公开(公告)号：US10234392B2

公开(公告)日：2019-03-19

申请号：US15416976

申请日：2017-01-26

Applicant: ACEA Biosciences, Inc.

Inventor： Nan Li ,  Ye Chen ,  Hyunsun Chung ,  Zengqiang Li ,  Xiaobo Wang

IPC: G01N21/64 ,  G01N33/569 ,  G01N15/14 ,  G01N15/10

Abstract: An optical engine for use in a bench top flow cytometer, the optical engine comprising a set of lasers; a different set of beam shaping optics for each laser, wherein each set comprises two lenses to adjustably focus light horizontally along an x-axis to a same horizontal position and vertically along a y-axis to a different vertical position along a same plane; collection optics for collecting fluorescence from the flow cell; filtration optics that filter the collected fluorescence from the flow cell into different detection channels according to wavelength ranges; and a detector for each detection channel that converts the filtered fluorescence to electrical signals, wherein electrical signals are processed so that the fluorescence from each laser at the different vertical positions is distinguished at the same detector.

公开(公告)号：US10012636B2

公开(公告)日：2018-07-03

申请号：US15651882

申请日：2017-07-17

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaobo Wang ,  Yama A. Abassi ,  Biao Xi ,  Wen Fu Zhang ,  Xiao Xu

IPC: C40B30/06 ,  G01N7/00 ,  C12Q1/00 ,  G01N33/483

CPC classification number: G01N33/4836

Abstract: A system for monitoring impedance of excitable cells in vitro, which includes a device for monitoring cell-substrate impedance at 20 millisecond resolution, which includes a nonconductive substrate with one or more electrode arrays fabricated in one or more wells, wherein cell attachment on the substrate can result in a detectable change in impedance between electrodes within each electrode array; an impedance analyzer capable of impedance measurement at 20 millisecond time resolution; electronic circuitry that can engage said device and selectively connect said two or more electrode arrays of said device to said impedance analyzer; and a software program that controls said electronic circuitry and records and analyzes data obtained from said impedance analyzer.

公开(公告)号：US09920074B2

公开(公告)日：2018-03-20

申请号：US15271124

申请日：2016-09-20

Applicant: ACEA BIOSCIENCES INC.

Inventor： Xiao Xu ,  Xiaobo Wang ,  Long Mao ,  Li Zhao ,  Biao Xi

IPC: C07D519/00 ,  C07D487/04 ,  C07D239/49 ,  C07D239/52 ,  C07D403/12 ,  C07D401/12 ,  C07D473/18 ,  C07D417/12 ,  C07D239/47 ,  C07D239/48

CPC classification number: C07D519/00 ,  C07D239/47 ,  C07D239/48 ,  C07D239/49 ,  C07D239/52 ,  C07D401/12 ,  C07D403/12 ,  C07D417/12 ,  C07D473/18 ,  C07D487/04

Abstract: The present invention relates to pharmaceutical compounds, compositions and methods, especially as they are related to compositions and methods for the treatment and/or prevention of a proliferation disorder, a cancer, a tumor, an inflammatory disease, an autoimmune disease, psoriasis, dry eye or an immunologically related disease, and in some embodiments diseases or disorders related to the dysregulation of kinase such as, but not limited to, EGFR (including HER), Alk, PDGFR, BLK, BMX/ETK, FLT3(D835Y), ITK, TEC, TXK, BTK, or JAK, and the respective pathways.

公开(公告)号：US09709548B2

公开(公告)日：2017-07-18

申请号：US12435569

申请日：2009-05-05

Applicant: Xiaobo Wang ,  Yama A. Abassi ,  Biao Xi ,  Wen Fu Zhang ,  Xiao Xu

Inventor： Xiaobo Wang ,  Yama A. Abassi ,  Biao Xi ,  Wen Fu Zhang ,  Xiao Xu

IPC: C40B30/06 ,  G01N7/00 ,  C12Q1/00 ,  G01N33/483

CPC classification number: G01N33/4836

Abstract: Systems and methods for improved monitoring of excitation-contraction coupling and excitable cells are provided, which provide millisecond time resolution. The system is capable of continuously monitoring excitation-contraction coupling in a relatively high-throughput manner. The system includes a device for monitoring cell-substrate impedance, an impedance analyzer capable of impedance measurements at millisecond time resolution, electronic circuitry that can engage the device and selectively connect two or more electrode arrays of the device to the impedance analyzer and a software program that controls the electronic circuitry and records and analyzes data obtained from the impedance analyzer.

