Rapid identification of microbial agents
    2.
    发明申请
    Rapid identification of microbial agents 审中-公开
    快速鉴定微生物剂

    公开(公告)号:US20060259249A1

    公开(公告)日:2006-11-16

    申请号:US11073362

    申请日:2005-03-03

    IPC分类号: G06F19/00

    CPC分类号: G16B40/00 G16B25/00 G16B30/00

    摘要: The method described herein relate generally the identification of bioagents on the basis of base composition signatures for bioagent-identifying amplicons. Specifically, methods of the present invention are directed to the application of pattern recognition models, particularly probability pattern classifiers, to the identification of both known and previously unrecognized bioagents. In certain embodiments, the pattern classifiers relate to probability cloud patterns, such as mutational probability patterns. In other embodiments the pattern classifiers relate to polytope pattern models.

    摘要翻译: 本文所述的方法一般涉及基于用于生物鉴别扩增子的碱基组成特征的生物标记的鉴定。 具体地,本发明的方法涉及模式识别模型,特别是概率模式分类器在识别已知和以前未被认识的生物标签的应用。 在某些实施例中,模式分类器涉及概率云模式,例如突变概率模式。 在其他实施例中,模式分类器涉及多面体图案模型。

    Methods of rapid detection and identification of bioagents using microRNA
    9.
    发明申请
    Methods of rapid detection and identification of bioagents using microRNA 审中-公开
    使用microRNA快速检测和鉴定生物标本的方法

    公开(公告)号:US20050123952A1

    公开(公告)日:2005-06-09

    申请号:US10933928

    申请日:2004-09-03

    摘要: Methods for detecting and identifying unknown bioagents, including bacteria, viruses and the like, by a combination of microRNA containing nucleic acid amplification and molecular weight determination using primers which hybridize to conserved sequence regions of microRNA containing nucleic acids derived from a bioagent and which bracket variable sequence regions that uniquely identify the bioagent. The result is a “base composition signature” (BCS) or molecular mass which is then matched against a database of base composition signatures or molecular masses, by which the species of the bioagent is identified.

    摘要翻译: 通过使用与含有衍生自生物制剂的含有核酸的微RNA的保守序列区杂交的引物与包含核酸扩增的微小RNA的组合来检测和识别未知生物标签(包括细菌,病毒等)的方法, 唯一识别生物标签的序列区域。 结果是“基础组成特征”(BCS)或分子量,然后与基础组成特征或分子量的数据库进行匹配,通过该数据库识别出生物代谢物种。