公开(公告)号：US07670783B2

公开(公告)日：2010-03-02

申请号：US11760668

申请日：2007-06-08

Applicant: Paul A Cox ,  Sandra Banack ,  Susan Murch

Inventor： Paul A Cox ,  Sandra Banack ,  Susan Murch

IPC: G01N33/48 ,  G01N33/50

CPC classification number: G01N33/6896 ,  G01N33/6812 ,  G01N33/94 ,  G01N2410/00 ,  G01N2550/00 ,  G01N2800/28 ,  Y02A50/57

Abstract: Methods for screening for neurological disorders are disclosed. Specifically, methods are disclosed for screening for neurological disorders in a subject by analyzing a tissue sample obtained from the subject for the presence of elevated levels of neurotoxic amino acids or neurotoxic derivatives thereof associated with neurological disorders. In particular, methods are disclosed for diagnosing a neurological disorder in a subject, or predicting the likelihood of developing a neurological disorder in a subject, by determining the levels of β-N-methylamino-L-alanine (BMAA) in a tissue sample obtained from the subject. Methods for screening for environmental factors associated with neurological disorders are disclosed. Methods for inhibiting, treating or preventing neurological disorders are disclosed.

Abstract translation: 公开了筛选神经障碍的方法。 具体地，公开了通过分析从受试者获得的组织样品中存在与神经系统疾病相关的神经毒性氨基酸或神经毒性衍生物的升高水平来筛选受试者神经障碍的方法。 具体地，通过确定组织样品中的B-甲基氨基-L-丙氨酸（BMAA）的水平，公开了用于诊断受试者神经障碍或预测受试者发展神经障碍的可能性的方法 从主题获得。 公开了与神经障碍相关的环境因素的筛选方法。 公开了抑制，治疗或预防神经障碍的方法。

公开(公告)号：US07655471B2

公开(公告)日：2010-02-02

申请号：US10347169

申请日：2003-01-17

Applicant: Inger Byrjalsen ,  Peter M. Larsen ,  Stephen J. Fey

Inventor： Inger Byrjalsen ,  Peter M. Larsen ,  Stephen J. Fey

IPC: G01N33/50 ,  G01N33/53

CPC classification number: G01N33/6803 ,  G01N33/574 ,  G01N2550/00

Abstract: Endometrium secreted polypeptides are assayed in body fluids to determine the presence of polypeptides of specified pI and MW values that have been found to be regulated in body fluids according to the status of the endometrium, or the presence of hyperplasia or adenocarcinoma.

Abstract translation: 在体液中测定子宫内膜分泌的多肽，以确定根据子宫内膜的状态，或增生或腺癌的存在，发现已被发现在体液中调节的特定的pI和MW值的多肽的存在。

公开(公告)号：US20090326830A1

公开(公告)日：2009-12-31

申请号：US12431221

申请日：2009-04-28

Applicant: Lindsey Foreman ,  Ian Evett ,  Susan Pope ,  John Buckleton ,  James Curran ,  Christopher Triggs

Inventor： Lindsey Foreman ,  Ian Evett ,  Susan Pope ,  John Buckleton ,  James Curran ,  Christopher Triggs

IPC: G06F19/00

CPC classification number: G16B20/00 ,  G01N2550/00

Abstract: Methods for establishing the genotype of a DNA sample, and methods for investigating the potential sources of a DNA sample arising from a plurality of source, are provided, the methods being based on a method including: analysing the sample to produce a data profile for the sample for a locus; proposing a suggested genotype; generating a first stage profile for the locus for the suggested genotype; adjusting the first stage profile to account for one or more factors to give a simulation profile; and comparing the data profile and the simulation profile to provide an indication of the likelihood of the data profile given the suggested genotype. The methods in effect make adjustments to take the first stage profile, potentially through one or more intervening profiles, to the simulation profile, the simulation profile being an anticipation of the data profile which would be expected to occur for that suggested genotype in practice. The methods potentially include adjustments for one or more of preferential amplification and/or stutter and/or allele drop out and/or allele drop in and/or stochastic components and/or noise and/or preferential degradation and/or the relative contributions from the sources.

Abstract translation: 提供了建立DNA样品的基因型的方法以及用于研究由多个源产生的DNA样品的潜在来源的方法，所述方法基于一种方法，包括：分析样品以产生用于 一个地点的样本 提出建议的基因型; 为所建议的基因型产生基因座的第一阶段谱; 调整第一阶段轮廓以考虑一个或多个因素以给出模拟轮廓; 并且比较数据简档和模拟简档，以提供给定建议的基因型的数据简档的可能性的指示。 这些方法实际上可以通过一个或多个介入简档将模拟简档作为第一阶段简档进行调整，该模拟简档是预期将在实践中对该建议的基因型预期发生的数据简档。 这些方法可能包括对优先扩增和/或口吃和/或等位基因脱落和/或等位基因落入和/或随机组分中和/或噪声和/或优先降解和/或来自 来源。

