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184 条记录 (耗时 0.161 秒)

    PROCESS FOR PRODUCTION OF RECOMBINANT PROTEINS AS A SOLUBLE FORM
    77.
    发明申请
    PROCESS FOR PRODUCTION OF RECOMBINANT PROTEINS AS A SOLUBLE FORM 有权
    生产重组蛋白作为可溶形式的方法

    公开(公告)号：US20150087047A1

    公开(公告)日：2015-03-26

    申请号：US14476624

    申请日：2014-09-03

    申请人： JNC CORPORATION

    发明人： Satoshi INOUYE ,  Yuiko SAHARA

    IPC分类号： C07K14/31 ,  C12N9/02

    CPC分类号： C07K14/31 ,  C07K2319/61 ,  C07K2319/705 ,  C12N9/0069 ,  C12N15/62 ,  C12Y113/12005 ,  C12Y113/12007 ,  C12Y113/12013

    摘要： A target protein is prepared as soluble protein using a recombinant protein expression system. An expression vector is used that includes (1) an expression-inducible promoter sequence; (2) a first coding sequence including a polynucleotide coding for a polypeptide that is represented by the formula (Z)n; and (3) a second coding sequence that includes a polynucleotide that codes for a target protein. A method of producing the target protein is also used that includes expressing protein using this expression vector.

    摘要翻译： 使用重组蛋白表达系统制备靶蛋白作为可溶性蛋白质。 使用表达载体，其包括（1）表达诱导型启动子序列; （2）包含编码由式（Z）n表示的多肽的多核苷酸的第一编码序列; 和（3）包含编码靶蛋白的多核苷酸的第二编码序列。 还使用包含使用该表达载体表达蛋白质的靶蛋白质的制造方法。

    Method for binding a protein consisting of protein A or consisting of at least one domain of the A to E domains of the protein A to the substrate
    79.
    发明授权
    Method for binding a protein consisting of protein A or consisting of at least one domain of the A to E domains of the protein A to the substrate 有权
    由蛋白A组成或由蛋白A的A至E结构域的至少一个结构域组成的蛋白质与底物结合的方法

    公开(公告)号：US08859294B2

    公开(公告)日：2014-10-14

    申请号：US13074973

    申请日：2011-03-29

    申请人： Jin Muraoka ,  Takachika Azuma ,  Akikazu Murakami

    发明人： Jin Muraoka ,  Takachika Azuma ,  Akikazu Murakami

    IPC分类号： G01N33/68 ,  C07K17/14 ,  C07K14/31 ,  C07K17/02 ,  C07K7/06

    CPC分类号： C07K17/14 ,  C07K7/06 ,  C07K2319/705

    摘要： The object of the present invention is to provide a method for immobilizing the SpA protein on the surface of a substrate with high density without causing dimerization.The following method solves the object. That is, the method for binding a protein to a surface of a substrate, comprising steps (A) to (B): step (A) of preparing said protein to the surface, step (B) of supplying said protein to the surface, wherein said protein consists of a Protein A or at least one domain of A to E of said Protein A, and said protein comprises C-terminal modified amino acid sequence represented by SEQ ID:1(SFNRSEC).

    摘要翻译： 本发明的目的是提供一种用于将SpA蛋白固定在基底表面上，而不会引起二聚化的方法，该方法具有高密度。 以下方法可以解决对象。 也就是说，将蛋白质与底物表面结合的方法，包括步骤（A）至（B）：将表面蛋白质制备的步骤（A），向表面供给所述蛋白质的步骤（B） 其中所述蛋白质由蛋白A或所述蛋白A的A至E的至少一个结构域组成，所述蛋白质包含由SEQ ID：1（SFNRSEC）表示的C末端修饰的氨基酸序列。