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    PEPTIDE FUSION PROTEIN
    73.
    发明申请
    PEPTIDE FUSION PROTEIN 有权

    公开(公告)号：US20210332152A1

    公开(公告)日：2021-10-28

    申请号：US17264577

    申请日：2019-08-01

    申请人： KAGOSHIMA UNIVERSITY ,  DAICEL CORPORATION

    发明人： Yuji ITO ,  Seiichi UCHIMURA ,  Hiromichi OKURA

    IPC分类号： C07K19/00 ,  C07K14/435 ,  C07K7/08 ,  C07K1/22 ,  C07K17/00

    摘要： An object of the present invention is to provide a method by which a peptide having a specific binding capability that can be used for purification of a target molecule can be produced at a low cost, and specifically relates to a peptide fusion protein including one or more peptides having specific binding capability and a scaffold protein, the peptide being inserted into the amino acid sequence of the scaffold protein directly or via a peptide linker, and/or being linked to the N-terminal and/or C-terminal of the scaffold protein.

    Methods, compositions and systems for production of recombinant spider silk polypeptides
    75.
    发明授权
    Methods, compositions and systems for production of recombinant spider silk polypeptides 有权

    公开(公告)号：US11147250B2

    公开(公告)日：2021-10-19

    申请号：US16104469

    申请日：2018-08-17

    申请人： EGI-LENDERS, LLC

    发明人： David L. Brigham

    IPC分类号： A01K67/033 ,  C07K14/435 ,  C12N15/90 ,  C12N15/85 ,  C07K14/78

    摘要： Disclosed are methods, compositions, and systems for transforming silkworms to produce spider silk and analogs of spider silk. In certain embodiments, the method may include inserting a DNA sequence coding for at least a portion of a spider silk fibroin polypeptide, or an analog of a spider silk fibroin polypeptide, positioned between at least a portion of the 5′ and 3′ ends of a silkworm fibroin gene to generate a fusion gene construct having a sequence that encodes for a polypeptide comprising both spider silk fibroin and silkworm silk fibroin sequences. In certain embodiments, the fused gene is able to replace a native gene present in the silkworm such that the transformed silkworm expresses a polypeptide comprising a spider silk fibroin polypeptide, or an analog thereof, and expresses significantly less of the native silkworm silk.

    SYSTEM AND METHOD OF TRANSFORMING A PROTEIN TO EXHIBIT QUANTUM PROPERTIES AND APPLICATIONS THEREOF
    77.
    发明申请
    SYSTEM AND METHOD OF TRANSFORMING A PROTEIN TO EXHIBIT QUANTUM PROPERTIES AND APPLICATIONS THEREOF 有权

    公开(公告)号：US20210310949A1

    公开(公告)日：2021-10-07

    申请号：US17219108

    申请日：2021-03-31

    申请人： INDIAN INSTITUTE OF SCIENCE EDUCATION AND RESEARCH

    发明人： Kamal Priya SINGH ,  Mehra Singh SIDHU ,  Biswajit PANDA

    IPC分类号： G01N21/64 ,  C09K11/06 ,  C07K14/435

    摘要： Disclosed herein is a novel phenomenon to create a nano-confined, dopant-free, electron spin-dependent fluorescence (SDF) in spider silk by fundamentally transforming its local molecular structure with femtosecond-pulses (206), having fluence below an ablation threshold. Electron-spin dependence of the fluorescent patterns created on the silk sample are confirmed by measuring the fluorescence intensity at different microwave frequencies. The fluorescent intensity exhibits microwave magnetic resonances at 2.88 GHz and 1.44 GHz at room-temperature. The SDF in laser-transformed silk can thereby enable a new-class of tough yet elastic silk-based quantum sensor and hybrid nano-mechanical ultrasensitive cantilevers on a micro-chip. X-ray diffraction (XRD), Raman-spectroscopy, direct atomistic imaging with high-resolution transmission electron microscopy (HR-TEM) and model-building studies are carried out to exhibit the change in the molecular structure and unveil creation of crown-ring like structure in nanocrystals of fluorescent silk with localized electrons possessing mid-gap states.

    NOVEL ANTIMICROBIAL PROTEINS
    78.
    发明申请
    NOVEL ANTIMICROBIAL PROTEINS 有权

    公开(公告)号：US20210300979A1

    公开(公告)日：2021-09-30

    申请号：US17057933

    申请日：2019-05-29

    申请人： LYSANDO AG

    发明人： Manfred BIEBL ,  Martin GRIESSL

    IPC分类号： C07K14/47 ,  C12N9/36 ,  A01N63/50 ,  C07K14/435 ,  A61P31/04

    摘要： The present invention relates to a polypeptide comprising a Gram negative endolysin and a peptide selected from the group consisting of an antimicrobial peptide, an amphipathic peptide, a cationic peptide, a sushi peptide or a defensin, wherein the endolysin in turn is selected from the group consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9 and sequences having at least 80% sequence identity with SEQ ID NO:1, SEQ ID NO:2, SEQ ID NOG, SEQ ID NO:4, SEQ ID NOG, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8 and/or SEQ ID NO:9. The present invention relates also to corresponding nucleic acids, vectors, bacteriophages, host cells, compositions and kits. The present inventions also relates to the use of said polypeptides, nucleic acids, vectors, bacteriophages, host cells, compositions and kits in methods for treatment of the human or animal body by surgery or therapy or in diagnostic methods practiced on the human or animal body. The polypeptides, nucleic acids, vectors, bacteriophages, host cells, compositions and kits according to the invention may also be used as an antimicrobial in, e.g., food or feed, in cosmetics, or as disinfecting agent.

    OPTOGENETIC TOOL FOR RAPID AND REVERSIBLE CLUSTERING OF PROTEINS
    80.
    发明申请
    OPTOGENETIC TOOL FOR RAPID AND REVERSIBLE CLUSTERING OF PROTEINS 有权

    公开(公告)号：US20210292735A1

    公开(公告)日：2021-09-23

    申请号：US17338819

    申请日：2021-06-04

    申请人： The Trustees of Princeton University

    发明人： Cliff Brangwynne ,  Jared Toettcher ,  Yongdae Shin

    IPC分类号： C12N9/88 ,  C07K14/435 ,  C12N13/00 ,  G01N21/00 ,  C12P21/02 ,  C12N15/62 ,  C07K1/32

    摘要： A protein construct including a gene encoding a light-sensitive protein fused to at least one of either a low complexity sequence, an intrinsically disordered protein region (IDR), or a repeating sequence of a linker and another gene encoding a light-sensitive protein. Among the many different possibilities contemplated, the protein construct may also advantageously include cleavage tags. This protein construct may be utilized for a variety of functions, including a method for protein purification, which requires introducing the protein construct into a living cell, and inducing the formation of clusters by irradiating the construct with light. The method may also advantageously include cleaving a target protein from an IDR, and separating the clusters via centrifuge. A kit for practicing in vivo aggregation or liquid-liquid phase separation is also included, the kit including the protein construct and a light source capable of producing a wavelength that the light-sensitive protein will respond to.