MEDIUM COMPOSITION INCLUDING ETHANOL FOR PRODUCTION OF 2,3-BUTANEDIOL FROM SYNTHETIC GAS AND 2,3-BUTANEDIOL PRODUCTION METHOD USING SAME

    公开(公告)号:US20230383317A1

    公开(公告)日:2023-11-30

    申请号:US18249379

    申请日:2020-11-24

    IPC分类号: C12P7/16 C12N1/20

    CPC分类号: C12P7/16 C12N1/20

    摘要: The present invention relates to a composition for producing 2,3-butanediol by using ethanol and synthetic gas and a 2,3-butanediol production method. A composition according to an aspect of the present invention includes ethanol as an active ingredient. In the case where a 2,3-butanediol production strain is inoculated into a medium containing the composition and cultured and a synthetic gas is added thereto, 2,3-butanediol, which is a biofuel and a chemical substance, can be economically produced with ethanol serving as a substrate. In the metabolism process, the carbon flux can be concentrated on the production route of the target material, whereby the composition has an excellent effect of increasing the production efficiency of 2,3-butanediol and enhancing the productivity of 2,3-butanediol by controlling only fermentation conditions such as amounts of the synthetic gas or ethanol and stirring speeds of the medium.

    DILUTION SOLUTION FOR MEASURING FREE STEROIDS IN BLOOD AND METHOD FOR MEASURING STEROID USING THE SAME

    公开(公告)号:US20230314292A1

    公开(公告)日:2023-10-05

    申请号:US18078328

    申请日:2022-12-09

    摘要: The present invention relates to a diluted solution for measuring free steroids in blood and a method for measuring free steroids using the same, and more particularly, to a method for preparing a diluted blood sample for measuring free steroids in blood and a composition for blood sample dilution solution.
    In the method for preparing a diluted blood sample for measuring free steroids in blood of the present invention, it was confirmed that physiologically active free cortisol could be evaluated with the cortisol enzyme immunoreaction detection kit without additional sample pretreatment by removing cortisol which is present by binding to excess albumin and cortisol-specific corticosteroid-binding globulin (CBG) contained in serum using a protein precipitation and neutralizing agent. In addition, by selectively removing the protein in the blood sample, it is possible to measure low molecular weight compounds, including steroids, which are strongly bound to proteins in the blood, such as cortisol, in free form.
    Therefore, the method of the present invention can be applied simply and effectively to medical sites requiring blood tests for disease diagnosis, monitoring of free cortisol before and after surgery, and emergency patients.