公开(公告)号：US20200224186A1

公开(公告)日：2020-07-16

申请号：US16676253

申请日：2019-11-06

申请人： UNIVERSITY OF WASHINGTON ,  UNIVERSITY OF UTAH

发明人： Neil KING ,  Wesley SUNDQUIST ,  Joerg VOTTELER ,  Yang HSIA ,  David BAKER ,  Jacob BALE ,  Marc LAJOIE ,  Gabriel BUTTERFIELD ,  Elizabeth GRAY ,  Daniel STETSON

IPC分类号： C12N9/88 ,  C07K14/00 ,  C07K14/435

摘要： The application discloses multimeric assemblies including multiple oligomeric substructures, where each oligomeric substructure includes multiple proteins that self-interact around at least one axis of rotational symmetry, where each protein includes one or more polypeptide-polypeptide interface (“O interface”); and one or more polypeptide domain that is capable of effecting membrane scission and release of an enveloped multimeric assembly from a cell by recruiting the ESCRT machinery to the site of budding by binding to one or more proteins in the eukaryotic ESCRT complex (“L domain”); and where the multimeric assembly includes one or more subunits comprising one or more polypeptide domain that is capable of interacting with a lipid bilayer (“M domain”), as well as membrane-enveloped versions of the multimeric assemblies.

公开(公告)号：US20210340519A1

公开(公告)日：2021-11-04

申请号：US17336889

申请日：2021-06-02

申请人： UNIVERSITY OF WASHINGTON ,  UNIVERSITY OF UTAH

发明人： Neil KING ,  Wesley SUNDQUIST ,  Joerg VOTTELER ,  Yang HSIA ,  David BAKER ,  Jacob BALE ,  Marc LAJOIE ,  Gabriel BUTTERFIELD ,  Elizabeth GRAY ,  Daniel STETSON

IPC分类号： C12N9/88 ,  C07K14/00 ,  C07K14/435

摘要： The application discloses multimeric assemblies including multiple oligomeric substructures, where each oligomeric substructure includes multiple proteins that self-interact around at least one axis of rotational symmetry, where each protein includes one or more polypeptide-polypeptide interface (“O interface”); and one or more polypeptide domain that is capable of effecting membrane scission and release of an enveloped multimeric assembly from a cell by recruiting the ESCRT machinery to the site of budding by binding to one or more proteins in the eukaryotic ESCRT complex (“L domain”); and where the multimeric assembly includes one or more subunits comprising one or more polypeptide domain that is capable of interacting with a lipid bilayer (“M domain”), as well as membrane-enveloped versions of the multimeric assemblies.

公开(公告)号：US20180030429A1

公开(公告)日：2018-02-01

申请号：US15541201

申请日：2016-02-29

申请人： UNIVERSITY OF WASHINGTON ,  UNIVERSITY OF UTAH

发明人： Neil KING ,  Wesley SUNDQUIST ,  Joerg VOTTELER ,  Yang HSIA ,  David BAKER ,  Jacob BALE ,  Marc LAJOIE ,  Gabriel BUTTERFIELD ,  Elizabeth GRAY ,  Daniel STETSON

IPC分类号： C12N9/88

CPC分类号： C12N9/88 ,  C07K14/00 ,  C07K14/435 ,  C07K2319/03 ,  C07K2319/06 ,  C07K2319/735 ,  C12Y401/02014

摘要： The application discloses multimeric assemblies including multiple oligomeric substructures, where each oligomeric substructure includes multiple proteins that self-interact around at least one axis of rotational symmetry, where each protein includes one or more polypeptide-polypeptide interface (“O interface”); and one or more polypeptide domain that is capable of effecting membrane scission and release of an enveloped multimeric assembly from a cell by recruiting the ESCRT machinery to the site of budding by binding to one or more proteins in the eukaryotic ESCRT complex (“L domain”); and where the multimeric assembly includes one or more subunits comprising one or more polypeptide domain that is capable of interacting with a lipid bilayer (“M domain”), as well as membrane-enveloped versions of the multimeric assemblies.

公开(公告)号：US20210380641A1

公开(公告)日：2021-12-09

申请号：US16762565

申请日：2018-11-09

申请人： UNIVERSITY OF WASHINGTON

发明人： Gabriel BUTTERFIELD ,  Marc Joseph LAJOIE ,  Neil P. KING ,  David BAKER ,  Daniel ELLIS

IPC分类号： C07K14/00 ,  C12N15/87 ,  B82Y40/00

摘要： Synthetic nanostructures, polypeptides that are useful, for example, in making synthetic nanostructures, and methods for using synthetic nanostructures are disclosed herein.