公开(公告)号：US20150050654A1

公开(公告)日：2015-02-19

申请号：US13970315

申请日：2013-08-19

Applicant: General Electric Company

Inventor： Erin Jean Finehout ,  Christopher Michael Puleo ,  Ralf Lenigk ,  Jason M. Nichols ,  John Nelson

IPC: C12Q1/68

CPC classification number: C12Q1/6844 ,  C12Q1/6816 ,  C12Q1/6851 ,  C12Q2565/137 ,  C12Q2565/625 ,  C12Q2527/113 ,  C12Q2565/133 ,  C12Q2545/114

Abstract: The present disclosure relates to characterization of biological samples by amplification detection in a porous substrate. By way of example, a porous substrate may include amplification reagents configured to provide a signal when released during amplification. When a sample is applied, amplification occurs as a wavefront from the application point, and the time that the wavefront reaches a distance on the porous substrate is related to an initial concentration of the sample applied. By detecting the distance traveled by the amplification products at one or more time points, an initial concentration of the sample may be estimated.

Abstract translation: 本公开涉及通过多孔底物中的扩增检测来表征生物样品。 举例来说，多孔底物可以包括配置成在放大期间释放时提供信号的扩增试剂。 当应用样品时，从应用点开始发生波前放大，波前在多孔基底上达到距离的时间与施加样品的初始浓度有关。 通过在一个或多个时间点检测扩增产物行进的距离，可以估计样品的初始浓度。

公开(公告)号：US09714447B2

公开(公告)日：2017-07-25

申请号：US13970315

申请日：2013-08-19

Applicant: General Electric Company

Inventor： Erin Jean Finehout ,  Christopher Michael Puleo ,  Ralf Lenigk ,  Jason M. Nichols ,  John Nelson

IPC: C12Q1/68

CPC classification number: C12Q1/6844 ,  C12Q1/6816 ,  C12Q1/6851 ,  C12Q2565/137 ,  C12Q2565/625 ,  C12Q2527/113 ,  C12Q2565/133 ,  C12Q2545/114

Abstract: The present disclosure relates to characterization of biological samples by amplification detection in a porous substrate. By way of example, a porous substrate may include amplification reagents configured to provide a signal when released during amplification. When a sample is applied, amplification occurs as a wavefront from the application point, and the time that the wavefront reaches a distance on the porous substrate is related to an initial concentration of the sample applied. By detecting the distance traveled by the amplification products at one or more time points, an initial concentration of the sample may be estimated.