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公开(公告)号:US20240159733A1
公开(公告)日:2024-05-16
申请号:US18394017
申请日:2023-12-22
发明人: Friedhelm SERWANE , Joachim SPATZ
IPC分类号: G01N33/483 , A61B5/00 , C12N5/00 , C12N5/079 , C12N5/0793 , G01J1/04 , G01J1/42 , G01N21/00 , G01N21/64 , G01N33/50
CPC分类号: G01N33/4833 , A61B5/0071 , C12N5/0062 , C12N5/062 , C12N5/0621 , G01J1/0407 , G01J1/42 , G01N21/00 , G01N21/6458 , G01N33/5058 , G01N33/5082 , C12N2506/45 , C12N2513/00 , C12N2527/00
摘要: A measurement device 100 comprises neuronal, in particular retinal, tissue 110 grown from stem cells, the neuronal tissue 110 having a three-dimensional shape neuronal cells that change an electric potential in cells of the neuronal tissue 110 in response to influences that act on the neuronal cells, and a read-out device 130 that is configured to measure neuronal responses of the neuronal tissue 110 via changes in the electric potential generated by the neuronal cells.
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公开(公告)号:US20230266248A1
公开(公告)日:2023-08-24
申请号:US18024120
申请日:2020-09-16
发明人: Hanieh FATTAHI
CPC分类号: G01N21/65 , G01N21/636 , G02B21/0056 , G02B21/0064 , G02B21/0084 , G02B21/0032 , G01N2021/655
摘要: A microscopic imaging method for creating a microscopic sample image (1A) of a sample (1) comprises the steps of arranging the sample (1) on a sampling crystal (10); irradiating the sample (1) with excitation laser pulses (2, 3) and generating sample response pulses (4) with a sample response field as a result of an interaction of the excitation laser pulses (2, 3) with the sample (1); irradiating the sampling crystal (10) with probe laser pulses (5) being temporally synchronized with the excitation laser pulses (2, 3) and spatially overlapped with the sample response pulses (4) in the sampling crystal (10), wherein the probe laser pulses (5) have a shorter wavelength than the excitation laser pulses (2, 3); detecting the sample response field by electric-field sampling with the sampling crystal (10), using the sample response pulses (4) and the probe laser pulses (5); and calculating the sample image (1A) based on the detected sample response field, wherein the excitation laser pulses (2, 3) have a wavelength in a range from mid-infrared to visible light and the sample response pulses (4) are created by a coherent interaction process induced in the sample (1) and with a fixed phase relationship relative to the excitation laser pulses (2, 3), the sampling crystal (10) is a non-centrosymmetric crystal, the irradiating step is repeated at multiple sample points (1A), wherein at each sample point (1A) the irradiating steps are successively repeated with multiple temporal probe delays of the probe laser pulses (5) relative to the excitation laser pulses (2, 3), at each probe delay, a sum or difference frequency pulse (6) of a sample response pulse (4) and a probe laser pulse (5) is generated, and at each probe delay, a spectral interference pulse (7) is created by a spectral interference of the sum or difference frequency pulse (6) and the current probe laser pulse, the detecting step includes sensing a polarization state of the spectral interference pulse (7) by an ellipsometer device (40) at each probe delay, wherein the local sample response field at the sample point (1A) is derived from the polarization states sensed at all probe delays, and the sample image (1A) is calculated based on the sample response field detected at the sample points (1A). Furthermore, a microscopic imaging apparatus is described.
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公开(公告)号:US11613782B2
公开(公告)日:2023-03-28
申请号:US16980121
申请日:2019-03-13
申请人: UNIVERSITY OF CAPE TOWN , STELLENBOSCH UNIVERSITY , SEATTLE CHILDREN'S HOSPITAL , MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V , UNITED KINGDOM RESEARCH AND INNOVATION
发明人: Sara Suliman , Ethan Greene Thompson , Jayne Suzanne Sutherland , Stefan H. E. Kaufmann , Thomas Jens Scriba , Daniel Edward Zak , Gerhard Walzl
IPC分类号: C12Q1/68 , C12P19/34 , C12Q1/6883
摘要: The invention provides a gene signature for use in determining a likelihood of a latent tuberculosis (TB) infection in a subject transitioning to active TB disease. The gene signature comprises at least SEPT4 and BLK, and optionally also GAS6 and/or CD1C. Expression levels of these genes are detected in a sample from the subject, and the ratios of expression of at least two of the above genes are calculated (e.g. SEPT4:BLK, SEPT4:CD1C, GAS6:BLK and/or GAS6:CD1C). A score is assigned to each ratio, the score being indicative of the likelihood of the latent TB infection transitioning into active TB disease, based on the ratio for the respective gene pair. The subject can be identified as having a latent TB infection that is likely to transition into active TB disease or that is not likely to transition into active TB disease based on the score or on the average of the scores.
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公开(公告)号:US11270232B2
公开(公告)日:2022-03-08
申请号:US16055583
申请日:2018-08-06
摘要: In a method of transporting objects with transport vehicles, a starting point, a destination, a starting time and an arrival time are noted as object coordinates in a space-time. A transport path network of possible transport paths together with average speeds on the possible transport ways is defined. Suitable transport paths of the possible transport paths between the object coordinates of the objects are determined and noted in the space-time. In the space-time, one of the suitable transport paths per object is bundled into driving routes of the transport vehicles, transport capacities and the actual locations of the transport vehicles of the plurality of transport vehicles being considered. The driving routes together with the object coordinates of the associated objects are transmitted to those transport vehicles involved. Then, the objects are transported in that the transport vehicles involved are driven along the transmitted driving routes.
