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公开(公告)号：US07070951B2
公开(公告)日：2006-07-04
申请号：US10652854
申请日：2003-08-29
申请人： Guangrong Zhang , Cheng-Zhi Yu , Sheng-Hui Su , Krishan L. Kalra , Ding Zhou
发明人： Guangrong Zhang , Cheng-Zhi Yu , Sheng-Hui Su , Krishan L. Kalra , Ding Zhou
IPC分类号： G01N1/30
CPC分类号： C11D1/143 , C11D1/62 , C11D1/72 , C11D3/187 , C11D3/188 , C11D3/201 , C11D3/2044 , G01N1/30 , G01N1/36 , Y10S435/975
摘要： Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual dewaxing composition. Also provided is a kit for dewaxing a wax-embedded specimen, which comprises a dewaxing composition and can further comprise a second composition of (1) an immunostaining reagent or (2) an aqueous wash solution comprising a detergent for removing residual dewaxing solution.
摘要翻译： 提供组合物和方法，用于在组织化学分析前对蜡嵌入的生物样品进行脱蜡。 所提供的组合物和方法可以在用于组织化学或其他诊断分析的准备期间有效地从标本中去除蜡或改进的蜡基嵌入材料，特别是基于石蜡的包埋材料，同时最小化对使用者的危害，实现与自动使用的兼容性并保持与下游的兼容性 组织化学分析，特别是免疫染色。 本发明的组合物包含石蜡溶解性有机溶剂，极性有机溶剂和表面活性剂。 组合物还可以包含水。 该方法包括使蜡包埋的样品与脱蜡组合物接触，以在组织化学分析之前溶解浸渍样品的蜡。 该方法可以包括在用包含洗涤剂的水性洗涤组合物脱蜡之后立即洗涤脱蜡样品以除去残余脱蜡组合物的步骤。 还提供了一种用于脱蜡蜡包埋样品的试剂盒，其包含脱蜡组合物，并且还可以包含（1）免疫染色试剂的第二组合物或（2）包含用于除去残余脱蜡溶液的洗涤剂的水性洗涤溶液。
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公开(公告)号：US06426195B1
公开(公告)日：2002-07-30
申请号：US09538849
申请日：2000-03-28
申请人： Shude Zhong , Taiying Chen , Sunil Kumar Aggarwal , Sheng-Hui Su
发明人： Shude Zhong , Taiying Chen , Sunil Kumar Aggarwal , Sheng-Hui Su
IPC分类号： C12Q154
CPC分类号： G01N1/30 , G01N33/6836 , G01N2001/302 , Y10S435/81 , Y10S435/975
摘要： A method of silver staining a pathologic sample is disclosed. The method involves the reduction of silver diamine complexes bound to components of the sample by a reducing agent released from a reducing agent carrier. The use of a reducing agent carrier which slowly releases the reducing agent during the reaction permits staining at ambient temperatures.
摘要翻译： 公开了病理样品的银染方法。 该方法包括通过从还原剂载体释放的还原剂还原与样品组分结合的银二胺络合物。 在反应期间缓慢释放还原剂的还原剂载体的使用允许在环境温度下染色。
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公开(公告)号：US5578452A
公开(公告)日：1996-11-26
申请号：US211595
申请日：1994-04-07
申请人： Shan-Rong Shi , Atul K. Tandon , Krishan L. Kalra , Nagesh Malhotra , Sheng-Hui Su , Cheng-Zhi Yu
发明人： Shan-Rong Shi , Atul K. Tandon , Krishan L. Kalra , Nagesh Malhotra , Sheng-Hui Su , Cheng-Zhi Yu
IPC分类号： G01N1/30 , G01N33/53 , G01N33/567
CPC分类号： G01N1/30 , G01N33/5306 , Y10T436/25125
摘要： A method for restoring immunoreactivity of a tissue, particularly decalcified tissue, fixed width an aldehyde fixative agent and embedded in an embedding medium, usually comprising celloidin, the method comprising the steps of contacting the tissue with an aldehyde releasing reagent solution comprising a solvent and an aldehyde releasing reagent and removing aldehyde released by the aldehyde releasing reagent from contact with the tissue by reacting the aldehyde in a substantially irreversible manner to form a non-aldehyde derivative, and removing excess base from the tissue. A preferred solution for celloidin-embedded decalcified tissue comprises methanolic sodium hydroxide at about one-third saturation.
