Method of purifying native ubiquitin

    公开(公告)号:US11459555B2

    公开(公告)日:2022-10-04

    申请号:US17128550

    申请日:2020-12-21

    Abstract: The present invention relates to a method of purifying native ubiquitin without acid or heat treatment. The method presented here is designed to overcome limitations of acid or heat based ubiquitin purification in two different points of view. First, it decreases a chance of mixing other proteins resistant to acids or heat. Second, it includes no harsh condition, which might denature the ubiquitin. As a result, the purification of native ubiquitin becomes possible. The ubiquitin obtained herein is expected to be used for various purposes in technical fields.

    MODIFIED EXTRACELLULAR MATRIX-BASED HYDROGEL, MANUFACTURING METHOD OF THE SAME AND USE OF THE SAME

    公开(公告)号:US20220218465A1

    公开(公告)日:2022-07-14

    申请号:US17502152

    申请日:2021-10-15

    Abstract: A modified extracellular matrix-based hydrogel according to an example of the present disclosure includes an extracellular matrix-denatured collagen conjugate formed by a Michael addition reaction between an extracellular matrix having an amine group and a denatured collagen into which an ethylenically unsaturated bond functional group is introduced. The modified extracellular matrix-based hydrogel according to the present disclosure exhibits enhanced mechanical properties (e.g., viscoelasticity) compared to the extracellular matrix hydrogel before modification. In addition, it shows a high cell viability when the bioink is prepared by encapsulating cells in a modified extracellular matrix-based hydrogel according to the present disclosure. In addition, when an artificial living tissue for transplantation (for example, artificial corneal tissue) manufactured by 3-D printing bioink according to the present disclosure is transplanted into a damaged cornea, it can be sutured and has a transparency similar to that of the real cornea, and corneal tissue can be reconstructed without other side effects due to its enhanced mechanical properties. Accordingly, the modified extracellular matrix-based hydrogel according to the present disclosure can be applied in tissue engineering fields and related fields requiring improvement in physical properties and is particularly useful as a material for corneal transplants.

    DEVICE AND METHOD OF GENERATING NONLINIEAR WAVEFORM SIGNAL

    公开(公告)号:US20220209755A1

    公开(公告)日:2022-06-30

    申请号:US17560090

    申请日:2021-12-22

    Abstract: The present disclosure provides a device and method of generating a nonlinear waveform signal dissipating low power and operating at a high speed. The device includes: a digital preprocessing unit configured to quantize an effective input signal to generate a linear data signal and a residual signal that is a difference between the effective input signal and the linear data signal; a nonlinear digital-to-analog conversion circuit (DAC) having a nonlinear relationship between an input and an output and configured to convert the linear data signal into a first analog signal; a linear interpolation DAC configured to convert the residual signal into a second analog signal to enable a generation of a converted analog signal by an addition of the second analog signal to the first analog signal; and an output circuit configured to output the converted analog signal as a nonlinear waveform signal.

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