公开(公告)号：US09625472B2

公开(公告)日：2017-04-18

申请号：US13244004

申请日：2011-09-23

Applicant: Xiao Xu ,  Yama Abassi ,  Xiaobo Wang ,  Jiangbo Gan

Inventor： Xiao Xu ,  Yama Abassi ,  Xiaobo Wang ,  Jiangbo Gan

IPC: C12M1/34 ,  G01N33/68 ,  C12M1/12 ,  G01N33/50 ,  G01N33/483

CPC classification number: G01N33/6854 ,  C12M25/08 ,  C12M41/36 ,  G01N33/4836 ,  G01N33/5005 ,  G01N2500/10

Abstract: The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays.

公开(公告)号：US08685988B2

公开(公告)日：2014-04-01

申请号：US13917514

申请日：2013-06-13

Applicant: ACEA Biosciences Inc.

Inventor： Xiao Xu ,  Xiaobo Wang ,  Long Mao ,  Li Zhao ,  Biao Xi

IPC: A01N43/90 ,  A61K31/519 ,  C07D487/00

CPC classification number: A61K31/519 ,  A61K45/06 ,  C07D487/04 ,  A61K2300/00

Abstract: The present invention relates to certain pyrrolopyrimidine derivatives, pharmaceutical compositions containing them, and methods of using them, including methods for the treatment of tumors and related diseases related to the dysregulation of kinase (such as EGFR (including HER), Alk, PDGFR, but not limited to) pathways.

Abstract translation: 本发明涉及某些吡咯并嘧啶衍生物，含有它们的药物组合物及其使用方法，包括治疗肿瘤的方法和与激酶失调相关的相关疾病（如EGFR（包括HER），Alk，PDGFR），但是 途径。

公开(公告)号：US08334316B2

公开(公告)日：2012-12-18

申请号：US12368206

申请日：2009-02-09

Applicant: Xiao Xu ,  Haoyun An ,  Xiaobo Wang

Inventor： Xiao Xu ,  Haoyun An ,  Xiaobo Wang

IPC: A61K31/343 ,  A61K31/381 ,  A61K31/40 ,  A61K31/4025 ,  A61K31/404 ,  A61K31/41 ,  A61K31/415 ,  A61K31/4164 ,  A61K31/4184 ,  A61K31/4192 ,  A61K31/42 ,  A61K31/425 ,  A61K31/428 ,  A61K31/433 ,  A61K31/44 ,  A61K31/4427 ,  A61K31/47 ,  A61K31/495 ,  A61K31/497 ,  A61K31/50 ,  A61K31/505 ,  A61K31/53 ,  C07D279/08 ,  C07D277/02 ,  C07D277/62 ,  C07D275/02 ,  C07D285/02 ,  C07D271/02 ,  C07D263/02 ,  C07D261/02 ,  C07D251/02 ,  C07D249/02 ,  C07D241/02 ,  C07D241/46 ,  C07D239/02 ,  C07D237/02 ,  C07D237/28 ,  C07D237/30 ,  C07D235/04 ,  C07D233/54 ,  C07D231/02 ,  C07D219/00 ,  C07D217/00 ,  C07D215/00 ,  C07D213/02 ,  C07D209/04 ,  C07D207/30

CPC classification number: C07C323/07 ,  C07C321/20 ,  C07C323/16 ,  C07C323/20

Abstract: The present invention provides substituted di-, tri-, tetra- and penta-sulfide compounds and compositions, and methods of using the same for the treatment and/or prevention of a cell proliferative disorder. The present invention also provides methods for preparing trisulfide compounds and compositions.

