公开(公告)号：US20120065125A1

公开(公告)日：2012-03-15

申请号：US13229146

申请日：2011-09-09

申请人： Hua YU ,  Marcin KORTYLEWSKI ,  Richard JOVE ,  Piotr Marek SWIDERSKI ,  John J. ROSSI

发明人： Hua YU ,  Marcin KORTYLEWSKI ,  Richard JOVE ,  Piotr Marek SWIDERSKI ,  John J. ROSSI

IPC分类号： A61K31/713 ,  A61K31/7105 ,  A61P37/00 ,  A61P31/00 ,  A61P7/00 ,  A61K38/02 ,  C07H21/02 ,  A61P35/00

CPC分类号： C12N15/113 ,  A61K31/7105 ,  A61K31/713 ,  A61K47/549 ,  A61K47/55 ,  A61K47/61 ,  A61K47/64 ,  C07H21/02 ,  C07K14/47 ,  C12N15/1135

摘要： The present invention relates to methods and compositions for the treatment of diseases, including cancer, infectious diseases and autoimmune diseases. The present invention also relates to methods and compositions for improving immune function. More particularly, the present invention relates to multifunctional molecules that are capable of being delivered to cells of interest for the treatment of diseases and for the improvement in immune function.

摘要翻译： 本发明涉及用于治疗疾病的方法和组合物，包括癌症，感染性疾病和自身免疫性疾病。 本发明还涉及用于改善免疫功能的方法和组合物。 更具体地，本发明涉及能够递送到感兴趣的细胞用于治疗疾病和改善免疫功能的多功能分子。

公开(公告)号：US20110318838A1

公开(公告)日：2011-12-29

申请号：US13230088

申请日：2011-09-12

申请人： John J. Rossi ,  Jiehua Zhou

发明人： John J. Rossi ,  Jiehua Zhou

IPC分类号： C12N5/071 ,  C07H21/02

CPC分类号： C12N15/1132 ,  C12N15/115 ,  C12N2310/14 ,  C12N2310/16 ,  C12N2310/3519 ,  C12N2310/531 ,  C12N2310/533 ,  C12N2320/32

摘要： The present invention relates to compositions and methods for delivery of siRNA to specific cells or tissue. More particularly, the present invention relates to compositions and methods for cell type-specific delivery of anti-HIV siRNAs via fusion to an anti-gp120 aptamer.

公开(公告)号：US08017759B2

公开(公告)日：2011-09-13

申请号：US10629895

申请日：2003-07-30

申请人： John J. Rossi ,  Nan-Sook Lee

发明人： John J. Rossi ,  Nan-Sook Lee

IPC分类号： C07H21/04

CPC分类号： C07K14/005 ,  C12N15/111 ,  C12N15/1132 ,  C12N15/85 ,  C12N15/86 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2330/30 ,  C12N2710/10322

摘要： An adenoviral VA1 Pol III expression system for RNAi expression is provided.

摘要翻译： 提供了用于RNAi表达的腺病毒VA1 Pol III表达系统。

公开(公告)号：US20110071210A1

公开(公告)日：2011-03-24

申请号：US12879199

申请日：2010-09-10

申请人： Hua YU ,  Marcin KORTYLEWSKI ,  Richard JOVE ,  Piotr Marek SWIDERSKI ,  John J. ROSSI

发明人： Hua YU ,  Marcin KORTYLEWSKI ,  Richard JOVE ,  Piotr Marek SWIDERSKI ,  John J. ROSSI

IPC分类号： A61K48/00 ,  C07H21/04 ,  C07H21/02 ,  A61P31/00 ,  A61P37/06 ,  A61P35/00

CPC分类号： A61K31/7105 ,  A61K31/713 ,  A61K47/549 ,  C07H21/02

摘要： The present invention relates to methods and compositions for the treatment of diseases, including cancer, infectious diseases and autoimmune diseases. The present invention also relates to methods and compositions for improving immune function. More particularly, the present invention relates to multifunctional molecules that are capable of being delivered to cells of interest for the treatment of diseases and for the improvement in immune function.

