公开(公告)号：US20230266248A1

公开(公告)日：2023-08-24

申请号：US18024120

申请日：2020-09-16

申请人： Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V.

发明人： Hanieh FATTAHI

IPC分类号： G01N21/65 ,  G01N21/63 ,  G02B21/00

CPC分类号： G01N21/65 ,  G01N21/636 ,  G02B21/0056 ,  G02B21/0064 ,  G02B21/0084 ,  G02B21/0032 ,  G01N2021/655

摘要： A microscopic imaging method for creating a microscopic sample image (1A) of a sample (1) comprises the steps of arranging the sample (1) on a sampling crystal (10); irradiating the sample (1) with excitation laser pulses (2, 3) and generating sample response pulses (4) with a sample response field as a result of an interaction of the excitation laser pulses (2, 3) with the sample (1); irradiating the sampling crystal (10) with probe laser pulses (5) being temporally synchronized with the excitation laser pulses (2, 3) and spatially overlapped with the sample response pulses (4) in the sampling crystal (10), wherein the probe laser pulses (5) have a shorter wavelength than the excitation laser pulses (2, 3); detecting the sample response field by electric-field sampling with the sampling crystal (10), using the sample response pulses (4) and the probe laser pulses (5); and calculating the sample image (1A) based on the detected sample response field, wherein the excitation laser pulses (2, 3) have a wavelength in a range from mid-infrared to visible light and the sample response pulses (4) are created by a coherent interaction process induced in the sample (1) and with a fixed phase relationship relative to the excitation laser pulses (2, 3), the sampling crystal (10) is a non-centrosymmetric crystal, the irradiating step is repeated at multiple sample points (1A), wherein at each sample point (1A) the irradiating steps are successively repeated with multiple temporal probe delays of the probe laser pulses (5) relative to the excitation laser pulses (2, 3), at each probe delay, a sum or difference frequency pulse (6) of a sample response pulse (4) and a probe laser pulse (5) is generated, and at each probe delay, a spectral interference pulse (7) is created by a spectral interference of the sum or difference frequency pulse (6) and the current probe laser pulse, the detecting step includes sensing a polarization state of the spectral interference pulse (7) by an ellipsometer device (40) at each probe delay, wherein the local sample response field at the sample point (1A) is derived from the polarization states sensed at all probe delays, and the sample image (1A) is calculated based on the sample response field detected at the sample points (1A). Furthermore, a microscopic imaging apparatus is described.

公开(公告)号：US20200033259A1

公开(公告)日：2020-01-30

申请号：US16496270

申请日：2017-03-21

申请人： Max-Planck-Gesellschaft zur Foerderung der Wissenschaften e.V. ,  Ludwig-Maximilians-Universitaet Muenchen

发明人： Ferenc KRAUSZ ,  Hanieh FATTAHI ,  Marinus HUBER ,  Ioachim PUPEZA ,  Mihaela ZIGMAN KOHLMAIER

IPC分类号： G01N21/3586 ,  G01N21/45 ,  G01N21/21

摘要： A method of measuring a polarization response of a sample (1), in particular a biological sample, comprises the steps of generating a sequence of excitation waves (2), irradiating the sample (1) with the sequence of excitation waves (2), including an interaction of the excitation waves (2) with the sample (1), so that a sequence of sample waves (3) is generated each including a superposition of a sample main pulse and a sample global molecular fmgerprint (GMF) wave (EGMF(sample)(t)), irradiating a reference sample (1A) with the sequence of excitation waves (2), including an interaction of the excitation waves (2) with the reference sample (1A), so that a sequence of reference waves (3A) is generated each including a superposition of a reference main pulse and a reference GMF wave (EGMF(ref)(t)), optically separating a difference of the sample waves (3) and reference waves (3A) from GMF wave contributions which are common to both of the sample waves (3) and reference waves (3A) in space and/or time, and detecting the difference of the sample waves (3) and the reference waves (3A) and determining a temporal amplitude of differential molecular fmgerprint (dMF) waves (ΔGMF) (4) each comprising the difference of the sample and reference GMF waves. Futhermore, as a spectroscopic apparatus for measuring a polarization response of a sample (1) is described.