摘要翻译： PCT No.PCT / US93 / 07550 Sec。 371日期1994年4月7日 102（e）1994年4月7日PCT 1993年8月12日PCT PCT。 公开号WO94 / 04906 日期1994年3月3日一种恢复组织，特别是脱钙组织的免疫反应性的方法，固定宽度的醛固定剂并包埋在通常包含肌内注射的包埋培养基中，该方法包括以下步骤：使组织与醛释放试剂溶液 包括溶剂和醛释放试剂，并且通过使醛以基本上不可逆的方式反应形成非醛衍生物，并从组织中除去多余的碱，除去由醛释放试剂释放的醛与组织的接触。 对于胶原蛋白包埋的脱钙组织的优选溶液包含约三分之一饱和的甲醇氢氧化钠。
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公开(公告)号：US5916750A
公开(公告)日：1999-06-29
申请号：US780725
申请日：1997-01-08
IPC分类号： C12N15/09 , C07D251/34 , C07D495/04 , C07F9/6521 , C07H21/00 , C07H21/02 , C07H21/04 , C12Q1/68 , G01N33/543 , C07D413/00
CPC分类号： C07H21/00
摘要： Reagents capable of forming branched oligomers with monomeric units are disclosed, together with oligomers incorporating such reagents, kits containing such reagents and methods for use of such reagents in forming oligomers with monomeric units. The present reagents can advantageously be used to introduce multiple labels or reporter molecules onto oligomers such as oligonucleotides and oligopeptides. In particular, non-nucleosidic phosphoramidites based on 1,3,5-tris(2-hydroxyethyl)cyanuric acid are disclosed. Multiply-labeled, branched DNA oligomer probes constructed using these phosphoramidite reagents showed increased signal intensity relative to singly-labeled oligomer probes.
摘要翻译： 公开了能够形成具有单体单元的支链低聚物的试剂，连同含有此类试剂的低聚物，含有这些试剂的试剂盒和用于形成具有单体单元的低聚物的这种试剂的方法。 本发明的试剂可有利地用于将多个标记或报告分子引入低聚物如寡核苷酸和寡肽。 特别地，公开了基于1,3,5-三（2-羟乙基）氰尿酸的非核苷亚磷酰胺。 使用这些亚磷酰胺试剂构建的多重标记的支链DNA寡聚体探针相对于单标记的寡聚体探针显示增加的信号强度。
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公开(公告)号：USRE40835E1
公开(公告)日：2009-07-07
申请号：US11249180
申请日：1993-08-12
申请人： Shan-Rong Shi , Atul K. Tandon , Krishan L. Kalra , Nagesh Malhotra , Sheng-Hui Su , Cheng-Zhi Yu
发明人： Shan-Rong Shi , Atul K. Tandon , Krishan L. Kalra , Nagesh Malhotra , Sheng-Hui Su , Cheng-Zhi Yu
IPC分类号： G01N33/567
CPC分类号： G01N1/30 , G01N33/5306 , Y10T436/25125
摘要： A method for restoring immunoreactivity of a tissue, particularly decalcified tissue, fixed width an aldehyde fixative agent and embedded in an embedding medium, usually comprising celloidin, the method comprising the steps of contacting the tissue with an aldehyde releasing reagent solution comprising a solvent and an aldehyde releasing reagent and removing aldehyde released by the aldehyde releasing reagent from contact with the tissue by reacting the aldehyde in a substantially irreversible manner to form a non-aldehyde derivative, and removing excess base from the tissue. A preferred solution for celloidin-embedded decalcified tissue comprises methanolic sodium hydroxide at about one-third saturation.
摘要翻译： 一种恢复组织，特别是脱钙组织的免疫反应性的方法，固定宽度的醛固定剂并包埋在通常包含肌内注射的包埋培养基中，所述方法包括以下步骤：使组织与包含溶剂的醛释放试剂溶液接触， 醛释放试剂和通过使醛释放试剂释放的醛与基本上不可逆的方式反应而除去与组织接触的醛，以形成非醛衍生物，并从组织中除去多余的碱。 对于胶原蛋白包埋的脱钙组织的优选溶液包含约三分之一饱和的甲醇氢氧化钠。
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公开(公告)号：US20060019332A1
公开(公告)日：2006-01-26
申请号：US11186392
申请日：2005-07-20
申请人： Guangrong Zhang , Cheng-Zhi Yu , Sheng-Hui Su , Krishan Kalra , Ding Zhou
发明人： Guangrong Zhang , Cheng-Zhi Yu , Sheng-Hui Su , Krishan Kalra , Ding Zhou
CPC分类号： C11D1/143 , C11D1/62 , C11D1/72 , C11D3/187 , C11D3/188 , C11D3/201 , C11D3/2044 , G01N1/30 , G01N1/36 , Y10S435/975
摘要： Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual dewaxing composition. Also provided is a kit for dewaxing a wax-embedded specimen, which comprises a dewaxing composition and can further comprise a second composition of (1) an immunostaining reagent or (2) an aqueous wash solution comprising a detergent for removing residual dewaxing solution.