摘要翻译： 本发明涉及用于治疗疾病的方法和组合物，包括癌症，感染性疾病和自身免疫性疾病。 本发明还涉及用于改善免疫功能的方法和组合物。 更具体地，本发明涉及能够递送到感兴趣的细胞用于治疗疾病和改善免疫功能的多功能分子。

公开(公告)号：US07820632B2

公开(公告)日：2010-10-26

申请号：US10365643

申请日：2003-02-13

申请人： John J. Rossi ,  Nan-Sook Lee

发明人： John J. Rossi ,  Nan-Sook Lee

IPC分类号： C12P19/34 ,  A61K31/70 ,  C07H21/02 ,  C12Q1/68

CPC分类号： C12Q1/6876 ,  C12N15/111 ,  C12N15/113 ,  C12N15/1132 ,  C12N2310/111 ,  C12N2310/14 ,  C12N2310/53 ,  C12N2330/30 ,  C12Q2600/178

摘要： Methods for producing interfering RNA molecules in mammalian cells are provided. Therapeutic uses for the expressed molecules, including inhibiting expression of HIV, are also provided.

摘要翻译： 提供了在哺乳动物细胞中产生干扰RNA分子的方法。 还提供了表达分子的治疗用途，包括抑制HIV的表达。

公开(公告)号：US20100003758A1

公开(公告)日：2010-01-07

申请号：US12549730

申请日：2009-08-28

申请人： John J. ROSSI ,  Mark A. BEHLKE ,  Dongho KIM

发明人： John J. ROSSI ,  Mark A. BEHLKE ,  Dongho KIM

IPC分类号： C12N15/87

CPC分类号： C12N15/113 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/33 ,  C12N2310/50 ,  C12N2310/51 ,  C12N2320/30 ,  C12N2320/50 ,  C12N2320/51 ,  C12N2330/30

摘要： The invention provides compositions and methods for selectively reducing the expression of a gene product from a desired target gene, as well as treating diseases caused by expression of the gene. The method involves introducing into the environment of a cell an amount of a double-stranded RNA (dsRNA) such that a sufficient portion of the dsRNA can enter the cytoplasm of the cell to cause a reduction in the expression of the target gene. The dsRNA has a first oligonucleotide sequence that is between 26 and about 30 nucleotides in length and a second oligonucleotide sequence that anneals to the first sequence under biological conditions. In addition, a region of one of the sequences of the dsRNA having a sequence length of from about 19 to about 23 nucleotides is complementary to a nucleotide sequence of the RNA produced from the target gene.

公开(公告)号：US20090018321A1

公开(公告)日：2009-01-15

申请号：US12137914

申请日：2008-06-12

申请人： John J. ROSSI ,  Mark A. BEHLKE ,  Dongho KIM

发明人： John J. ROSSI ,  Mark A. BEHLKE ,  Dongho KIM

IPC分类号： C07H21/02

CPC分类号： C12N15/113 ,  C12N15/111 ,  C12N2310/14 ,  C12N2310/33 ,  C12N2310/50 ,  C12N2310/51 ,  C12N2320/30 ,  C12N2320/50 ,  C12N2320/51 ,  C12N2330/30

摘要： The invention provides compositions and methods for selectively reducing the expression of a gene product from a desired target gene, as well as treating diseases caused by expression of the gene. The method involves introducing into the environment of a cell an amount of a double-stranded RNA (dsRNA) such that a sufficient portion of the dsRNA can enter the cytoplasm of the cell to cause a reduction in the expression of the target gene. The dsRNA has a first oligonucleotide sequence that is between 26 and about 30 nucleotides in length and a second oligonucleotide sequence that anneals to the first sequence under biological conditions. In addition, a region of one of the sequences of the dsRNA having a sequence length of from about 19 to about 23 nucleotides is complementary to a nucleotide sequence of the RNA produced from the target gene.

