron Kasher ,  Benjamin Shoham

IPC分类号： G01N27/327 ,  C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/004 ,  C12Q1/003 ,  Y10S435/817

摘要： An analytical method for determining the presence or concentration of an analyte in a liquid medium is provided in which electrons are transferred from an electrode material of an electrode to the redox center of an enzyme, by the mediation of an electron mediator, whereby in the presence of an analyte the enzyme calalyzed a redox reaction in which the analyte is converted into a product. By measuring either the concentration or the product or the charge which flows in this process, the presence and/or concentration of the analyte in the medium is determined. Either or both of the enzyme and the electron mediator are immobilized on the electrode.

摘要翻译： 提供了一种用于确定液体介质中分析物的存在或浓度的分析方法，其中电子通过电子介体的介导从电极的电极材料转移到酶的氧化还原中心，由此在存在 的分析物，酶催化氧化还原反应，其中分析物转化成产物。 通过测量在该过程中流动的浓度或产物或电荷，确定介质中分析物的存在和/或浓度。 酶和电子介体中的任一个或两者被固定在电极上。

公开(公告)号：US20110177606A1

公开(公告)日：2011-07-21

申请号：US13001972

申请日：2009-06-30

申请人： Itamar Willner ,  Ran Tel-Vered ,  Michael Riskin

发明人： Itamar Willner ,  Ran Tel-Vered ,  Michael Riskin

IPC分类号： G01N33/22 ,  G01N27/00 ,  B82Y30/00

CPC分类号： G01N33/0057 ,  G01N2600/00 ,  Y10T436/173076

摘要： An ultrasensitive method for detecting analytes in a sample is provided. The method involves the use of a matrix of nanoparticles which are associated with recognition groups capable of undergoing interaction with the analyte.

摘要翻译： 提供了一种用于检测样品中分析物的超灵敏方法。 该方法涉及使用与能够与分析物相互作用的识别基团相关联的纳米颗粒的基质。

公开(公告)号：US20070254282A1

公开(公告)日：2007-11-01

申请号：US10588153

申请日：2005-01-27

申请人： Itamar Willner ,  Yi Xiao ,  Valeri Pavlov ,  Tamara Niazov

发明人： Itamar Willner ,  Yi Xiao ,  Valeri Pavlov ,  Tamara Niazov

IPC分类号： C12Q1/68

CPC分类号： C12N15/115 ,  C12Q1/6813 ,  C12Q1/683 ,  C12Q2521/543 ,  C12Q2521/331

摘要： A method for the determination of an analyte in a sample, the method comprising: (a) providing a catalytic polynucleotide; (b) contacting the catalytic polynucleotide with the sample so that the catalytic polynucleotide may bind to the analyte; (c) providing assay conditions such that the catalytic polynucleotide produces an optically detectable signal in the presence of the analyte; and (d) detecting the signal, thereby determining the presence of the analyte in the sample. One application of the method is for the detection of telomerase in a biological sample.

摘要翻译： 一种用于测定样品中分析物的方法，所述方法包括：（a）提供催化多核苷酸; （b）使催化多核苷酸与样品接触，使得催化多核苷酸可以结合分析物; （c）提供测定条件，使得催化多核苷酸在分析物存在下产生可光学检测的信号; 和（d）检测信号，从而确定样品中分析物的存在。 该方法的一个应用是用于检测生物样品中的端粒酶。

公开(公告)号：US07018518B2

公开(公告)日：2006-03-28

申请号：US10133635

申请日：2002-04-29

申请人： Itamar Willner ,  Eugenii Katz ,  Maya Zayats

发明人： Itamar Willner ,  Eugenii Katz ,  Maya Zayats

IPC分类号： G01N27/327 ,  H01M8/16

CPC分类号： H01M8/16 ,  C12Q1/003 ,  H01M4/9008 ,  Y02E60/527

摘要： The present invention concerns an electrode carrying immobilized redox enzymes such that electric charge can flow between an electron mediator group to the enzyme cofactor by the use of boronic acid or a boronic acid derivative that acts as a linker moiety between the cofactor and the electron mediator group. The invention also concerns devices and systems that make use of the electrode of the invention, such as bio-sensors and fuel cells, the electrode being one of the components thereof.