公开(公告)号：US07598047B2

公开(公告)日：2009-10-06

申请号：US10713861

申请日：2003-11-14

Applicant: Jonathan Minden ,  Alan Waggoner ,  Susan Janet Fowler

Inventor： Jonathan Minden ,  Alan Waggoner ,  Susan Janet Fowler

IPC: G01N33/53

CPC classification number: C07K1/13 ,  G01N21/6428 ,  G01N27/44726 ,  G01N33/582 ,  G01N33/6839 ,  G01N2021/6421 ,  G01N2021/6441 ,  G01N2550/00 ,  G01N2800/52 ,  Y10S435/81 ,  Y10S436/80 ,  Y10T436/143333 ,  Y10T436/173845 ,  Y10T436/203332

Abstract: A process and a kit are provided for detecting differences in two or more samples of protein, including proteins bearing post-translational modifications and peptides. Proteins are prepared, for example, from each of a different group of cell samples or body fluid samples to be compared. Each protein extract is labeled with a different one of a luminescent dye from a matched set of dyes. The matched dyes have generally the same ionic and pH characteristics but emit light at different wavelengths to exhibit a different color upon luminescence detection. The labeled protein extracts are mixed together and separated together by electrophoresis or a chromatographic method. The separation is observed to detect proteins unique to one sample or present in a greater ratio in one sample than in the other. Those unique or excess proteins will fluoresce the color of one of the dyes used. Proteins common to each sample migrate together and fluoresce the same.

公开(公告)号：US07462489B2

公开(公告)日：2008-12-09

申请号：US11935048

申请日：2007-11-05

Applicant: Klaus F. Ley ,  David M. Smalley

Inventor： Klaus F. Ley ,  David M. Smalley

IPC: A01N1/02 ,  A01N31/00 ,  A01N33/00

CPC classification number: G01N33/6803 ,  G01N33/6848 ,  G01N2550/00 ,  Y10T436/106664

Abstract: The present invention provides methods for identifying candidate biomarkers in the plasma microparticle proteome. It also provides methods for isolating, identifying, and analyzing microparticles derived from plasma, particularly platelet-poor plasma.

Abstract translation: 本发明提供用于鉴定血浆微粒蛋白质组中候选生物标志物的方法。 它还提供了分离，鉴定和分析衍生自血浆，特别是血小板血浆的微粒的方法。

公开(公告)号：US07432066B2

公开(公告)日：2008-10-07

申请号：US11585354

申请日：2006-10-23

Applicant: Marsha A. Moses ,  Li Yan

Inventor： Marsha A. Moses ,  Li Yan

IPC: G01N33/53

CPC classification number: G01N33/573 ,  C12Q1/37 ,  G01N33/574 ,  G01N33/57434 ,  G01N33/57488 ,  G01N2333/96486 ,  G01N2333/96494 ,  G01N2333/99 ,  G01N2550/00

Abstract: Methods and kits for diagnosing the presence of and prognosing the appearance of tissue remodelling-associated conditions, involving the presence of enzyme complexes in a biological sample, are disclosed. In particular, the method pertains to diagnosing the presence of or prognosing appearance of metastatic cancer by the identification of high molecular weight enzyme complexes comprising MMPs.

Abstract translation: 公开了用于诊断存在并预测组织重塑相关病症的外观的方法和试剂盒，其涉及生物样品中酶复合物的存在。 特别地，该方法涉及通过鉴定包含MMP的高分子量酶复合物来诊断转移性癌症的存在或预后。

公开(公告)号：US20080081343A1

公开(公告)日：2008-04-03

申请号：US11649744

申请日：2007-01-04

Applicant: Paul Haynes ,  Jing Wei ,  John Yates ,  Nancy Andon ,  Michael P. Washburn ,  Cosmin Deciu ,  Ryan U. Ulaszek

Inventor： Paul Haynes ,  Jing Wei ,  John Yates ,  Nancy Andon ,  Michael P. Washburn ,  Cosmin Deciu ,  Ryan U. Ulaszek

IPC: G01N33/53

CPC classification number: G01N33/6803 ,  C07K1/13 ,  C07K14/395 ,  G01N33/6815 ,  G01N33/6842 ,  G01N33/6848 ,  G01N2458/15 ,  G01N2550/00

Abstract: The disclosed subject matter relates to a method of simultaneously identifying and determining the levels of expression of cysteine-containing proteins in normal and perturbed cells, a method for determining peptide expression levels between a first biological sample and a second biological sample, and a method for quantitative proteomic analysis of two or more peptide populations, and compounds and reagents related thereto.

