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    PAS kinase assays
    71.
    发明授权
    PAS kinase assays 有权
    PAS激酶测定

    公开(公告)号：US08859214B1

    公开(公告)日：2014-10-14

    申请号：US13180704

    申请日：2011-07-12

    Applicant: Jared Rutter ,  Wojciech Swiatek

    Inventor： Jared Rutter ,  Wojciech Swiatek

    IPC: G01N33/53 ,  G01N31/00

    CPC classification number: G01N33/573 ,  G01N33/6842 ,  G01N2333/91205

    Abstract: The present invention is directed towards methods for measuring and assaying PAS Kinase activity. The methods are useful, for example, for detecting PASK activity in a cell, and for screening for small molecule regulators of PAS kinase activity, as well as characterizing endogenous factors and stimuli that modulate PAS kinase activity, and identifying and optimizing the activity of potential PAS kinase inhibitors.

    Abstract translation: 本发明涉及测量和测定PAS激酶活性的方法。 所述方法例如用于检测细胞中的PASK活性，以及​​筛选PAS激酶活性的小分子调节剂，以及表征调节PAS激酶活性的内源因子和刺激物，以及鉴定和优化潜在的活性 PAS激酶抑制剂。

    METHOD FOR DETECTING BIOMOLECULES
    72.
    发明申请
    METHOD FOR DETECTING BIOMOLECULES 审中-公开
    检测生物分子的方法

    公开(公告)号：US20140248715A1

    公开(公告)日：2014-09-04

    申请号：US14235598

    申请日：2012-06-27

    Applicant: Markus Templin ,  Fridolin Treindl ,  Anette Doettinger ,  Oliver Poetz

    Inventor： Markus Templin ,  Fridolin Treindl ,  Anette Doettinger ,  Oliver Poetz

    IPC: G01N33/543

    CPC classification number: G01N33/54306 ,  G01N33/54313 ,  G01N33/6842

    Abstract: A method of detecting biomolecules present in a complex biological sample includes a) separating the biological sample into fractions according to at least one physical property of the biomolecules; and b) specifically detecting biomolecules present in the fractions using at least one solid-phase-based, immunological detection method including immobilizing the biomolecules from the individual fractions on microsphere populations specific for each fraction and distinguishable from one another.

    Abstract translation: 检测存在于复合生物样品中的生物分子的方法包括：a）根据生物分子的至少一种物理性质将生物样品分离成级分; 和b）使用至少一种基于固相的免疫学检测方法来特异性地检测部分中存在的生物分子，包括将来自各个级分的生物分子固定在对于每个级分特异并且彼此区分的微球群上。

    METHODS FOR MEASURING HDL SUBPOPULATIONS
    73.
    发明申请
    METHODS FOR MEASURING HDL SUBPOPULATIONS 审中-公开
    测定HDL SUBPOPULATIONS的方法

    公开(公告)号：US20140243233A1

    公开(公告)日：2014-08-28

    申请号：US14236501

    申请日：2012-08-02

    Applicant: Scott W. Altmann

    Inventor： Scott W. Altmann

    IPC: G01N33/92

    CPC classification number: G01N33/92 ,  G01N33/487 ,  G01N33/6842 ,  G01N2333/775 ,  G01N2800/044 ,  G01N2800/50 ,  G01N2800/52

    Abstract: This invention provides a capture/detection antibody-based method for measuring the amount of a high density lipoprotein (HDL) subpopulation present in a sample, wherein each particle of the HDL subpopulation being measured is characterized by the presence of a plurality of defined protein epitopes. This invention also provides related analytical and diagnostic methods, as well as kits for performing same.

    Abstract translation: 本发明提供了用于测量样品中存在的高密度脂蛋白（HDL）亚群的量的基于捕获/检测抗体的方法，其中所测量的HDL亚群的每个颗粒的特征在于存在多个限定的蛋白质表位 。 本发明还提供相关的分析和诊断方法，以及用于执行相同的试剂盒。

    Apparatuses, Methods, Computer Program Products, And Kits for Hi-Throughput Glycan Analysis
    75.
    发明申请
    Apparatuses, Methods, Computer Program Products, And Kits for Hi-Throughput Glycan Analysis 审中-公开
    设备，方法，计算机程序产品和用于高吞吐量糖分析的套件

    公开(公告)号：US20140200148A1

    公开(公告)日：2014-07-17

    申请号：US14238183

    申请日：2012-08-10

    Applicant: Peter Slade

    Inventor： Peter Slade

    IPC: G01N27/447 ,  C12Q1/34 ,  G01N33/68

    CPC classification number: G01N33/6842 ,  C12Q1/34 ,  G01N27/44726 ,  G01N27/44782 ,  G01N27/44791 ,  G01N33/6803 ,  G01N2333/924 ,  G01N2400/00 ,  G01N2400/38

    Abstract: An apparatus for glycan analysis is disclosed. The apparatus includes a plurality of loading wells adapted to receive a plurality of samples; a plurality of capillaries arranged in correspondence with the loading wells, each of the capillaries including a first portion including a stacking gel and a second portion including a resolving gel; and a plurality of eluting wells arranged in correspondence with the capillaries and adapted to receive a portion of the samples having traversed the capillaries.

