公开(公告)号：US11644455B2

公开(公告)日：2023-05-09

申请号：US17643058

申请日：2021-12-07

Applicant: Bonraybio Co., Ltd.

Inventor： Cheng-Teng Hsu ,  Li-Sheng Chang ,  Hsiu-Chin Lee

IPC: G01N33/483 ,  C12N5/076 ,  G01N21/64

CPC classification number: G01N33/4833 ,  C12N5/061 ,  G01N2021/6439

Abstract: Disclosed herein are methods for the detection of the presence of sperm DNA fragmentation in a semen sample. The methods include embedding of sperm cells of the semen sample in a gel, denaturing DNA of the sperm cells, and lysing the nuclear proteins of the sperm cells. The present method includes an ionic surfactant sodium dodycyl sulfate (SDS) and a chaotropic agent urea in the lysis solution for releasing DNA from protamine of chromosome, which significantly reduces the time required for lysis. A kit for detecting sperm DNA fragmentation in a semen sample is also disclosed.

公开(公告)号：US20220196630A1

公开(公告)日：2022-06-23

申请号：US17643058

申请日：2021-12-07

Applicant: Bonraybio Co., Ltd.

Inventor： Cheng-Teng Hsu ,  Li-Sheng Chang ,  Hsiu-Chin Lee

IPC: G01N33/483 ,  C12N5/076

Abstract: Disclosed herein are methods for the detection of the presence of sperm DNA fragmentation in a semen sample. The methods include embedding of sperm cells of the semen sample in a gel, denaturing DNA of the sperm cells, and lysing the nuclear proteins of the sperm cells. The present method includes an ionic surfactant sodium dodycyl sulfate (SDS) and a chaotropic agent urea in the lysis solution for releasing DNA from protamine of chromosome, which significantly reduces the time required for lysis. A kit for detecting sperm DNA fragmentation in a semen sample is also disclosed.

公开(公告)号：US20210293688A1

公开(公告)日：2021-09-23

申请号：US17180024

申请日：2021-02-19

Applicant: BONRAYBIO CO., LTD.

Inventor： CHIH-PIN CHANG ,  HSI-WEN HUANG

IPC: G01N15/06 ,  G01N33/487

Abstract: A validation test piece is for validating a bio-sample detection device, which includes an insert port for insertion of the validation test piece and a detection module. The validation test piece includes a base seat, a test element, a standardized data and a top cover. The base seat includes a seat body and a receiving member disposed on the seat body. The test element is connected to the receiving member, and includes a test region to be detected by the detection module. The standardized data corresponds to a sample number of the test region. The top cover is connected to the base seat for covering the test element.

公开(公告)号：USD858790S1

公开(公告)日：2019-09-03

申请号：US29598215

申请日：2017-03-23

Applicant: Bonraybio Co., Ltd.

Designer： Cheng-Teng Hsu

公开(公告)号：US20220195492A1

公开(公告)日：2022-06-23

申请号：US17643161

申请日：2021-12-07

Applicant: Bonraybio Co., Ltd.

Inventor： Cheng-Teng Hsu ,  Li-Sheng Chang ,  Hsiu-Chin Lee

IPC: C12Q1/68 ,  G01N1/30 ,  G01N1/36 ,  G01N21/64

Abstract: Disclosed herein is a method for the detection of the presence of sperm DNA fragmentation in a semen sample. The method comprises a step of embedding the semen sample containing sperm cells in a gel comprising acrylamide, acrylic acid, methacrylic acid, N-isopropylacrylamide (NIPAM), alginate, or polyethylene glycol (PEG), to obtain a sperm cells-embedded gel. A kit for detecting sperm DNA fragmentation in a semen sample is also disclosed.

公开(公告)号：US11268947B2

公开(公告)日：2022-03-08

申请号：US17034566

申请日：2020-09-28

Applicant: Bonraybio Co., Ltd.

Inventor： Cheng-Teng Hsu ,  Chih-Pin Chang ,  Kuang-Li Huang ,  Yu-Chiao Chi ,  Chia-Wei Chang ,  Chiung-Han Wang

IPC: G02B21/34 ,  G01N33/48 ,  G01N1/28 ,  H04N5/232 ,  H04N5/225 ,  G01N21/17

Abstract: Embodiments include a device for testing biological specimen. The device can include a receiving mechanism to receive a carrier. The carrier may include a holding area. The device includes a camera to capture a plurality of images of the holding area. A processor in the device may utilize the camera module to: adaptively select, based on the plurality of images of the holding area, an analytical algorithm suitable for a motion property that is characteristic of the biological specimen being tested; and perform a set of analytic processes that corresponds to the selected analytic algorithm on the captured imagery to generate an analytic result associated with the biological specimen.

