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11 条记录 (耗时 0.022 秒)

    Polynucleotide constructs
    1.
    发明授权
    Polynucleotide constructs 有权
    多核苷酸构建体

    公开(公告)号：US07754944B2

    公开(公告)日：2010-07-13

    申请号：US11695108

    申请日：2007-04-02

    申请人： Changlin Fu ,  Brian Hauge ,  Vicky Gavrias

    发明人： Changlin Fu ,  Brian Hauge ,  Vicky Gavrias

    IPC分类号： C12N15/84 ,  C12N15/65 ,  C12N15/66 ,  C12N5/04 ,  C12N5/10 ,  A01H5/00 ,  A01H5/10

    CPC分类号： C12N15/8273 ,  C07K14/415 ,  C12N9/1205

    摘要： Synthetic modular polynucleotide constructs are useful for gene expression in plants, methods for making and using such constructs, and plants transformed with such constructs. The constructs comprise unique restriction sites for each modular element and may further comprise polynucleotide identifier sequences.

    摘要翻译： 合成的模块化多核苷酸构建体可用于植物中的基因表达，用于制备和使用这些构建体的方法，以及用这些构建体转化的植物。 构建体包含用于每个模块化元件的独特的限制性位点，并且还可以包含多核苷酸标识符序列。

    Non-random method of gene shuffling
    2.
    发明申请
    Non-random method of gene shuffling 审中-公开
    基因改组的非随机方法

    公开(公告)号：US20060141626A1

    公开(公告)日：2006-06-29

    申请号：US11258833

    申请日：2005-10-26

    申请人： Brian Hauge ,  Fenggao Dong

    发明人： Brian Hauge ,  Fenggao Dong

    IPC分类号： C12N15/87

    CPC分类号： C12N15/1031 ,  C12N15/1027 ,  C12N15/1093 ,  C12N15/64 ,  C12N15/66

    摘要： The present invention concerns the non-random assembling of DNA molecules in a DNA construct and methods of using such constructs, including the production of nucleic acid libraries. The non-random gene shuffling is preferably accomplished by the following steps. First, optionally, the amino acid sequences of proteins encoded by related gene families of interest are aligned and inspected for regions of conserved amino acid residues. These conserved regions, preferably of at least 4 (e.g. about 4 to 10) consecutive conserved amino acid residues are candidate regions for the subsequent design of PCR primers to amplify the variable or less conserved regions in between them, followed by non-random reassembly to create a recombinant nucleic acid genetic library of gene family variants.

    摘要翻译： 本发明涉及DNA构建体中DNA分子的非随机组装以及使用这些构建体的方法，包括产生核酸文库。 非随机基因改组优选通过以下步骤完成。 首先，任选地，由感兴趣的相关基因家族编码的蛋白质的氨基酸序列进行比对并检查保守氨基酸残基的区域。 这些保守区域，优选至少4个（例如约4至10个）连续的保守氨基酸残基是候选区域，用于后续设计的PCR引物以扩增它们之间的可变区或较少保守区域，随后是非随机重组 创建基因家族变体的重组核酸基因文库。

    Methods for ligation independent cloning of DNA
    3.
    发明申请
    Methods for ligation independent cloning of DNA 有权

    公开(公告)号：US20060147961A1

    公开(公告)日：2006-07-06

    申请号：US11298234

    申请日：2005-12-09

    申请人： Fenggao Dong ,  Brian Hauge ,  Christopher Oggero

    发明人： Fenggao Dong ,  Brian Hauge ,  Christopher Oggero

    IPC分类号： C12Q1/68 ,  C12P19/34

    CPC分类号： C12N15/66 ,  C12N15/64

    摘要： The present invention provides methods for assembling DNA molecules in a predetermined order to produce a DNA construct useful in Agrobacterium mediated transformation in plants. The method employs ligation independent cloning of separate DNA elements where one of the DNA elements contains T-DNA borders from Agrobacterium tumefaciens. The invention further provides methods for assembling a construct containing an inverted repeat. Using this approach, DNA constructs are constructed rapidly, efficiently and directionally.

    Right angle adaptor
    4.
    发明授权
    Right angle adaptor 有权
    直角适配器

    公开(公告)号：US08628356B2

    公开(公告)日：2014-01-14

    申请号：US13503516

    申请日：2010-10-22

    申请人： John C. Laurx ,  Peerouz Amleshi ,  Brian Hauge

    发明人： John C. Laurx ,  Peerouz Amleshi ,  Brian Hauge

    IPC分类号： H01R31/06

    CPC分类号： H01R31/06 ,  H01R12/737 ,  H01R13/6471 ,  H01R2107/00

    摘要： An adaptor includes a first and second recess that face in opposing directions and that are configured to receive a first and second connector. A floor in the adaptor can separate the first recess from the second recess. A pin array can be positioned in the floor and the pin array can extend in two directions from the floor so as to extend into the first and second recess. The pin array can include terminals that are configured with first and second contact ends that are respectively positioned in the first and second recess. The first and second contact ends can be respectively configured with a first and second orientation that are at a right angle with respect to each other.

