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公开(公告)号：US07229795B2
公开(公告)日：2007-06-12
申请号：US10787267
申请日：2004-02-25
Applicant: Veronique A. Dartois , James A. Hoch , Fernando Valle , Manoj Kumar
Inventor： Veronique A. Dartois , James A. Hoch , Fernando Valle , Manoj Kumar
CPC classification number: C07K14/26
Abstract: The invention provides isolated nucleic acid molecules encoding polypeptides having 2,5-DKG permease activity, and oligonucleotides therefrom. The isolated nucleic acid molecules can be expressed in appropriate bacterial cells to enhance the production of 2-KLG, which can subsequently be converted to ascorbic acid. Further provided are isolated polypeptides having 2,5-DKG permease activity, immunogenic peptides therefrom, and antibodies specific therefor. The invention also provides methods of identifying novel 2,5-DKG permeases.
Abstract translation: 本发明提供了编码具有2,5-DKG通透酶活性的多肽的分离的核酸分子及其寡核苷酸。 分离的核酸分子可以在适当的细菌细胞中表达以增强2-KLG的产生，随后将其转化为抗坏血酸。 还提供了具有2,5-DKG通透酶活性的分离的多肽，其免疫原性肽及其特异的抗体。 本发明还提供鉴定新型2,5-DKG通透酶的方法。
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公开(公告)号：US20060177915A1
公开(公告)日：2006-08-10
申请号：US11282498
申请日：2005-11-18
Applicant: Ramesh Nair , Mark Payne , Donald Trimbur , Fernando Valle
Inventor： Ramesh Nair , Mark Payne , Donald Trimbur , Fernando Valle
CPC classification number: C12P7/20
Abstract: Recombinant organisms are provided comprising genes encoding a glycerol-3-phosphate dehydrogenase and/or a glycerol-3-phosphatase activity useful for the production of glycerol from a variety of carbon substrates. The organisms further contain disruptions in the endogenous genes encoding proteins having glycerol kinase and glycerol dehydrogenase activities.
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公开(公告)号：US20060068477A1
公开(公告)日：2006-03-30
申请号：US11282000
申请日：2005-11-17
Applicant: Timothy Dodge , Fernando Valle
Inventor： Timothy Dodge , Fernando Valle
Abstract: The invention provides methods and host cells for the production of ascorbic acid intermediates. The invention also provides host cells having a modification in a polynucleotide that uncouples the catabolic pathway from the oxidative pathway by deleting the encoding for an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a polynucleotide that has deleted the encoding for endogenous enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such host cells are used for the production of products, such as, ascorbic acid intermediates. Nucleic acid and amino acid sequences with inactivated enzymatic activity which phosphorylates D-glucose at its 6th carbon and inactivated enzymatic activity which phosphorylates D-gluconate at its 6th carbon are provided.
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44.发明申请Method of creating a library of bacterial clones with varying levels of gene expression 审中-公开创建具有不同基因表达水平的细菌克隆文库的方法公开(公告)号：US20060014146A1
公开(公告)日：2006-01-19
申请号：US10511043
申请日：2003-04-18
Applicant: Philippe Soucaille , Marguerite Cervin , Fernando Valle
Inventor： Philippe Soucaille , Marguerite Cervin , Fernando Valle
CPC classification number: C12N15/1082 , C12Q1/689
Abstract: The present invention relates to a method of creating DNA libraries that include an artificial promoter library and/or a modified ribosome binding site library and transforming bacterial host cells with the library to obtain a population of bacterial clones having a range of expression levels for a chromosomal gene of interest.
Abstract translation: 本发明涉及一种创建包含人源启动子文库和/或修饰的核糖体结合位点文库的DNA文库的方法，并用文库转化细菌宿主细胞，以获得具有染色体表达水平范围的细菌克隆群 感兴趣的基因
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公开(公告)号：US20050079617A1
公开(公告)日：2005-04-14
申请号：US10728337
申请日：2003-12-03
Applicant: Marguerite Cervin , Philippe Soucaille , Fernando Valle , Gregory Whited
Inventor： Marguerite Cervin , Philippe Soucaille , Fernando Valle , Gregory Whited
Abstract: A method is disclosed for restoring a Glu+ phenotype to a PTS−/Glu− bacterial cell which was originally capable of utilizing a phosphotransferase transport system (PTS) for carbohydrate transport. Bacterial cells comprising the Glu+ phenotype have modified endogenous chromosomal regulatory regions which are operably linked to polynucleotides encoding galactose permeases and glucokinases.