    Abstract translation: 公开了一种用于聚糖分析的装置。 该装置包括适于接收多个样本的多个加载井; 多个毛细管，其布置成与所述装载孔相对应，每个所述毛细管包括包括堆积凝胶的第一部分和包括分解凝胶的第二部分; 以及与毛细管相对应地布置并适于接收穿过毛细管的一部分样品的多个洗脱液。

    Method, system, and computer program product for scoring theoretical peptides
    78.
    发明授权
    Method, system, and computer program product for scoring theoretical peptides 有权
    方法，系统和计算机程序产品，用于评分理论肽

    公开(公告)号：US08712695B2

    公开(公告)日：2014-04-29

    申请号：US11246722

    申请日：2005-10-06

    Applicant: Ignat V. Shilov ,  Sean L. Seymour ,  Ron Bonner ,  Alpesh A. Patel

    Inventor： Ignat V. Shilov ,  Sean L. Seymour ,  Ron Bonner ,  Alpesh A. Patel

    IPC: G01N33/48 ,  G06F19/00 ,  C12N15/87

    CPC classification number: G06F19/00 ,  C12N15/87 ,  G01N33/6842 ,  G01N33/6848 ,  G06F19/22 ,  G06F19/28

    Abstract: The present teachings provide for identification of peptides using small sequence tags to focus computational resources on searching regions of a protein database that are the most likely to yield correct identifications. They allow for the incorporation of modifications and in doing so focuses the search to peptides with a precursor mass match. Additionally, probability or relevance factors can be used to determine peptide hypotheses. Various embodiments are presented that search for peptides when a single precursor is selected or when multiple precursors are simultaneously fragmented.

    Abstract translation: 本教导提供使用小序列标签鉴定肽，以将计算资源集中在最有可能产生正确鉴定的蛋白质数据库的搜索区域上。 它们允许引入修饰，这样做可以将搜索重点放在具有前体大量匹配的肽上。 此外，概率或相关因素可用于确定肽假说。 呈现了当选择单一前体时或当多个前体同时分裂时搜索肽的各种实施方案。

    Immunoaffinity Isolation of Modified Peptides From Complex Mixtures
    79.
    发明申请
    Immunoaffinity Isolation of Modified Peptides From Complex Mixtures 有权
    从复杂混合物中修饰肽的免疫亲和分离

    公开(公告)号：US20140094594A1

    公开(公告)日：2014-04-03

    申请号：US13868387

    申请日：2013-04-23

    Applicant: Cell Signaling Technology, Inc.

    Inventor： John Rush ,  Hui Zhang ,  Xiangming Zha ,  Michael J Comb ,  Yi Tan

    IPC: C07K16/18

    CPC classification number: C07K16/18 ,  B01D15/3809 ,  B01D15/3828 ,  C07K1/22 ,  C07K16/44 ,  G01N30/7233 ,  G01N33/6842 ,  G01N33/6848 ,  G01N33/6854 ,  G01N2030/8831 ,  G01N2440/14

    Abstract: The invention provides methods for isolating a modified peptide from a complex mixture of peptides, the method comprising the steps of: (a) obtaining a proteinaceous preparation from an organism, wherein the preparation comprises modified peptides from two or more different proteins; (b) contacting the preparation with at least one immobilized modification-specific antibody; and (c) isolating at least one modified peptide specifically bound by the immobilized modification-specific antibody in step (b). The method may further comprise the step of (d) characterizing the modified peptide isolated in step (c) by mass spectrometry (MS), tandem mass spectrometry (MS-MS), and/or MS3 analysis, or the step of (e) utilizing a search program to substantially match the spectra obtained for the modified peptide during the characterization of step (d) with the spectra for a known peptide sequence, thereby identifying the parent protein(s) of the modified peptide. Also provided are an immunoaffinity isolation device comprising a modification-specific antibody, and antibodies against novel UFD1 and PTN6 phosphorylation sites.

    Abstract translation: 本发明提供了从复杂的肽混合物中分离修饰的肽的方法，所述方法包括以下步骤：（a）从生物体获得蛋白质制剂，其中所述制剂包含来自两种或更多种不同蛋白质的修饰肽; （b）使制剂与至少一种固定的修饰特异性抗体接触; 和（c）在步骤（b）中分离至少一种由固定的修饰特异性抗体特异性结合的修饰的肽。 该方法还可以包括以下步骤：（d）通过质谱（MS），串联质谱（MS-MS）和/或MS3分析表征步骤（c）中分离的修饰肽，或（e） 利用搜索程序将在步骤（d）表征期间获得的修饰肽的光谱与已知肽序列的光谱基本上匹配，从而鉴定修饰肽的亲本蛋白。 还提供了包含修饰特异性抗体和针对新型UFD1和PTN6磷酸化位点的抗体的免疫亲和分离装置。

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