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公开(公告)号:US11227710B2
公开(公告)日:2022-01-18
申请号:US16774496
申请日:2020-01-28
发明人: Garrett Hayes Good
摘要: A magnetic closure device has two complementary magnetic closure elements. Each of the two complementary magnetic closure elements includes an elongated magnet carrier having a single direction of main extension, and a plurality of permanent magnets supported by the magnet carrier in defined positions along the direction of main extension. Each of the permanent magnets is permanently magnetized either longitudinally or diametrically with regard to the direction of main extension. The permanent magnets following to each other in the direction of main extension are arranged in a closure alignment pattern having a magnetic non-repetition length extending over three or more of the permanent magnets. The magnet carrier is bendable in at least one direction orthogonal to the direction of main extension.
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公开(公告)号:US20210363580A1
公开(公告)日:2021-11-25
申请号:US16980121
申请日:2019-03-13
申请人: UNIVERSITY OF CAPE TOWN , STELLENBOSCH UNIVERSITY , SEATTLE CHILDREN'S HOSPITAL DOING BUSINESS AS SEATTLE CHILDREN'S RESEARCH INSTITUTE , MAX-PLANCK-GESELLSCHAFT ZUR FOERDERUNG DER WISSENSCHAFTEN E.V , UNITED KINGDOM RESEARCH AND INNOVATION
发明人: Sara Suliman , Ethan Greene Thompson , Jayne Suzanne Sutherland , Stefan H.E. Kaufmann , Thomas Jens Scriba , Daniel Edward Zak , Gerhard Walzl
IPC分类号: C12Q1/6883
摘要: The invention provides a gene signature for use in determining a likelihood of a latent tuberculosis (TB) infection in a subject transitioning to active TB disease. The gene signature comprises at least SEPT4 and BLK, and optionally also GAS6 and/or CD1C. Expression levels of these genes are detected in a sample from the subject, and the ratios of expression of at least two of the above genes are calculated (e.g. SEPT4:BLK, SEPT4:CD1C, GAS6:BLK and/or GAS6:CD1C). A score is assigned to each ratio, the score being indicative of the likelihood of the latent TB infection transitioning into active TB disease, based on the ratio for the respective gene pair. The subject can be identified as having a latent TB infection that is likely to transition into active TB disease or that is not likely to transition into active TB disease based on the score or on the average of the scores.
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公开(公告)号:US11014147B2
公开(公告)日:2021-05-25
申请号:US15579536
申请日:2016-09-06
摘要: Disclosed is an apparatus having a rotatable wheel with a planar external circumferential surface, which is flat in a direction parallel to the axis of rotation of the wheel, at least one nozzle having a nozzle opening for directing a molten metal onto the circumferential surface and a collection means for collecting solidified fibers of metal formed on the circumferential surface from the molten metal and separated from the circumferential surface by centrifugal force generated by rotation of the wheel. The nozzle has a rectangular cross-section having a width of the nozzle opening in the circumferential direction of rotation of the wheel and a length transverse to the circumferential surface of the wheel which is greater than the width. An apparatus is provided for controlling a gas pressure applied to the liquid metal and delivers it to the circumferential surface of the rotatable wheel.
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公开(公告)号:US10417818B2
公开(公告)日:2019-09-17
申请号:US15626728
申请日:2017-06-19
发明人: Matthew Loper , Naureen Mahmood , Michael Black
摘要: A method for providing a three-dimensional body model which may be applied for an animation, based on a moving body, wherein the method comprises providing a parametric three-dimensional body model, which allows shape and pose variations; applying a standard set of body markers; optimizing the set of body markers by generating an additional set of body markers and applying the same for providing 3D coordinate marker signals for capturing shape and pose of the body and dynamics of soft tissue; and automatically providing an animation by processing the 3D coordinate marker signals in order to provide a personalized three-dimensional body model, based on estimated shape and an estimated pose of the body by means of predicted marker locations.
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公开(公告)号:US10274427B2
公开(公告)日:2019-04-30
申请号:US16069107
申请日:2016-01-13
发明人: Thomas M. Jovin , Nathan P. Cook
IPC分类号: G01N21/64
摘要: An emission lifetime measuring method, in particular for measuring a mean lifetime of electronically excited states of a sample, comprises the steps of illuminating the sample with at least one excitation light pulse, time-resolved detecting an emission response from the sample and creating a temporal detector response function, and calculating the mean lifetime of the electronically excited states on the basis of the detector response function, wherein the at least one excitation light pulse is shaped such that the sample achieves an equilibrium excited steady-state including a linearly increasing or constant number of the electronically excited states, the detector response function has a linear response function section with a constant slope, and the mean lifetime (τ) of the electronically excited states is calculated on the basis of at least one of a time position of the linear response function section relative to a reference time of the at least one excitation light pulse and the slope of the linear response function section. Furthermore, an emission lifetime measuring apparatus (100) is described.
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公开(公告)号:US20180195056A1
公开(公告)日:2018-07-12
申请号:US15525487
申请日:2015-11-10
发明人: Steffen FREY , Dirk GOERLICH
CPC分类号: C12N9/641 , C07K1/22 , C12N9/50 , C12N9/6405
摘要: The present invention belongs to the field of biotechnology. More specifically, the present invention provides a protease, a non-naturally occurring fusion protein comprising a corresponding protease recognition site, expression vectors encoding same, host cells comprising said expression vectors, kit of parts as well as methods applying the protease, fusion protein, and uses thereof, as defined in the claims. The presently disclosed protease/protease recognition site is particularly useful in methods requiring an orthogonal set of proteases, and is suitable for use in both prokaryotic and selected eukaryotic expression systems
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