Abstract translation: 公开了一种用于将Glu +表型恢复到最初能够利用磷酸转移酶转运系统（PTS）用于碳水化合物转运的PTS 细菌细胞的方法。 包含Glu +表型的细菌细胞具有修饰的内源性染色体调节区，其可操作地连接到编码半乳糖通透酶和葡萄糖激酶的多核苷酸。
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Patent Agency Ranking
公开(公告)号：US20050032088A1
公开(公告)日：2005-02-10
申请号：US10787267
申请日：2004-02-25
Applicant: Veronique Dartois , James Hoch , Fernando Valle , Manoj Kumar
Inventor： Veronique Dartois , James Hoch , Fernando Valle , Manoj Kumar
CPC classification number: C07K14/26
Abstract: The invention provides isolated nucleic acid molecules encoding polypeptides having 2,5-DKG permease activity, and oligonucleotides therefrom. The isolated nucleic acid molecules can be expressed in appropriate bacterial cells to enhance the production of 2-KLG, which can subsequently be converted to ascorbic acid. Further provided are isolated polypeptides having 2,5-DKG permease activity, immunogenic peptides therefrom, and antibodies specific therefor. The invention also provides methods of identifying novel 2,5-DKG permeases.
Abstract translation: 本发明提供了编码具有2,5-DKG通透酶活性的多肽的分离的核酸分子及其寡核苷酸。 分离的核酸分子可以在适当的细菌细胞中表达以增强2-KLG的产生，随后将其转化为抗坏血酸。 还提供了具有2,5-DKG通透酶活性的分离的多肽，其免疫原性肽及其特异的抗体。 本发明还提供鉴定新型2,5-DKG通透酶的方法。
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47.发明授权Method of creating a library of bacterial clones with varying levels of gene expression 有权创建具有不同基因表达水平的细菌克隆文库的方法公开(公告)号：US09534217B2
公开(公告)日：2017-01-03
申请号：US13155290
申请日：2011-06-07
Applicant: Philippe Soucaille , Marguerite A. Cervin , Fernando Valle
Inventor： Philippe Soucaille , Marguerite A. Cervin , Fernando Valle
CPC classification number: C12N15/1086 , C40B40/08 , C40B50/06
Abstract: The present invention relates to a method of creating DNA libraries that include an artificial promoter library and/or a modified ribosome binding site library and transforming bacterial host cells with the library to obtain a population of bacterial clones having a range of expression levels for a chromosomal gene of interest.
Abstract translation: 本发明涉及一种创建包含人源启动子文库和/或修饰的核糖体结合位点文库的DNA文库的方法，并用文库转化细菌宿主细胞，以获得具有染色体表达水平范围的细菌克隆群 感兴趣的基因
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48.发明授权Production of fatty alcohols with fatty alcohol forming acyl-CoA reductases (FAR) 有权用脂肪醇形成酰基辅酶A还原酶（FAR）生产脂肪醇公开(公告)号：US08574877B2
公开(公告)日：2013-11-05
申请号：US13494709
申请日：2012-06-12
Applicant: Robert McDaniel , Behnaz Behrouzian , Louis Clark , Douglas A. Hattendorf , Fernando Valle
Inventor： Robert McDaniel , Behnaz Behrouzian , Louis Clark , Douglas A. Hattendorf , Fernando Valle
IPC: C12P7/42 , C12P21/06 , C12P21/04 , C12Q1/68 , C12Q1/26 , C12N9/02 , C12N1/20 , C12N15/00 , C07H21/04
CPC classification number: C12P7/04 , C10L1/026 , C10L1/04 , C10L1/19 , C12N9/0008 , C12P7/649 , Y02E50/13
Abstract: The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.