摘要翻译： 提供组合物和方法，用于在组织化学分析前对蜡嵌入的生物样品进行脱蜡。 所提供的组合物和方法可以在用于组织化学或其他诊断分析的准备期间有效地从标本中去除蜡或改进的蜡基嵌入材料，特别是基于石蜡的包埋材料，同时最小化对使用者的危害，实现与自动使用的兼容性并保持与下游的兼容性 组织化学分析，特别是免疫染色。 本发明的组合物包含石蜡溶解性有机溶剂，极性有机溶剂和表面活性剂。 组合物还可以包含水。 该方法包括使蜡包埋的样品与脱蜡组合物接触，以在组织化学分析之前溶解浸渍样品的蜡。 该方法可以包括在用包含洗涤剂的水性洗涤组合物脱蜡之后立即洗涤脱蜡样品以除去残余脱蜡组合物的步骤。 还提供了一种用于脱蜡蜡包埋样品的试剂盒，其包含脱蜡组合物，并且还可以包含（1）免疫染色试剂的第二组合物或（2）包含用于除去残余脱蜡溶液的洗涤剂的水性洗涤溶液。
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公开(公告)号：US06632598B1
公开(公告)日：2003-10-14
申请号：US08212175
申请日：1994-03-11
申请人： Guangrong Zhang , Cheng-Zhi Yu , Sheng-Hui Su , Krishan L. Kalra , Ding Zhou
发明人： Guangrong Zhang , Cheng-Zhi Yu , Sheng-Hui Su , Krishan L. Kalra , Ding Zhou
IPC分类号： C12Q100
CPC分类号： C11D1/143 , C11D1/62 , C11D1/72 , C11D3/187 , C11D3/188 , C11D3/201 , C11D3/2044 , G01N1/30 , G01N1/36 , Y10S435/975
摘要： Compositions and methods are provided for dewaxing wax-embedded biological specimens prior to histochemical analysis. The compositions and methods provided can effectively remove wax or improved wax-based embedding materials, particularly paraffin-based, from specimens during preparation for histochemical or other diagnostic analyses, while minimizing danger to users, achieving compatibility with automated use, and maintaining compatibility with downstream histochemical analyses, particularly immunostaining. Compositions of the invention comprise a paraffin-solubilizing organic solvent, a polar organic solvent, and a surfactant. Compositions can further comprise water. The method involves contacting a wax-embedded specimen with the dewaxing composition to solubilize the wax impregnating the specimen prior to histochemical analysis. The method can comprise the further step of washing the dewaxed specimen immediately after dewaxing with an aqueous wash composition comprising a detergent to remove residual dewaxing composition. Also provided is a kit for dewaxing a wax-embedded specimen, which comprises a dewaxing composition and can further comprise a second composition of (1) an immunostaining reagent or (2) an aqueous wash solution comprising a detergent for removing residual dewaxing solution.
摘要翻译： 提供组合物和方法，用于在组织化学分析前对蜡嵌入的生物样品进行脱蜡。 所提供的组合物和方法可以在用于组织化学或其他诊断分析的准备期间有效地从标本中去除蜡或改进的蜡基嵌入材料，特别是基于石蜡的包埋材料，同时最小化对使用者的危害，实现与自动使用的兼容性并保持与下游的兼容性 组织化学分析，特别是免疫染色。 本发明的组合物包含石蜡溶解性有机溶剂，极性有机溶剂和表面活性剂。 组合物还可以包含水。 该方法包括使蜡包埋的样品与脱蜡组合物接触，以在组织化学分析之前溶解浸渍样品的蜡。 该方法可以包括在用包含洗涤剂的水性洗涤组合物脱蜡之后立即洗涤脱蜡样品以除去残余脱蜡组合物的步骤。 还提供了一种用于脱蜡蜡包埋样品的试剂盒，其包含脱蜡组合物，并且还可以包含第二组合物（1）免疫染色试剂或（2）包含用于除去残余脱蜡溶液的洗涤剂的水性洗涤溶液。 / PTEXT>
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公开(公告)号：US06451551B1
公开(公告)日：2002-09-17
申请号：US09573073
申请日：2000-05-16
申请人： Guangrong Zhan , Krishan L. Kalra , Sheng-Hui Su , Taiying Chen
发明人： Guangrong Zhan , Krishan L. Kalra , Sheng-Hui Su , Taiying Chen
IPC分类号： G01N130
摘要： Releasing the embedding medium from an embedded histochemically reactive tissue specimen is provided by contacting the embedded tissue specimen with a releasing composition under conditions sufficient to release a sufficient portion of the embedding medium associated with the histochemically reactive tissue specimen to permit analysis without substantial adverse effect on the histochemical reactivity of the specimen.
摘要翻译： 通过使嵌入的组织样本与释放组合物在足以释放与组织化学反应性组织标本相关的足够部分的包埋培养基的条件下接触来提供嵌入介质，以允许分析，而不会对 样品的组织化学反应性。
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公开(公告)号：US6130323A
公开(公告)日：2000-10-10
申请号：US171925
申请日：1998-10-26
CPC分类号： C07H21/00 , C07F9/2416 , C07F9/6561
摘要： Non-nucleotide reagents capable of forming oligomers with nucleotide units are disclosed, together with intermediates for synthesizing such non-nucleotide reagents, oligomers incorporating such reagents, kits containing such reagents and methods for their use in forming oligomers with nucleotide units.
摘要翻译： PCT No.PCT / US97 / 09094 Sec。 371日期：1998年10月26日 102（e）日期1998年10月26日PCT提交1997年5月15日PCT公布。 第WO97 / 43451号公报 日本1997年11月20日公开了能够形成具有核苷酸单元的寡聚体的非核苷酸试剂，以及用于合成这种非核苷酸试剂的中间体，含有此类试剂的低聚物，含有此类试剂的试剂盒及其用于形成具有核苷酸单元的寡聚体 。
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