公开(公告)号：US5559022A

公开(公告)日：1996-09-24

申请号：US378762

申请日：1995-01-26

Applicant: Brian A. Naughton ,  Benson Sibanda

Inventor： Brian A. Naughton ,  Benson Sibanda

IPC: A61K35/12 ,  A61K35/407 ,  A61K48/00 ,  A61P1/16 ,  C12N5/074 ,  C12N5/22 ,  C12N5/06 ,  C12N5/08

CPC classification number: A61K48/00 ,  A61K35/407 ,  C12N5/0672 ,  A61K35/12

Abstract: The present invention relates to liver reserve or progenitor cells. In particular, it relates to the isolation, characterization, culturing, and uses of liver reserve cells. Liver reserve cells isolated by density gradient centrifugation can be distinguished from other liver parenchymal cells by their morphology, staining characteristics, high proliferative activity and ability to differentiate in vitro. In long-term cultures described herein, these cells expand in numbers and differentiate into morphologically mature liver parenchymal cells, capable of mediating liver-specific functions. Therefore, isolated liver reserve cells may have a wide range of applications, including, but not limited to, their uses as vehicles of exogenous genes in gene therapy, and/or to replace and reconstitute a destroyed, infected, or genetically deficient mammalian liver by transplantation.

Abstract translation: 本发明涉及肝脏储备或祖细胞。 特别涉及肝储备细胞的分离，表征，培养和使用。 通过密度梯度离心分离的肝储备细胞可以通过其形态，染色特征，高增殖活性和体外分化能力与其他肝实质细胞区分开来。 在本文所述的长期培养物中，这些细胞数量增多，分化成形态学上成熟的肝实质细胞，能够介导肝脏特异性功能。 因此，分离的肝储备细胞可以具有广泛的应用，包括但不限于它们作为基因治疗中外源基因的载体的用途，和/或通过以下方式替代和重构被破坏的，感染的或基因缺陷的哺乳动物肝脏： 移植。

公开(公告)号：US5827729A

公开(公告)日：1998-10-27

申请号：US636591

申请日：1996-04-23

Applicant: Brian A. Naughton ,  Craig R. Halberstadt ,  Benson Sibanda

Inventor： Brian A. Naughton ,  Craig R. Halberstadt ,  Benson Sibanda

IPC: C12M1/00 ,  A01N1/02 ,  A61F2/02 ,  A61M1/34 ,  A61M1/36 ,  C12M1/16 ,  C12M3/06 ,  C12N5/00 ,  C12N5/071 ,  B01D63/00

CPC classification number: C12M29/04 ,  A61F2/022 ,  A61M1/3489 ,  C12M21/08 ,  C12M25/14 ,  C12M35/04 ,  C12M35/08 ,  C12M41/32 ,  C12N5/0671 ,  C12N2500/02 ,  C12N2500/32

Abstract: A tissue engineering bioreactor is disclosed for growing three-dimensional tissue. Cells are seeded onto a mesh and provided with two media flows, each contacting a different side of the cells. The media flows contain different concentrations of nutrients, allowing nutrients to be delivered to the cells by diffusion gradient. The bioreactor can be used to grow liver tissue, and designed as an extracorporeal liver assist device in which blood or plasma is exposed to the three-dimensional liver tissue. The blood or plasma from a patient directed to flow against the liver tissue. The liver tissue is further exposed on its opposite side to media providing nutrients and gases. The device provides porous boundaries between the blood or plasma, tissue, and media, allowing nutrient and protein delivery by diffusion gradient to dialyze a patient's blood.

Abstract translation: 公开了用于生长三维组织的组织工程生物反应器。 将细胞接种到网状物上，并提供两个培养基流，每个接触细胞的不同侧。 培养基流中含有不同浓度的营养物质，允许通过扩散梯度将营养物质递送到细胞。 生物反应器可用于生长肝组织，并被设计为体外肝辅助装置，其中血液或血浆暴露于三维肝组织。 来自患者的血液或血浆导向流向肝组织。 肝脏组织进一步暴露在与提供营养和气体的介质的相对侧。 该装置在血液或血浆，组织和培养基之间提供多孔边界，允许营养和蛋白质通过扩散梯度递送以透析患者的血液。

