公开(公告)号：US07166436B2

公开(公告)日：2007-01-23

申请号：US10212628

申请日：2002-08-01

Applicant: Paul Haynes ,  Jing Wei ,  John Yates ,  Nancy Andon

Inventor： Paul Haynes ,  Jing Wei ,  John Yates ,  Nancy Andon

IPC: G01N33/53 ,  C12P13/12 ,  C07K17/00 ,  C07K5/00 ,  C07K16/00 ,  C07K7/00

CPC classification number: G01N33/6803 ,  C07K1/13 ,  C07K14/395 ,  G01N33/6815 ,  G01N33/6842 ,  G01N33/6848 ,  G01N2458/15 ,  G01N2550/00

Abstract: The present invention relates to a method of simultaneously identifying and determining the levels of expression of cysteine-containing proteins in normal and perturbed cells, a method for proteomic analysis, a process for preparing fusion proteins, and compounds and reagents related thereto.

Abstract translation: 本发明涉及同时鉴定和确定正常和扰动细胞中含半胱氨酸的蛋白质的表达水平，蛋白质组分析方法，制备融合蛋白的方法以及与其相关的化合物和试剂的方法。

公开(公告)号：US20080081343A1

公开(公告)日：2008-04-03

申请号：US11649744

申请日：2007-01-04

Applicant: Paul Haynes ,  Jing Wei ,  John Yates ,  Nancy Andon ,  Michael P. Washburn ,  Cosmin Deciu ,  Ryan U. Ulaszek

Inventor： Paul Haynes ,  Jing Wei ,  John Yates ,  Nancy Andon ,  Michael P. Washburn ,  Cosmin Deciu ,  Ryan U. Ulaszek

IPC: G01N33/53

CPC classification number: G01N33/6803 ,  C07K1/13 ,  C07K14/395 ,  G01N33/6815 ,  G01N33/6842 ,  G01N33/6848 ,  G01N2458/15 ,  G01N2550/00

Abstract: The disclosed subject matter relates to a method of simultaneously identifying and determining the levels of expression of cysteine-containing proteins in normal and perturbed cells, a method for determining peptide expression levels between a first biological sample and a second biological sample, and a method for quantitative proteomic analysis of two or more peptide populations, and compounds and reagents related thereto.

Abstract translation: 所公开的主题涉及同时鉴定和确定正常和扰动细胞中含半胱氨酸的蛋白质的表达水平的方法，用于测定第一生物样品和第二生物样品之间的肽表达水平的方法，以及用于 两个或多个肽群体的定量蛋白质组学分析，以及与其相关的化合物和试剂。

公开(公告)号：US06969757B2

公开(公告)日：2005-11-29

申请号：US10057789

申请日：2002-01-25

Applicant: Paul Haynes ,  Jing Wei ,  John Yates ,  Nancy Andon

Inventor： Paul Haynes ,  Jing Wei ,  John Yates ,  Nancy Andon

IPC: G01N33/68 ,  C07C235/00 ,  C07K7/06 ,  C07K7/08

CPC classification number: G01N33/6803 ,  G01N2458/15 ,  G01N2550/00

Abstract: The present invention relates to a method of simultaneously identifying and determining the levels of expression of cysteine-containing proteins in normal and perturbed cells, a method for proteomic analysis, a process for preparing fusion proteins, and compounds of Formula II and III: (II) Acyl-NH—X-[Epitope Tag Site]A-Y-[Protease Cleavage Site]-Z-Link (III) Acyl-NH—X-alk-O-Ph-CH2—Z-Link and reagents related thereto.

Abstract translation: 本发明涉及同时鉴定和确定正常和扰动细胞中含半胱氨酸的蛋白质的表达水平，蛋白质组学分析方法，制备融合蛋白的方法和式II和III化合物的方法：（II ）酰基-NH-X- [表位标签位点] A-A- [蛋白酶切割位点] -Z-Link（III）酰基-NH-X-alk-O-Ph-CH _{2 -Z-Link和与之相关的试剂。}

公开(公告)号：US20050064513A1

公开(公告)日：2005-03-24

申请号：US10901536

申请日：2004-07-29

Applicant: Paul Haynes ,  Nancy Andon

Inventor： Paul Haynes ,  Nancy Andon

IPC: G01N33/53 ,  G01N33/558 ,  G01N33/66 ,  G01N33/68

CPC classification number: G01N33/6848 ,  G01N33/66 ,  Y10T436/24 ,  Y10T436/25375 ,  Y10T436/255

Abstract: A method is disclosed which provides a high throughput method for assigning plausible functions to unknown sequence entries in a particular database. The method was used herein to identify lectin proteins which can be found in specific tissues of the rice plant.

Abstract translation: 公开了一种提供用于将合理功能分配给特定数据库中的未知序列条目的高吞吐量方法的方法。 该方法用于鉴定可在水稻植物的特定组织中发现的凝集素蛋白质。