公开(公告)号：US07018518B2

公开(公告)日：2006-03-28

申请号：US10133635

申请日：2002-04-29

申请人： Itamar Willner ,  Eugenii Katz ,  Maya Zayats

发明人： Itamar Willner ,  Eugenii Katz ,  Maya Zayats

IPC分类号： G01N27/327 ,  H01M8/16

CPC分类号： H01M8/16 ,  C12Q1/003 ,  H01M4/9008 ,  Y02E60/527

摘要： The present invention concerns an electrode carrying immobilized redox enzymes such that electric charge can flow between an electron mediator group to the enzyme cofactor by the use of boronic acid or a boronic acid derivative that acts as a linker moiety between the cofactor and the electron mediator group. The invention also concerns devices and systems that make use of the electrode of the invention, such as bio-sensors and fuel cells, the electrode being one of the components thereof.

公开(公告)号：US20050130248A1

公开(公告)日：2005-06-16

申请号：US10503465

申请日：2003-01-30

申请人： Itamar Willner ,  Eugenii Katz ,  Maya Zayats

发明人： Itamar Willner ,  Eugenii Katz ,  Maya Zayats

IPC分类号： C12Q1/00 ,  C12Q1/54

CPC分类号： C12Q1/004

摘要： The present invention concerns an electrode carrying immobilized redox enzymes such that electric charge can flow between an electron mediator group to the enzyme cofactor by the use of boronic acid or a boronic acid derivative that acts as a linker moiety between the cofactor and the electron mediator group. The invention also concerns devices and systems that make use of the electrode of the invention, such as biosensors and fuel cells, the electrode being one of the components thereof.

摘要翻译： 本发明涉及携带固定化氧化还原酶的电极，使得电荷可以通过使用硼酸或作为辅因子和电子介体基团之间的连接体部分的硼酸衍生物在电子介体基团与酶辅因子之间流动 。 本发明还涉及利用本发明的电极，例如生物传感器和燃料电池的电极是其组成部分之一的装置和系统。

公开(公告)号：US20060269826A1

公开(公告)日：2006-11-30

申请号：US10547617

申请日：2004-03-02

申请人： Eugenii Katz ,  Itamar Willner

发明人： Eugenii Katz ,  Itamar Willner

IPC分类号： H01M4/90 ,  G01N33/487

CPC分类号： H01M4/9008 ,  C12Q1/001 ,  H01M4/8647 ,  H01M4/90 ,  H01M8/16 ,  Y02E60/527

摘要： The present invention provides a novel electrode carrying on at least a portion of its support surface a hybrid polymer matrix (HPM), a catalyst that can catalyze a redox reaction and an optional electron mediator group that enhances the electrical contact between the HPM and the catalyst, the HPM being capable to be electrochemically changed from a non-conductive state to a conductive state. The electrode of the invention may be used in electrical devices such as fuel cells, thus imparting them switchable and tunable properties. The fuel cell of the invention may be used as a power source or as a self-powered sensor.

摘要翻译： 本发明提供了一种新型电极，其载体的至少一部分载体是杂化聚合物基质（HPM），可以催化氧化还原反应的催化剂和任选的电子介体基团，其增强了HPM和催化剂之间的电接触 ，HPM能够电化学地从非导电状态改变到导电状态。 本发明的电极可用于诸如燃料电池的电气装置中，从而赋予它们可切换和可调谐的特性。 本发明的燃料电池可以用作电源或作为自供电传感器。

公开(公告)号：US20060199240A1

公开(公告)日：2006-09-07

申请号：US10536032

申请日：2003-10-02

申请人： Itarmar Willner ,  Eugenii Katz ,  Vered Yissar

发明人： Itarmar Willner ,  Eugenii Katz ,  Vered Yissar

IPC分类号： C12Q1/54 ,  C12Q1/26 ,  C12M1/34

CPC分类号： C12Q1/001

摘要： The present invention provides a method and a device that utilizes functionalized semiconductor element for detecting presence and/or concentration of an agent in an assayed sample. The device of the present invention comprises: (i) a body having a surface comprising or having associated thereto semi-conducting material that can be excited such that in the presence of an electron donor, said semi-conducting material can generate an electric current within the body; and (ii) an enzyme attached to said semi-conducting material which in the presence of a substrate said enzyme catalyzes a reaction that yields said electron donors.