公开(公告)号：US20080242632A1

公开(公告)日：2008-10-02

申请号：US12021604

申请日：2008-01-29

申请人： John J. ROSSI ,  Ola SNOVE ,  Ali EHSANI ,  Pal SAETROM ,  Britta HOEHN

发明人： John J. ROSSI ,  Ola SNOVE ,  Ali EHSANI ,  Pal SAETROM ,  Britta HOEHN

IPC分类号： A61K31/7105 ,  C07H21/02 ,  A61P35/04 ,  A61P9/00 ,  A61P29/00 ,  A61P31/18

CPC分类号： G06F19/18 ,  C12N15/111 ,  C12N15/113 ,  C12N15/1132 ,  C12N15/1135 ,  C12N15/1138 ,  C12N2310/14 ,  C12N2310/51 ,  C12N2310/53 ,  C12N2320/30 ,  C40B30/02 ,  G06F19/22

摘要： The present invention relates to novel short interfering RNA (siRNA) molecules that are multi-targeted. More specifically, the present invention relates to siRNA molecules that target two or more sequences. In one embodiment, multi-targeting siRNA molecules are designed to incorporate features of siRNA molecules and features of micro-RNA (miRNA) molecules. In another embodiment, multi-targeting siRNA molecules are designed so that each strand is directed to separate targets.

摘要翻译： 本发明涉及多靶向的新型短干扰RNA（siRNA）分子。 更具体地，本发明涉及靶向两个或更多个序列的siRNA分子。 在一个实施方案中，多靶向siRNA分子被设计为结合siRNA分子的特征和微RNA（miRNA）分子的特征。 在另一个实施方案中，设计多靶向siRNA分子，使得每条链被引导到分离的靶。

公开(公告)号：US20080153162A1

公开(公告)日：2008-06-26

申请号：US11859306

申请日：2007-09-21

申请人： John J. ROSSI ,  Dongho KIM

发明人： John J. ROSSI ,  Dongho KIM

IPC分类号： C12N5/06

CPC分类号： C12N15/1131 ,  C12N15/111 ,  C12N15/113 ,  C12N15/1135 ,  C12N15/117 ,  C12N2310/14 ,  C12N2310/17 ,  C12N2310/18 ,  C12N2310/53 ,  C12N2320/30 ,  C12N2330/30 ,  C12N2330/50

摘要： Double-stranded and single-stranded RNA molecules, and their use in methods for inducing interferon are provided. The interferon induction provides anti-viral and other medically useful effects, such as anti-cancer effects. Also provided are methods for reducing or inhibiting interferon induction exhibited by such molecules, particularly siRNA and shRNA molecules produced in vitro.

摘要翻译： 提供双链和单链RNA分子及其在诱导干扰素诱导方法中的应用。 干扰素诱导提供抗病毒和其他医学上有用的效果，例如抗癌作用。 还提供了用于减少或抑制由这些分子表现的干扰素诱导的方法，特别是在体外产生的siRNA和shRNA分子。

公开(公告)号：US06562570B1

公开(公告)日：2003-05-13

申请号：US09536393

申请日：2000-03-28

申请人： John J. Rossi ,  Michaela Scherr ,  Arthur D. Riggs

发明人： John J. Rossi ,  Michaela Scherr ,  Arthur D. Riggs

IPC分类号： C12Q168

CPC分类号： C12N15/113 ,  C12N15/1096 ,  C12Q1/6811 ,  C12Q1/6846 ,  C12Q2521/501 ,  C12Q2521/131 ,  C12Q2521/107

摘要： A method for identifying sites on a target RNA which are accessible to pairing by antisense DNA, ribozymes or DNAzymes. Native or in vitro-synthesized target RNA is incubated with defined ODNs and RNase H, or with a random or semi-random ODN library and RNase H, or with defined ribozymes or DNAzymes, or with a semi-random ribozyme or DNAzyme library, in a cell extract containing endogenous RNA-binding proteins, or in a medium which mimics a cell extract due to presence of one or more RNA-binding proteins. Any antisense ODN, ribozyme or DNAzyme which is complementary to an accessisble site in the target RNA hybridizes to that site and the RNA is cleaved at that site. Reverse transcription can be used to generate a first strand DNA from the RNA cleavage product, and terminal deoxynucleotidyl transferase-dependent polymerase chain reaction (TDPCR) can be used to identify sites on target RNA to which antisense ODNs, ribozymes or DNAzymes have bound and cleavage has occurred.