公开(公告)号：US06218182B1

公开(公告)日：2001-04-17

申请号：US09470317

申请日：1999-12-21

Applicant: Brian A. Naughton ,  Craig R. Halberstadt ,  Benson Sibanda

Inventor： Brian A. Naughton ,  Craig R. Halberstadt ,  Benson Sibanda

IPC: A01N102

CPC classification number: C12M29/04 ,  A61F2/022 ,  A61M1/3489 ,  C12M21/08 ,  C12M25/14 ,  C12M35/04 ,  C12M35/08 ,  C12M41/32 ,  C12N5/0671 ,  C12N2500/02 ,  C12N2500/32

Abstract: A tissue engineering bioreactor is disclosed for growing three-dimensional tissue. Cells are seeded onto a mesh and provided with two media flows, each contacting a different side of the cells. The media flows contain different concentrations of nutrients, allowing nutrients to be delivered to the cells by diffusion gradient. The bioreactor can be used to grow liver tissue, and designed as an extracorporeal liver assist device in which blood or plasma is exposed to the three-dimensional liver tissue. The blood or plasma from a patient directed to flow against the liver tissue. The liver tissue is further exposed on its opposite side to media providing nutrients and gases. The device provides porous boundaries between the blood or plasma, tissue, and media; allowing nutrient and protein delivery by diffusion gradient to dialyze a patient's blood.

Abstract translation: 公开了用于生长三维组织的组织工程生物反应器。 将细胞接种到网状物上，并提供两个培养基流，每个接触细胞的不同侧。 培养基流中含有不同浓度的营养物质，允许通过扩散梯度将营养物质递送到细胞。 生物反应器可用于生长肝组织，并被设计为体外肝辅助装置，其中血液或血浆暴露于三维肝组织。 来自患者的血液或血浆导向流向肝组织。 肝脏组织进一步暴露在与提供营养和气体的介质的相对侧。 该装置在血液或血浆，组织和培养基之间提供多孔边界; 允许营养和蛋白质通过扩散梯度递送以透析患者的血液。

公开(公告)号：US6008049A

公开(公告)日：1999-12-28

申请号：US136503

申请日：1998-08-19

Applicant: Brian A. Naughton ,  Craig R. Halberstadt ,  Benson Sibanda

Inventor： Brian A. Naughton ,  Craig R. Halberstadt ,  Benson Sibanda

IPC: C12M1/00 ,  A01N1/02 ,  A61F2/02 ,  A61M1/34 ,  A61M1/36 ,  C12M1/16 ,  C12M3/06 ,  C12N5/00 ,  C12N5/071 ,  C12M3/00

CPC classification number: C12M29/04 ,  A61F2/022 ,  A61M1/3489 ,  C12M21/08 ,  C12M25/14 ,  C12M35/04 ,  C12M35/08 ,  C12M41/32 ,  C12N5/0671 ,  C12N2500/02 ,  C12N2500/32

Abstract: A tissue engineering bioreactor is disclosed for growing three-dimensional tissue. Cells are seeded onto a mesh and provided with two media flows, each contacting a different side of the cells. The media flows contain different concentrations of nutrients, allowing nutrients to be delivered to the cells by diffusion gradient. The bioreactor can be used to grow liver tissue, and designed as an extracorporeal liver assist device in which blood or plasma is exposed to the three-dimensional liver tissue. The blood or plasma from a patient directed to flow against the liver tissue. The liver tissue is further exposed on its opposite side to media providing nutrients and gases. The device provides porous boundaries between the blood or plasma, tissue, and media, allowing nutrient and protein delivery by diffusion gradient to dialyze a patient's blood.

Abstract translation: 公开了用于生长三维组织的组织工程生物反应器。 将细胞接种到网状物上，并提供两个培养基流，每个接触细胞的不同侧。 培养基流中含有不同浓度的营养物质，允许通过扩散梯度将营养物质递送到细胞。 生物反应器可用于生长肝组织，并被设计为体外肝辅助装置，其中血液或血浆暴露于三维肝组织。 来自患者的血液或血浆导向流向肝组织。 肝脏组织进一步暴露在与提供营养和气体的介质的相对侧。 该装置在血液或血浆，组织和培养基之间提供多孔边界，允许营养和蛋白质通过扩散梯度递送以透析患者的血液。