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    Diffusion gradient bioreactor and extracorporeal liver device using a three-dimensional liver tissue
    2.
    发明授权
    Diffusion gradient bioreactor and extracorporeal liver device using a three-dimensional liver tissue 失效
    扩散梯度生物反应器和体外肝脏装置使用三维肝组织

    公开(公告)号：US5827729A

    公开(公告)日：1998-10-27

    申请号：US636591

    申请日：1996-04-23

    Applicant: Brian A. Naughton ,  Craig R. Halberstadt ,  Benson Sibanda

    Inventor： Brian A. Naughton ,  Craig R. Halberstadt ,  Benson Sibanda

    IPC: C12M1/00 ,  A01N1/02 ,  A61F2/02 ,  A61M1/34 ,  A61M1/36 ,  C12M1/16 ,  C12M3/06 ,  C12N5/00 ,  C12N5/071 ,  B01D63/00

    CPC classification number: C12M29/04 ,  A61F2/022 ,  A61M1/3489 ,  C12M21/08 ,  C12M25/14 ,  C12M35/04 ,  C12M35/08 ,  C12M41/32 ,  C12N5/0671 ,  C12N2500/02 ,  C12N2500/32

    Abstract: A tissue engineering bioreactor is disclosed for growing three-dimensional tissue. Cells are seeded onto a mesh and provided with two media flows, each contacting a different side of the cells. The media flows contain different concentrations of nutrients, allowing nutrients to be delivered to the cells by diffusion gradient. The bioreactor can be used to grow liver tissue, and designed as an extracorporeal liver assist device in which blood or plasma is exposed to the three-dimensional liver tissue. The blood or plasma from a patient directed to flow against the liver tissue. The liver tissue is further exposed on its opposite side to media providing nutrients and gases. The device provides porous boundaries between the blood or plasma, tissue, and media, allowing nutrient and protein delivery by diffusion gradient to dialyze a patient's blood.

    Abstract translation: 公开了用于生长三维组织的组织工程生物反应器。 将细胞接种到网状物上，并提供两个培养基流，每个接触细胞的不同侧。 培养基流中含有不同浓度的营养物质，允许通过扩散梯度将营养物质递送到细胞。 生物反应器可用于生长肝组织，并被设计为体外肝辅助装置，其中血液或血浆暴露于三维肝组织。 来自患者的血液或血浆导向流向肝组织。 肝脏组织进一步暴露在与提供营养和气体的介质的相对侧。 该装置在血液或血浆，组织和培养基之间提供多孔边界，允许营养和蛋白质通过扩散梯度递送以透析患者的血液。

    Bioreactor system
    3.
    发明授权
    Bioreactor system 失效
    生物反应器系统

    公开(公告)号：US5081035A

    公开(公告)日：1992-01-14

    申请号：US654418

    申请日：1991-02-11

    Applicant: Craig R. Halberstadt ,  A. Rees Midgley

    Inventor： Craig R. Halberstadt ,  A. Rees Midgley

    IPC: C12M3/06

    CPC classification number: C12M29/10 ,  C12M23/24 ,  C12M29/12 ,  C12M35/04 ,  C12M35/08 ,  C12M41/00 ,  C12M41/32

    Abstract: A system for continuous perfusion culturing of anchorage-dependent and anchorage-independent mammalian cell lines, inter alia, wherein in vivo capillary bed conditions are simulated by providing a bioreactor having a growth chamber wherein the cells are grown between a multitubular array comprising at least three functionally separate, inert non-degradable and structurally strong tubes or sets of tubes. A constant nutrient gradient is maintained along the entire length of the tubes by perfusing medium through the tubes at a flow rate which is sufficient to expose all areas of the chamber to fresh medium by convective forces rather than diffusion. Oxygen transfer and removal of toxic wastes is improved by the invention herein. In a preferred embodiment, porous inert expanded Teflon tubes are employed having an inner diameter of approximately between 1 and 2 millimeters.

    Abstract translation: 一种用于连续灌注培养锚定依赖和不依赖锚定的哺乳动物细胞系的系统，其中特别地，其中通过提供具有生长室的生物反应器来模拟体内毛细管床条件，其中所述细胞在包含至少三个 功能分离，惰性不可降解和结构强的管或套管。 通过使培养基通过管子以足够的流量使培养基通过对流力而不是扩散将培养室的所有区域暴露于新鲜培养基的流速，保持恒定的养分梯度。 本发明改善了有毒废物的氧气转移和去除。 在一个优选的实施方式中，使用内径大约在1和2毫米之间的多孔惰性膨胀的特氟隆管。