    摘要翻译： 适配器包括面向相反方向并且被配置为接收第一和第二连接器的第一和第二凹部。 适配器中的地板可以将第一凹部与第二凹部分开。 针阵列可以定位在地板中，并且销阵列可以从地板沿两个方向延伸，以便延伸到第一和第二凹部中。 引脚阵列可以包括被配置有分别位于第一和第二凹部中的第一和第二接触端的端子。 第一和第二接触端可以分别配置有相对于彼此成直角的第一和第二取向。

    RIGHT ANGLE ADAPTOR
    5.
    发明申请
    RIGHT ANGLE ADAPTOR 有权
    右角适配器

    公开(公告)号：US20120264334A1

    公开(公告)日：2012-10-18

    申请号：US13503516

    申请日：2010-10-22

    申请人： John C. Laurx ,  Peerouz Amleshi ,  Brian Hauge

    发明人： John C. Laurx ,  Peerouz Amleshi ,  Brian Hauge

    IPC分类号： H01R31/06

    CPC分类号： H01R31/06 ,  H01R12/737 ,  H01R13/6471 ,  H01R2107/00

    摘要： An adaptor includes a first and second recess that face in opposing directions and that are configured to receive a first and second connector. A floor in the adaptor can separate the first recess from the second recess. A pin array can be positioned in the floor and the pin array can extend in two directions from the floor so as to extend into the first and second recess. The pin array can include terminals that are configured with first and second contact ends that are respectively positioned in the first and second recess. The first and second contact ends can be respectively configured with a first and second orientation that are at a right angle with respect to each other.

    摘要翻译： 适配器包括面向相反方向并且被配置为接收第一和第二连接器的第一和第二凹部。 适配器中的地板可以将第一凹部与第二凹部分开。 针阵列可以定位在地板中，并且销阵列可以从地板沿两个方向延伸，以便延伸到第一和第二凹部中。 引脚阵列可以包括被配置有分别位于第一和第二凹部中的第一和第二接触端的端子。 第一和第二接触端可以分别配置有相对于彼此成直角的第一和第二取向。

    Transgenic Plants with Enhanced Agronomic Traits
    6.
    发明申请
    Transgenic Plants with Enhanced Agronomic Traits 审中-公开
    具有增强农艺性状的转基因植物

    公开(公告)号：US20110247094A1

    公开(公告)日：2011-10-06

    申请号：US13122581

    申请日：2009-10-07

    申请人： Edward Allen ,  Molian Deng ,  Brian Hauge ,  Sergey Ivashuta ,  Huai Wang

    发明人： Edward Allen ,  Molian Deng ,  Brian Hauge ,  Sergey Ivashuta ,  Huai Wang

    IPC分类号： A01H1/02 ,  C12N15/82 ,  A01H5/00 ,  C12N5/10 ,  A01H5/10 ,  C12Q1/68

    CPC分类号： C12N15/8218 ,  C07K14/415 ,  C12N15/8247 ,  C12N15/8251 ,  C12N15/8261 ,  C12N15/8271 ,  C12N15/8273 ,  C12N15/8274 ,  C12N15/8275 ,  Y02A40/146

    摘要： This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.

    摘要翻译： 本发明提供具有用于表达蛋白质的重组DNA的转基因植物细胞，其可用于赋予转基因作物植物增强的农艺性状。 本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐寒性，增加的产量，增强的氮利用效率，增强的氮利用效率， 种子蛋白和增强种子油。 还公开了用于制备具有增强特征的转基因种子和植物的方法。

    Polynucleotide Constructs
    7.
    发明申请
    Polynucleotide Constructs 有权
    多核苷酸构建体

    公开(公告)号：US20070234442A1

    公开(公告)日：2007-10-04

    申请号：US11695108

    申请日：2007-04-02

    申请人： Changlin Fu ,  Brian Hauge ,  Vicky Gavrias

    发明人： Changlin Fu ,  Brian Hauge ,  Vicky Gavrias

    IPC分类号： A01H1/00 ,  C12N15/82 ,  C12N5/04

    CPC分类号： C12N15/8273 ,  C07K14/415 ,  C12N9/1205

    摘要： Synthetic modular polynucleotide constructs are useful for gene expression in plants, methods for making and using such constructs, and plants transformed with such constructs. The constructs comprise unique restriction sites for each modular element and may further comprise polynucleotide identifier sequences.

    摘要翻译： 合成的模块化多核苷酸构建体可用于植物中的基因表达，用于制备和使用这些构建体的方法，以及用这些构建体转化的植物。 构建体包含用于每个模块化元件的独特的限制性位点，并且还可以包含多核苷酸标识符序列。

    Nucleic acid molecules and other molecules associated with soybean cyst nematode resistance
    10.
    发明申请
    Nucleic acid molecules and other molecules associated with soybean cyst nematode resistance 有权

    公开(公告)号：US20060253919A1

    公开(公告)日：2006-11-09

    申请号：US11389017

    申请日：2006-03-27

    申请人： Brian Hauge ,  Ming Wang ,  Jeremy Parsons ,  Laurence Parnell

    发明人： Brian Hauge ,  Ming Wang ,  Jeremy Parsons ,  Laurence Parnell

    IPC分类号： A01H5/00 ,  C12Q1/68 ,  C12N15/82 ,  A01H1/00 ,  C12N5/04

    CPC分类号： C07K14/415

    摘要： The present invention is in the field of soybean genetics. More specifically, the invention relates to nucleic acid molecules from regions the soybean genome, which are associated with soybean cyst nematode resistance. The invention also relates to proteins encoded by such nucleic acid molecules as well as antibodies capable of recognizing these proteins. The invention also relates to nucleic acid markers from regions the soybean genome, which are associated with soybean cyst nematode resistance. Moreover, the invention relates to uses of such molecules, including, transforming soybean cyst nematode resistant soybean with constructs containing nucleic acid molecules from regions the soybean genome, which are associated with soybean cyst nematode resistance. Furthermore, the invention relates to the use of such molecules in a plant breeding program.