Abstract translation: 本发明提供取代的二，三，四和五硫化物化合物和组合物，及其用于治疗和/或预防细胞增殖性疾病的方法。 本发明还提供了制备三硫化物和组合物的方法。

公开(公告)号：US20120225424A1

公开(公告)日：2012-09-06

申请号：US13474355

申请日：2012-05-17

Applicant: Yama A. Abassi ,  Xiaobo Wang ,  Josephine Atienza ,  Xiao Xu ,  Junquan Xu

Inventor： Yama A. Abassi ,  Xiaobo Wang ,  Josephine Atienza ,  Xiao Xu ,  Junquan Xu

IPC: G01N27/02 ,  G01N21/00 ,  C12M1/42 ,  G01N21/64

CPC classification number: C12N15/87 ,  C12M35/02 ,  G01N15/12 ,  G01N33/48728

Abstract: The present invention includes devices and methods for transfecting a cell or cell population and dynamic monitoring of cellular events. A variety of microelectronic devices are provide that incorporate functions such as electroporation, modulation of a transmembrane potential and dynamic monitoring of cellular functions and mechanisms.

Abstract translation: 本发明包括用于转染细胞或细胞群的装置和方法以及细胞事件的动态监测。 提供了多种微电子器件，其包括诸如电穿孔，跨膜电位的调节和细胞功能和机制的动态监测的功能。

公开(公告)号：US08026080B2

公开(公告)日：2011-09-27

申请号：US11725040

申请日：2007-03-15

Applicant: Xiaobo Wang ,  Yama A. Abassi ,  Xiao Xu ,  Jiangbo Gan

Inventor： Xiaobo Wang ,  Yama A. Abassi ,  Xiao Xu ,  Jiangbo Gan

IPC: C12Q1/20 ,  C12Q1/08 ,  C12M1/34

CPC classification number: G01N33/6854 ,  C12M25/08 ,  C12M41/36 ,  G01N33/4836 ,  G01N33/5005 ,  G01N2500/10

Abstract: The present invention includes devices, systems, and methods for assaying cells using cell-substrate impedance monitoring. In one aspect, the invention provides cell-substrate impedance monitoring devices that comprise electrode arrays on a nonconducting substrate, in which each of the arrays has an approximately uniform electrode resistance across the entire array. In another aspect, the invention provides cell-substrate monitoring systems comprising one or more cell-substrate monitoring devices comprising multiple wells each having an electrode array, an impedance analyzer, a device station that connects arrays of individual wells to the impedance analyzer, and software for controlling the device station and impedance analyzer. In another aspect, the invention provides cellular assays that use impedance monitoring to detect changes in cell behavior or state. In some preferred aspects, the assays are designed to investigate the affects of compounds on cells, such as cytotoxicity assays. In other preferred aspects, the assays are designed to investigate the compounds that effect IgE-mediated responses of cells to antigens.

Abstract translation: 本发明包括使用细胞 - 基底阻抗监测来测定细胞的装置，系统和方法。 在一个方面，本发明提供了包括非导电衬底上的电极阵列的电池衬底阻抗监测装置，其中每个阵列在整个阵列上具有大致均匀的电极电阻。 在另一方面，本发明提供了一种电池衬底监测系统，其包括一个或多个电池衬底监测装置，其中每个电池衬底监测装置包括各自具有电极阵列的多个阱，阻抗分析器，将各个阱的阵列连接到阻抗分析仪的装置站以及软件 用于控制设备站和阻抗分析仪。 在另一方面，本发明提供了使用阻抗监测来检测细胞行为或状态的变化的细胞测定。 在一些优选的方面，设计测定法以研究化合物对细胞的影响，例如细胞毒性测定。 在其它优选方面，设计这些测定法来研究影响细胞对抗原的IgE介导的反应的化合物。

公开(公告)号：US07851657B2

公开(公告)日：2010-12-14

申请号：US12413385

申请日：2009-03-27

Applicant: Haoyun An ,  Xiaopeng Mo

Inventor： Haoyun An ,  Xiaopeng Mo

IPC: C07C321/00

CPC classification number: C07C319/24 ,  C07C319/28 ,  C07C323/07

Abstract: The invention provides a synthetic and manufacturing process for the preparation of the anticancer drug, fluorapacin, bis(4-fluorobenzyl)trisulfide, and related trisulfide derivatives on large scale. Also provided are processes for the purification and isolation of fluorapacin having high purity and improved stability.

Abstract translation: 本发明提供了大规模制备抗癌药物，氟卡宾，双（4-氟苄基）三硫化物和相关三硫​​化物衍生物的合成和制备方法。 还提供了用于纯化和分离具有高纯度和改善的稳定性的氟沙平的方法。