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39 条记录 (耗时 0.031 秒)

    LIMITING YEAST-PRODUCED TREHALOSE IN FERMENTATION
    31.
    发明申请
    LIMITING YEAST-PRODUCED TREHALOSE IN FERMENTATION 审中-公开

    公开(公告)号：US20180320203A1

    公开(公告)日：2018-11-08

    申请号：US15773139

    申请日：2016-11-04

    申请人： Lallemand Hungary Liquidity Management LLC

    发明人： Charles F. Rice ,  Ryan Skinner ,  Trisha Barrett ,  Aaron Argyros

    IPC分类号： C12P7/20 ,  C12N9/24 ,  C12N15/81 ,  C12N1/18

    CPC分类号： C12P7/10 ,  C12N9/2402 ,  C12Y302/01028 ,  Y02E50/16

    摘要： The present disclosure relates to recombinant yeast host cells having (i) a first genetic modification for reducing the production of one or more native enzymes that function to produce glycerol or regulating glycerol synthesis and/or allowing the production of an heterologous glucoamylase and (ii) a second genetic modification for reducing the production of one or more native enzymes that function to produce trehalose or regulating trehalose synthesis and/or allowing the expression of an heterologous trehalase. The recombinant yeast host cells can be used to limit the production of (yeast-produced) trehalose (particularly extracellular trehalose) during fermentation and, in some embodiments, can increase the production of a fermentation product (such as, for example, ethanol).

    Compositions and Methods to Prevent and Treat Biofilms
    33.
    发明申请
    Compositions and Methods to Prevent and Treat Biofilms 审中-公开
    防止和治疗生物膜的组合物和方法

    公开(公告)号：US20120315260A1

    公开(公告)日：2012-12-13

    申请号：US13481787

    申请日：2012-05-26

    申请人： Svetlana A. Ivanova ,  Dennis W. Davis ,  Brad W. Arenz ,  Thomas K. Connellan

    发明人： Svetlana A. Ivanova ,  Dennis W. Davis ,  Brad W. Arenz ,  Thomas K. Connellan

    IPC分类号： A61K38/47 ,  A61P31/00 ,  B23P11/00 ,  A61L2/18 ,  A01N63/00 ,  A61K38/54 ,  C12N9/24

    CPC分类号： C12Y302/01028 ,  A01N63/00 ,  A61K8/66 ,  A61K38/47 ,  A61Q11/00 ,  C12N9/2405 ,  Y10T29/49826 ,  A01N63/02 ,  A01N25/34

    摘要： Compositions and methods to treat biofilms are disclosed based on the discovery of the role of the disaccharide trehalose in microbial biofilm development. In various embodiments to treat body-borne biofilms systemically and locally, the method includes administering trehalase, the enzyme which degrades trehalose, in combination with other saccharidases for an exposition time sufficient to adequately degrade the biofilm gel matrix at the site of the biofilm. The method also includes administering a combination of other enzymes such as proteolytic, fibrinolytic, and lipolytic enzymes to break down proteins and lipids present in the biofilm, and administering antimicrobials for the specific type(s) of infectious pathogen(s) underlying the biofilm. Additionally, methods are disclosed to address degradation of biofilms on medical device surfaces and biofilms present in industrial settings.

    摘要翻译： 基于发现二糖海藻糖在微生物生物膜发育中的作用，公开了治疗生物膜的组合物和方法。 在各种实施方案中，全身和局部地治疗体内生物膜，该方法包括施用海藻糖酶，该降解海藻糖的酶与其它糖酵解酶组合，以便足以在生物膜的部位充分降解生物膜凝胶基质的暴露时间。 该方法还包括施用其它酶如蛋白水解，纤维蛋白溶解和脂肪分解酶的组合以分解存在于生物膜中的蛋白质和脂质，以及为生物膜下面的感染性病原体的特定类型施用抗微生物剂。 此外，公开了解决在工业设置中存在的医疗装置表面和生物膜上的生物膜的降解的方法。

    Method for selecting genetically transformed cells
    35.
    发明授权
    Method for selecting genetically transformed cells 失效
    选择遗传转化细胞的方法

    公开(公告)号：US07449290B2

    公开(公告)日：2008-11-11

    申请号：US10494651

    申请日：2002-11-06

    申请人： Josephus Christianus Maria Smeekens ,  Henriette Schlüpmann

    发明人： Josephus Christianus Maria Smeekens ,  Henriette Schlüpmann

    IPC分类号： C12Q1/68

    CPC分类号： C12Y302/01028 ,  C12N9/2402 ,  C12N15/8209

    摘要： An environmental friendly and non toxic method for selection of transformed cells from a population consisting of transformed and non-transformed cells. The method comprises the following steps a) introducing into a cell at least one desired nucleotide sequence and at least one selection-nucleotide sequence to obtain a genetically transformed cell, wherein the selection-nucleotide sequence comprises a region which codes for a protein involved in the metabolizing of trehalose; b) placing a population with transformed and non-transformed cells into contact with trehalose and/or derivative thereof; and c) selecting the transformed cells from the population on the basis of the capacity of the transformed cells to metabolize the trehalose and/or derivative.

    摘要翻译： 用于从由转化和非转化细胞组成的群体中选择转化细胞的环境友好和无毒的方法。 该方法包括以下步骤：a）向细胞中加入至少一种所需的核苷酸序列和至少一种选择核苷酸序列以获得遗传转化的细胞，其中所述选择核苷酸序列包含编​​码参与所述核苷酸序列的蛋白质的区域 代谢海藻糖; b）将具有转化和未转化细胞的群体放置成与海藻糖和/或其衍生物接触; 和c）基于转化细胞的能力来代替海藻糖和/或衍生物从群体中选择转化的细胞。

    Frozen dough-resistant, practical baker's yeast
    39.
    发明授权
    Frozen dough-resistant, practical baker's yeast 失效
    冷冻面团，实用面包酵母

    公开(公告)号：US06410303B1

    公开(公告)日：2002-06-25

    申请号：US08969415

    申请日：1997-10-21

    申请人： Hiroyuki Takano ,  Akihiro Hino ,  Chie Iyo ,  Yasuo Suzuki ,  Ryoichi Nakajima

    发明人： Hiroyuki Takano ,  Akihiro Hino ,  Chie Iyo ,  Yasuo Suzuki ,  Ryoichi Nakajima

    IPC分类号： C12N120

    CPC分类号： C12R1/865 ,  A21D6/008 ,  C12N1/18 ,  C12N9/2402 ,  C12Y302/01028

    摘要： The invention provides a diploid or higher polyploid, practical baker's yeast with good frozen dough resistance. This is produced through mating with one or more NTH1 gene-disrupted, haploid yeasts as produced through gene manipulation of disrupting the NTH1 gene in a haploid yeast of which the diploid is practical baker's yeast. The reduction in the trehalose content of the yeast is significantly prevented even when used in frozen dough, and the frozen dough containing the yeast is well resistant to long-term freezing and storage.

    摘要翻译： 本发明提供了具有良好的冷冻面团抗性的二倍体或更高倍数，实用的面包酵母。 这是通过与通过在二倍体是实用的面包酵母的单倍体酵母中破坏NTH1基因的基因操作产生的一种或多种NTH1基因破坏的单倍体酵母交配而产生的。 酵母的海藻糖含量的降低甚至在用于冷冻面团时也被显着地防止，并且含有酵母的冷冻面团对长期冷冻和储存具有良好的抗性。