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    Enhancement of immunochemical staining in aldehyde-fixed tissue
    6.
    再颁专利
    Enhancement of immunochemical staining in aldehyde-fixed tissue 失效
    增强醛固定组织中的免疫化学染色

    公开(公告)号：USRE40835E1

    公开(公告)日：2009-07-07

    申请号：US11249180

    申请日：1993-08-12

    申请人： Shan-Rong Shi ,  Atul K. Tandon ,  Krishan L. Kalra ,  Nagesh Malhotra ,  Sheng-Hui Su ,  Cheng-Zhi Yu

    发明人： Shan-Rong Shi ,  Atul K. Tandon ,  Krishan L. Kalra ,  Nagesh Malhotra ,  Sheng-Hui Su ,  Cheng-Zhi Yu

    IPC分类号： G01N33/567

    CPC分类号： G01N1/30 ,  G01N33/5306 ,  Y10T436/25125

    摘要： A method for restoring immunoreactivity of a tissue, particularly decalcified tissue, fixed width an aldehyde fixative agent and embedded in an embedding medium, usually comprising celloidin, the method comprising the steps of contacting the tissue with an aldehyde releasing reagent solution comprising a solvent and an aldehyde releasing reagent and removing aldehyde released by the aldehyde releasing reagent from contact with the tissue by reacting the aldehyde in a substantially irreversible manner to form a non-aldehyde derivative, and removing excess base from the tissue. A preferred solution for celloidin-embedded decalcified tissue comprises methanolic sodium hydroxide at about one-third saturation.

    摘要翻译： 一种恢复组织，特别是脱钙组织的免疫反应性的方法，固定宽度的醛固定剂并包埋在通常包含肌内注射的包埋培养基中，所述方法包括以下步骤：使组织与包含溶剂的醛释放试剂溶液接触， 醛释放试剂和通过使醛释放试剂释放的醛与基本上不可逆的方式反应而除去与组织接触的醛，以形成非醛衍生物，并从组织中除去多余的碱。 对于胶原蛋白包埋的脱钙组织的优选溶液包含约三分之一饱和的甲醇氢氧化钠。

    Deparaffinization compositions for dewaxing tissue specimens
    10.
    发明申请
    Deparaffinization compositions for dewaxing tissue specimens 审中-公开
    用于脱蜡组织标本的脱蜡组合物

    公开(公告)号：US20060019332A1

    公开(公告)日：2006-01-26

    申请号：US11186392

    申请日：2005-07-20

    申请人： Guangrong Zhang ,  Cheng-Zhi Yu ,  Sheng-Hui Su ,  Krishan Kalra ,  Ding Zhou

    发明人： Guangrong Zhang ,  Cheng-Zhi Yu ,  Sheng-Hui Su ,  Krishan Kalra ,  Ding Zhou

    IPC分类号： G01N1/30 ,  G01N33/48 ,  C23G5/00

    CPC分类号： C11D1/143 ,  C11D1/62 ,  C11D1/72 ,  C11D3/187 ,  C11D3/188 ,  C11D3/201 ,  C11D3/2044 ,  G01N1/30 ,  G01N1/36 ,  Y10S435/975

    摘要： Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual dewaxing composition. Also provided is a kit for dewaxing a wax-embedded specimen, which comprises a dewaxing composition and can further comprise a second composition of (1) an immunostaining reagent or (2) an aqueous wash solution comprising a detergent for removing residual dewaxing solution.

    摘要翻译： 提供组合物和方法，用于在组织化学分析前对蜡嵌入的生物样品进行脱蜡。 所提供的组合物和方法可以在用于组织化学或其他诊断分析的准备期间有效地从标本中去除蜡或改进的蜡基嵌入材料，特别是基于石蜡的包埋材料，同时最小化对使用者的危害，实现与自动使用的兼容性并保持与下游的兼容性 组织化学分析，特别是免疫染色。 本发明的组合物包含石蜡溶解性有机溶剂，极性有机溶剂和表面活性剂。 组合物还可以包含水。 该方法包括使蜡包埋的样品与脱蜡组合物接触，以在组织化学分析之前溶解浸渍样品的蜡。 该方法可以包括在用包含洗涤剂的水性洗涤组合物脱蜡之后立即洗涤脱蜡样品以除去残余脱蜡组合物的步骤。 还提供了一种用于脱蜡蜡包埋样品的试剂盒，其包含脱蜡组合物，并且还可以包含（1）免疫染色试剂的第二组合物或（2）包含用于除去残余脱蜡溶液的洗涤剂的水性洗涤溶液。