Abstract translation: 所公开的主题涉及同时鉴定和确定正常和扰动细胞中含半胱氨酸的蛋白质的表达水平的方法，用于测定第一生物样品和第二生物样品之间的肽表达水平的方法，以及用于 两个或多个肽群体的定量蛋白质组学分析，以及与其相关的化合物和试剂。

公开(公告)号：US20070293437A1

公开(公告)日：2007-12-20

申请号：US11665539

申请日：2005-10-21

Applicant: Andras Guttman ,  Laszlo Takacs

Inventor： Andras Guttman ,  Laszlo Takacs

IPC: A61K38/00 ,  G01N33/00 ,  G01N33/53

CPC classification number: G01N33/6803 ,  G01N2550/00

Abstract: The present invention relates to an efficient mAb panel-based expression profiling technology platform suitable for global and accurate measurement of proteins, peptides and metabolites in complex mixtures. The platform is comprised of new and well established technologies that are coupled in a unique fashion to provide a novel platform technology for (i) the discovery of differentially displayed elements of complex protein, peptide and metabolite mixtures and (ii) the development of robust mAb based assays that detect the differentially expressed elements.

Abstract translation: 本发明涉及一种有效的基于mAb面板的表达谱分析技术平台，适用于全面准确地测量复杂混合物中的蛋白质，肽和代谢物。 该平台由新的和成熟的技术组成，以独特的方式相结合，为（i）发现差异显示的复杂蛋白质，肽和代谢物混合物的元素提供了一种新的平台技术，以及（ii）开发强大的mAb 检测差异表达的元件。

公开(公告)号：US06969757B2

公开(公告)日：2005-11-29

申请号：US10057789

申请日：2002-01-25

Applicant: Paul Haynes ,  Jing Wei ,  John Yates ,  Nancy Andon

Inventor： Paul Haynes ,  Jing Wei ,  John Yates ,  Nancy Andon

IPC: G01N33/68 ,  C07C235/00 ,  C07K7/06 ,  C07K7/08

CPC classification number: G01N33/6803 ,  G01N2458/15 ,  G01N2550/00

Abstract: The present invention relates to a method of simultaneously identifying and determining the levels of expression of cysteine-containing proteins in normal and perturbed cells, a method for proteomic analysis, a process for preparing fusion proteins, and compounds of Formula II and III: (II) Acyl-NH—X-[Epitope Tag Site]A-Y-[Protease Cleavage Site]-Z-Link (III) Acyl-NH—X-alk-O-Ph-CH2—Z-Link and reagents related thereto.

Abstract translation: 本发明涉及同时鉴定和确定正常和扰动细胞中含半胱氨酸的蛋白质的表达水平，蛋白质组学分析方法，制备融合蛋白的方法和式II和III化合物的方法：（II ）酰基-NH-X- [表位标签位点] A-A- [蛋白酶切割位点] -Z-Link（III）酰基-NH-X-alk-O-Ph-CH _{2 -Z-Link和与之相关的试剂。}

公开(公告)号：US20040022794A1

公开(公告)日：2004-02-05

申请号：US10264309

申请日：2002-10-03

Inventor： L. Kathryn Durham ,  David L. Friedman ,  Herath Mudiyanselage Athula Chandrasiri Herath ,  Lida H. Kimmel ,  Rajesh Bhikhu Parekh ,  David M. Potter ,  Christian Rohlff ,  B. Michael Silber ,  Peter Jeffrey Snyder ,  Holly Daria Soares ,  Thomas R. Stiger ,  P. Trey Sunderland ,  Robert Reid Townsend ,  W. Frost White ,  Stephen A. Williams

IPC: A61K048/00 ,  C12Q001/68 ,  G01N033/53 ,  G01N033/542 ,  A61K039/00 ,  C07K016/40

CPC classification number: G01N33/6896 ,  G01N2333/4709 ,  G01N2550/00 ,  G01N2800/2821 ,  G01N2800/52

Abstract: The present invention provides methods and compositions for screening, diagnosis and prognosis of Alzheimer's disease, for monitoring the effectiveness of Alzheimer's disease treatment, and for drug development. Alzheimer's Disease-Associated Features (AFs), detectable by two-dimensional electrophoresis of cerebrospinal fluid, serum or plasma are described. The invention further provides Alzheimer's Disease-Associated Protein Isoforms (APIs) detectable in cerebrospinal fluid, serum or plasma, preparations comprising isolated APIs, antibodies immunospecific for APIs, pharmaceutical compositions, diagnostic and therapeutic methods, and kits comprising or based on the same.

Abstract translation: 本发明提供了用于阿尔茨海默病筛选，诊断和预后的方法和组合物，用于监测阿尔茨海默病治疗的有效性和用于药物开发。 描述了通过脑脊液，血清或血浆的二维电泳可检测的阿尔茨海默氏病相关特征（AF）。 本发明还提供在脑脊液，血清或血浆中可检测的阿尔茨海默氏病相关蛋白质异构体（API），包含分离的API的制剂，针对API免疫特异性的抗体，药物组合物，诊断和治疗方法，以及包含或基于其的试剂盒。