公开(公告)号：US20160289735A1

公开(公告)日：2016-10-06

申请号：US14672358

申请日：2015-03-30

Applicant: General Electric Company

Inventor： Michael John Gerdes ,  John Richard Nelson ,  Patrick McCoy Spooner ,  Ralf Lenigk

IPC: C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6804 ,  C12N15/1003 ,  C12Q2522/101 ,  G01N33/689

Abstract: A method is provided herein, wherein the method of capturing a sperm deoxyribo nucleic acid (DNA) in a sample, comprises contacting a lysis solution to the sample comprising at least a sperm cell or a sperm cell lysate to lyse the sperm cell. The sperm cell or sperm cell lysate comprises a protamine-DNA complex. The method further comprises applying at least a protamine-specific antibody to the lysed sperm cell, wherein the protamine-specific antibody binds to the protamine-DNA complex of the lysed sperm cell to form an antibody-protamine-DNA complex. The antibody binding is followed by capturing the antibody-protamine-DNA complex; and isolating and detecting the sperm DNA from the captured antibody-protamine-DNA complex.

Abstract translation: 本文提供了一种方法，其中在样品中捕获精子脱氧核糖核酸（DNA）的方法包括将裂解液与包含至少精子细胞或精细胞裂解物的样品接触以裂解精子细胞。 精子细胞或精子细胞裂解物包含鱼精蛋白-DNA复合物。 该方法还包括向裂解的精子细胞施加至少一种鱼精蛋白特异性抗体，其中鱼精蛋白特异性抗体结合裂解的精细胞的鱼精蛋白-DNA复合物以形成抗体 - 鱼精蛋白-DNA复合物。 抗体结合后，捕获抗体 - 鱼精蛋白-DNA复合物; 并从捕获的抗体 - 鱼精蛋白-DNA复合物中分离和检测精子DNA。

公开(公告)号：US09063041B2

公开(公告)日：2015-06-23

申请号：US13690801

申请日：2012-11-30

Applicant: General Electric Company

Inventor： Christopher Michael Puleo ,  John Richard Nelson ,  Patrick McCoy Spooner ,  Ralf Lenigk

IPC: B01L3/00 ,  B01L7/00 ,  G01N1/30 ,  G01N1/40

CPC classification number: G01N1/30 ,  A01N1/0242 ,  A01N1/0284 ,  B01L3/5023 ,  B01L3/5088 ,  B01L7/00 ,  B01L2200/0678 ,  B01L2300/0825 ,  B01L2300/126 ,  B01L2300/1877 ,  G01N2001/4027 ,  Y10T436/2525

Abstract: A method of drying a biological sample disposed on a substrate is provided. The method comprises providing the substrate comprising a sample loading area and a heat source; activating the heat source for generating heat; heating the substrate at least above 65° C.; and drying the biological sample. A device for storing sample is also provided, wherein the device comprises a substrate for biological sample-storage; and a heating component that generates heat to maintain a temperature of at least above 65° C. The heating component may contain one or more reagents, wherein the reagents generate heat to maintain a temperature of at least above 65° C.

Abstract translation: 提供了一种干燥设置在基板上的生物样品的方法。 该方法包括提供包括样品加载区域和热源的基底; 激活热源产生热量; 将衬底加热至少高于65℃; 并干燥生物样品。 还提供了用于存储样品的装置，其中所述装置包括用于生物样品储存的基底; 以及产生热量以保持至少高于65℃的温度的加热组分。加热组分可以含有一种或多种试剂，其中试剂产生热量以保持至少高于65℃的温度。

公开(公告)号：US20150167052A1

公开(公告)日：2015-06-18

申请号：US14105204

申请日：2013-12-13

Applicant: General Electric Company

Inventor： Weston Blaine Griffin ,  Erin Jean Finehout ,  Ying Mao ,  Ralf Lenigk ,  Daniel Jason Erno

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/5055 ,  B01L2200/027 ,  B01L2200/0689 ,  B01L2300/043 ,  B01L2300/045 ,  B01L2300/0816 ,  B01L2300/1827 ,  B01L2400/0487

Abstract: A sample storage and extraction device is provided. The sample storage and extraction device includes a substrate frame and a substrate cover. The substrate frame includes a substrate region configured to receive a sample substrate. The sample storage and extraction device further includes a compression assembly configured to provide an isolation zone in a portion of the sample substrate. Moreover, the sample storage and extraction device includes a fluidic channel configured to flow elution fluid to the isolation zone.

Abstract translation: 提供样品储存和提取装置。 样品存储和提取装置包括基板框架和基板盖。 衬底框架包括被配置为接收样品衬底的衬底区域。 样品存储和提取装置还包括被配置成在样品基底的一部分中提供隔离区的压缩组件。 此外，样品储存和提取装置包括流体通道，其构造成将洗脱流体流动到隔离区。

公开(公告)号：US20150050654A1

公开(公告)日：2015-02-19

申请号：US13970315

申请日：2013-08-19

Applicant: General Electric Company

Inventor： Erin Jean Finehout ,  Christopher Michael Puleo ,  Ralf Lenigk ,  Jason M. Nichols ,  John Nelson

IPC: C12Q1/68

CPC classification number: C12Q1/6844 ,  C12Q1/6816 ,  C12Q1/6851 ,  C12Q2565/137 ,  C12Q2565/625 ,  C12Q2527/113 ,  C12Q2565/133 ,  C12Q2545/114

Abstract: The present disclosure relates to characterization of biological samples by amplification detection in a porous substrate. By way of example, a porous substrate may include amplification reagents configured to provide a signal when released during amplification. When a sample is applied, amplification occurs as a wavefront from the application point, and the time that the wavefront reaches a distance on the porous substrate is related to an initial concentration of the sample applied. By detecting the distance traveled by the amplification products at one or more time points, an initial concentration of the sample may be estimated.

Abstract translation: 本公开涉及通过多孔底物中的扩增检测来表征生物样品。 举例来说，多孔底物可以包括配置成在放大期间释放时提供信号的扩增试剂。 当应用样品时，从应用点开始发生波前放大，波前在多孔基底上达到距离的时间与施加样品的初始浓度有关。 通过在一个或多个时间点检测扩增产物行进的距离，可以估计样品的初始浓度。