    Method for culturing three-dimensional tissue in diffusion gradient bioreactor and use thereof
    6.
    发明授权
    Method for culturing three-dimensional tissue in diffusion gradient bioreactor and use thereof 失效
    在扩散梯度生物反应器中培养三维组织的方法及其用途

    公开(公告)号：US06218182B1

    公开(公告)日：2001-04-17

    申请号：US09470317

    申请日：1999-12-21

    Applicant: Brian A. Naughton ,  Craig R. Halberstadt ,  Benson Sibanda

    Inventor： Brian A. Naughton ,  Craig R. Halberstadt ,  Benson Sibanda

    IPC: A01N102

    CPC classification number: C12M29/04 ,  A61F2/022 ,  A61M1/3489 ,  C12M21/08 ,  C12M25/14 ,  C12M35/04 ,  C12M35/08 ,  C12M41/32 ,  C12N5/0671 ,  C12N2500/02 ,  C12N2500/32

    Abstract: A tissue engineering bioreactor is disclosed for growing three-dimensional tissue. Cells are seeded onto a mesh and provided with two media flows, each contacting a different side of the cells. The media flows contain different concentrations of nutrients, allowing nutrients to be delivered to the cells by diffusion gradient. The bioreactor can be used to grow liver tissue, and designed as an extracorporeal liver assist device in which blood or plasma is exposed to the three-dimensional liver tissue. The blood or plasma from a patient directed to flow against the liver tissue. The liver tissue is further exposed on its opposite side to media providing nutrients and gases. The device provides porous boundaries between the blood or plasma, tissue, and media; allowing nutrient and protein delivery by diffusion gradient to dialyze a patient's blood.

    Abstract translation: 公开了用于生长三维组织的组织工程生物反应器。 将细胞接种到网状物上，并提供两个培养基流，每个接触细胞的不同侧。 培养基流中含有不同浓度的营养物质，允许通过扩散梯度将营养物质递送到细胞。 生物反应器可用于生长肝组织，并被设计为体外肝辅助装置，其中血液或血浆暴露于三维肝组织。 来自患者的血液或血浆导向流向肝组织。 肝脏组织进一步暴露在与提供营养和气体的介质的相对侧。 该装置在血液或血浆，组织和培养基之间提供多孔边界; 允许营养和蛋白质通过扩散梯度递送以透析患者的血液。

    Diffusion gradient bioreactor and extracorporeal liver device
    7.
    发明授权
    Diffusion gradient bioreactor and extracorporeal liver device 失效
    扩散梯度生物反应器和体外肝脏装置

    公开(公告)号：US6008049A

    公开(公告)日：1999-12-28

    申请号：US136503

    申请日：1998-08-19

    Applicant: Brian A. Naughton ,  Craig R. Halberstadt ,  Benson Sibanda

    Inventor： Brian A. Naughton ,  Craig R. Halberstadt ,  Benson Sibanda

    IPC: C12M1/00 ,  A01N1/02 ,  A61F2/02 ,  A61M1/34 ,  A61M1/36 ,  C12M1/16 ,  C12M3/06 ,  C12N5/00 ,  C12N5/071 ,  C12M3/00

    CPC classification number: C12M29/04 ,  A61F2/022 ,  A61M1/3489 ,  C12M21/08 ,  C12M25/14 ,  C12M35/04 ,  C12M35/08 ,  C12M41/32 ,  C12N5/0671 ,  C12N2500/02 ,  C12N2500/32

    Abstract: A tissue engineering bioreactor is disclosed for growing three-dimensional tissue. Cells are seeded onto a mesh and provided with two media flows, each contacting a different side of the cells. The media flows contain different concentrations of nutrients, allowing nutrients to be delivered to the cells by diffusion gradient. The bioreactor can be used to grow liver tissue, and designed as an extracorporeal liver assist device in which blood or plasma is exposed to the three-dimensional liver tissue. The blood or plasma from a patient directed to flow against the liver tissue. The liver tissue is further exposed on its opposite side to media providing nutrients and gases. The device provides porous boundaries between the blood or plasma, tissue, and media, allowing nutrient and protein delivery by diffusion gradient to dialyze a patient's blood.

    Abstract translation: 公开了用于生长三维组织的组织工程生物反应器。 将细胞接种到网状物上，并提供两个培养基流，每个接触细胞的不同侧。 培养基流中含有不同浓度的营养物质，允许通过扩散梯度将营养物质递送到细胞。 生物反应器可用于生长肝组织，并被设计为体外肝辅助装置，其中血液或血浆暴露于三维肝组织。 来自患者的血液或血浆导向流向肝组织。 肝脏组织进一步暴露在与提供营养和气体的介质的相对侧。 该装置在血液或血浆，组织和培养基之间提供多孔边界，允许营养和蛋白质通过扩散梯度递送以透析患者的血液。

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