Abstract translation: 本公开涉及从包含编码异源脂肪酰辅酶A还原酶（FAR）酶的基因的重组宿主细胞产生脂肪醇的方法。 本公开还涉及来自海洋细菌，特别是海洋γ蛋白杆菌如马里他杆菌和海洋杆菌的FAR酶及其功能片段; 编码FAR酶的多核苷酸和载体以及包含其的宿主细胞。
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公开(公告)号：US08420759B2
公开(公告)日：2013-04-16
申请号：US12459399
申请日：2009-06-30
Applicant: Frank J. Feher , Gregory M. Whited , Gopal K. Chotani , Fernando Valle , Carol Fioresi , Karl J. Sanford , Joseph C. McAuliffe , Marguerite Cervin , Aaron S. Puhala , Andrei Miasnikov , Ilana S. Aldor
Inventor： Frank J. Feher , Gregory M. Whited , Gopal K. Chotani , Fernando Valle , Carol Fioresi , Karl J. Sanford , Joseph C. McAuliffe , Marguerite Cervin , Aaron S. Puhala , Andrei Miasnikov , Ilana S. Aldor
CPC classification number: C08F236/18 , C08F2/00 , C08F36/04 , C08F136/08 , C08F236/08 , C08F236/10 , C08F236/12 , C08L9/00 , C12P5/007 , Y10T436/24
Abstract: It has been found that certain cells in culture can convert more than about 0.002 percent of the carbon available in the cell culture medium into isoprene. These cells have a heterologous nucleic acid that (i) encodes an isoprene synthase polypeptide and (ii) is operably linked to a promoter. The isoprene produced in such a cultured medium can then be recovered and polymerized into synthetic rubbers and other useful polymeric materials. The synthetic isoprene containing polymers of this invention offer the benefit of being verifiable as to being derived from non-petrochemical based resources. They can also be analytically distinguished from rubbers that come from natural sources. The present invention more specifically discloses a polyisoprene polymer which is comprised of repeat units that are derived from isoprene monomer, wherein the polyisoprene polymer has δ13C value of greater than −22‰.
Abstract translation: 已经发现，培养物中的某些细胞可将大于约0.002％的细胞培养基中可用的碳转化为异戊二烯。 这些细胞具有异源核酸，其（i）编码异戊二烯合酶多肽，和（ii）可操作地连接于启动子。 然后可以回收在这种培养基中产生的异戊二烯并聚合成合成橡胶和其它有用的聚合物材料。 本发明的合成含异戊二烯的聚合物提供可证实为从非石化基础资源得到的益处。 它们也可以分析与来自天然来源的橡胶。 本发明更具体地公开了由衍生自异戊二烯单体的重复单元组成的聚异戊二烯聚合物，其中聚异戊二烯聚合物的δ13C值大于-22‰。
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50.发明申请PRODUCTION OF FATTY ALCOHOLS WITH FATTY ALCOHOL FORMING ACYL-COA REDUCTASES (FAR) 有权生产脂肪醇与脂肪醇形成ACYA-COA还原剂（FAR）公开(公告)号：US20130040352A1
公开(公告)日：2013-02-14
申请号：US13494709
申请日：2012-06-12
Applicant: Robert McDaniel , Behnaz Behrouzian , Louis Clark , Douglas Hattendorf , Fernando Valle
Inventor： Robert McDaniel , Behnaz Behrouzian , Louis Clark , Douglas Hattendorf , Fernando Valle
CPC classification number: C12P7/04 , C10L1/026 , C10L1/04 , C10L1/19 , C12N9/0008 , C12P7/649 , Y02E50/13
Abstract: The disclosure relates to methods of producing fatty alcohols from recombinant host cells comprising genes encoding heterologous fatty acyl-CoA reductase (FAR) enzymes. The disclosure further relates to FAR enzymes and functional fragments thereof derived from marine bacterium and particularly marine gamma proteobacterium such as Marinobacter and Oceanobacter; polynucleotides encoding the FAR enzymes and vectors and host cells comprising the same.
Abstract translation: 本公开涉及从包含编码异源脂肪酰辅酶A还原酶（FAR）酶的基因的重组宿主细胞产生脂肪醇的方法。 本公开还涉及来自海洋细菌，特别是海洋γ蛋白杆菌如马里他杆菌和海洋杆菌的FAR酶及其功能片段; 编码FAR酶的多核苷酸和载体以及包含其的宿主细胞。
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