摘要翻译： 本发明提供一种利用功能化的半导体元件检测测定样品中的试剂的存在和/或浓度的方法和装置。 本发明的装置包括：（i）具有包括半导电材料或与之有关的半导电材料的表面的主体，其可以被激发，使得在存在电子给体的情况下，所述半导体材料可以在其内产生电流 身体; 和（ii）附着到所述半导体材料上的酶，其在底物存在下催化产生所述电子给体的反应。

公开(公告)号：US06630309B2

公开(公告)日：2003-10-07

申请号：US08983570

申请日：1998-06-04

申请人： Itamar Willner ,  Shlomo Levi ,  Yael Cohen ,  Eugenii Katz ,  Arie Dagan

发明人： Itamar Willner ,  Shlomo Levi ,  Yael Cohen ,  Eugenii Katz ,  Arie Dagan

IPC分类号： G01N33553

CPC分类号： G01N33/54373

摘要： Binding between two members of a recognition pair, e.g. antigen-antibody is determined by utilizing a probe which includes a piezoelectric crystal with electrodes on two opposite faces of the crystal. The crystal carries one or more metal plates which may be the same or different than the electrodes, the metal plates having immobilized thereon a first member of a recognition pair. Binding of a second member of the recognition pair to the first member, or dissociation between the two members and release of the second member from the probe, causes a change of immobilized mass which results in a change to the probe's resonance frequency. The immobilized members may be immobilized on the surface of the metal plates by means of a linking group, having the following general formula (I): Z—R1—Q, wherein Z represents a sulphur-containing moiety which is capable of chemical association with, attachment to or chemisorption onto the metal, R1 represents a connecting group, Q is a functional group which is capable of forming a covalent bond with a moiety of said first member of the recognition pair.

摘要翻译： 识别对的两个成员之间的绑定，例如 通过利用包含晶体的两个相对面上的电极的压电晶体的探针来测定抗原抗体。 晶体携带一个或多个可以与电极相同或不同的金属板，金属板固定有识别对的第一构件。 识别对的第二个成员与第一个成员的结合，或两个成员之间的解离和第二个成员从探针的释放导致固定的质量的变化，导致探针的共振频率的变化。 可以通过具有以下通式（I）的连接基团将固定化的构件固定在金属板的表面上：ZR 1 -Q，其中Z表示能够化学结合的含硫部分 与金属的附着或化学吸附，R 1表示连接基团，Q是能够与识别对的所述第一成员的部分形成共价键的官能团。

公开(公告)号：US06214205B1

公开(公告)日：2001-04-10

申请号：US09117256

申请日：1998-12-15

申请人： Itamar Willner ,  Eugenii Katz ,  Yael Cohen ,  Ron Blonder

发明人： Itamar Willner ,  Eugenii Katz ,  Yael Cohen ,  Ron Blonder

IPC分类号： G01N2726

CPC分类号： C12Q1/003 ,  G01N33/5438

摘要： Electrochemical determination of the presence, and optionally concentration of an analyte utilizes an electrode having immobilized thereon one member of a recognition pair, the recognition pair consisting of said analyte. In the presence of the analyte medium pair complexes, which are complexes between the analyte and the immobilized member are formed. The electrode forms part of an electrochemical system which includes also redox molecules comprising at least one first redox molecule and at least one second redox molecule, the former transferring electrons between the electrode and the latter. Upon formation of a pair complex the electron transfer is inhibited and the change in the electrical response then indicates the presence of said analyte in the medium.

摘要翻译： 分析物的存在和任选地浓度的电化学测定使用其上固定有识别对的一个成员的电极，识别对由所述分析物组成。 在存在分析物的情况下，形成了分析物与固定化部件之间的复合物。 电极形成电化学系统的一部分，其还包括包含至少一个第一氧化还原分子和至少一个第二氧化还原分子的氧化还原分子，前者在电极和后者之间转移电子。 在形成一对复合物时，电子转移被抑制，并且电响应的变化然后指示所述分析物在介质中的存在。