公开(公告)号：USD912273S1

公开(公告)日：2021-03-02

申请号：US29686019

申请日：2019-04-02

Applicant: BONRAYBIO CO., LTD.

Designer： Cheng-Teng Hsu ,  Chih-Pin Chang ,  Hsi-Wen Huang ,  Kuang-Li Huang

公开(公告)号：USD831224S1

公开(公告)日：2018-10-16

申请号：US29598212

申请日：2017-03-23

Applicant: Bonraybio Co., Ltd.

Designer： Cheng-Teng Hsu

公开(公告)号：US09959621B2

公开(公告)日：2018-05-01

申请号：US15603783

申请日：2017-05-24

Applicant: Bonraybio Co., Ltd.

Inventor： Cheng-Teng Hsu ,  Hsuan-Yu Huang ,  Chih-Pin Chang ,  Kuang-Li Huang ,  Yu-Chiao Chi

IPC: G01N15/06 ,  G06T7/00 ,  G01N21/80 ,  G01N15/14 ,  H04N7/18 ,  H04N5/225 ,  G02B21/36 ,  G02B21/14 ,  G02B21/24

CPC classification number: G06T7/0012 ,  A61B5/14507 ,  A61B5/1455 ,  A61B5/43 ,  A61B10/0058 ,  A61K35/00 ,  C12M21/06 ,  C12M31/02 ,  C12M41/06 ,  C12M41/46 ,  C12N5/0612 ,  G01N15/06 ,  G01N15/1429 ,  G01N15/1468 ,  G01N21/80 ,  G01N33/689 ,  G01N2015/0693 ,  G01N2015/1006 ,  G01N2015/1075 ,  G01N2015/1486 ,  G01N2015/1497 ,  G02B21/0008 ,  G02B21/082 ,  G02B21/086 ,  G02B21/14 ,  G02B21/244 ,  G02B21/34 ,  G02B21/361 ,  G02B21/365 ,  G06K9/00147 ,  G06K9/00496 ,  G06K9/00744 ,  G06K9/209 ,  G06K9/228 ,  G06K9/38 ,  H04N5/2257 ,  H04N7/181

Abstract: Embodiments disclose a device for testing biological specimen. The device includes a sample carrier and a detachable cover. The sample carrier includes a specimen holding area. The detachable cover is placed on top of the specimen holding area. The detachable cover includes a magnifying component configured to align with the specimen holding area. The focal length of the magnifying component is from 0.1 mm to 8.5 mm. The magnifying component has a linear magnification ratio of at least 1. Some embodiments further include a multi-camera configuration. These embodiments include a first camera module and a second camera module arranged to capture one or more images of the first holding area and the second holding area, respectively. The processor may perform different analytic processes on the captured images of different holding areas to determine an outcome with regard to the biological specimen.

公开(公告)号：US09958665B2

公开(公告)日：2018-05-01

申请号：US15345061

申请日：2016-11-07

Applicant: Bonraybio Co., Ltd.

Inventor： Cheng-Teng Hsu ,  Hsuan-Yu Huang ,  Chih-Pin Chang ,  Kuang-Li Huang ,  Yu-Chiao Chi

IPC: G02B21/02 ,  G02B21/34 ,  G06T7/00 ,  G06T7/246 ,  G02B21/08 ,  G02B21/36 ,  G02B21/24 ,  G01N21/27 ,  G01N15/06

CPC classification number: G02B21/34 ,  A61B5/14507 ,  A61B5/43 ,  A61B10/0058 ,  A61K35/00 ,  C12N5/0612 ,  G01N15/06 ,  G01N15/1475 ,  G01N21/27 ,  G01N33/689 ,  G01N2015/0693 ,  G01N2015/1006 ,  G01N2015/1486 ,  G01N2015/1497 ,  G02B21/0008 ,  G02B21/082 ,  G02B21/086 ,  G02B21/088 ,  G02B21/241 ,  G02B21/361 ,  G02B21/362 ,  G02B21/365 ,  G06K9/00134 ,  G06K9/00147 ,  G06K9/00496 ,  G06K9/209 ,  G06K9/228 ,  G06K9/32 ,  G06K9/4652 ,  G06T7/0012 ,  G06T7/246 ,  G06T2207/10056 ,  G06T2207/30241 ,  G06T2207/30242

Abstract: Embodiments disclose a device for testing biological specimen. The device includes a sample carrier and a detachable cover. The sample carrier includes a specimen holding area. The detachable cover is placed on top of the specimen holding area. The detachable cover includes a magnifying component configured to align with the specimen holding area. The focal length of the magnifying component is from 0.1 mm to 8.5 mm. The magnifying component has a linear magnification ratio of at least 1.