公开(公告)号：US20240043798A1

公开(公告)日：2024-02-08

申请号：US18207363

申请日：2023-06-08

申请人： Trailhead Biosystems Inc.

发明人： Nooshin AMINI

IPC分类号： C12N5/079

CPC分类号： C12N5/0622 ,  C12N2501/60 ,  C12N2501/105 ,  C12N2501/33 ,  C12N2501/135 ,  C12N2501/115

摘要： Methods for generating pre-oligodendrocyte progenitor cells (pre-OPCs), oligodendrocyte progenitor cells (OPCs) and pre-myelinating oligodendrocytes (preOLs) from human pluripotent stem cells are provided using chemically-defined culture media that allow for generation of pre-OPCs in as little as three days, SOX10+OLIG2+NKX2-2+OPCs in as little as twelve days and CD9+A2B5+O4+CNPase+preOLs in as little as eighteen days. Two alternative culture protocols for generating OPCs are provided. Culture media, isolated cell populations and kits are also provided.

公开(公告)号：US11891622B2

公开(公告)日：2024-02-06

申请号：US15539473

申请日：2015-12-30

申请人： Cell Cure Neurosciences Ltd.

发明人： Osnat Bohana-Kashtan ,  Lior Ann Rosenberg Belmaker ,  Ofer Wiser

IPC分类号： C12N5/079 ,  A61K35/30

CPC分类号： C12N5/0621 ,  A61K35/30 ,  C12N2500/02 ,  C12N2500/38 ,  C12N2501/115 ,  C12N2501/15 ,  C12N2501/16 ,  C12N2506/02 ,  C12N2533/52

摘要： A population of human polygonal RPE cells is disclosed. At least 95% of the cells thereof co-express premelanosome protein (PMEL17) and cellular retinaldehyde binding protein (CRALBP), wherein the trans-epithelial electrical resistance of the cells is greater than 100 ohms. Methods of generating same are also disclosed.

公开(公告)号：US20230399622A1

公开(公告)日：2023-12-14

申请号：US18032291

申请日：2021-10-18

申请人： FUNDACIÓ CENTRE DE REGULACIÓ GENÒMICA ,  INSTITUCIÓ CATALANA DE RECERCA I ESTUDIS AVANÇATS

发明人： Maria Pia COSMA ,  Martina PESARESI

IPC分类号： C12N5/0775 ,  C12N5/12 ,  C12N15/86 ,  A61K9/00 ,  C12N5/079 ,  A61P27/02

CPC分类号： C12N5/0663 ,  C12N5/12 ,  C12N15/86 ,  A61K9/0048 ,  C12N5/0622 ,  A61P27/02

摘要： A genetically-modified stem cell comprises an exogenous nucleic acid encoding a chemokine receptor. The exogenous nucleic acid is operably linked to at least one promoter and/or enhancer sequence for expression of the chemokine receptor in the genetically-modified stem cell. The chemokine receptor may be selected from Ccr5, Cxcr6, Ccr1, Cxcr2 and/or Ccr3. The genetical-ly-modified stem cell may be used in a method for the treatment of tissue damage and/or degenerative disease, such as an eye disease or disorder. A modified viral vector packaging a recombinant viral-based genome, for use in a method of treating a subject suffering from tissue damage and/or degenerative disease, such as an eye disease or disorder is also described. Methods for treating tissue damage and/or degenerative disease, such as eye diseases or disorders, and pharmaceutical compositions for use in such methods are also described.

公开(公告)号：US11840708B2

公开(公告)日：2023-12-12

申请号：US16091450

申请日：2017-04-04

申请人： Wisconsin Alumni Research Foundation ,  Cedars-Sinai Medical Center

发明人： Eric V. Shusta ,  Scott G. Canfield ,  Clive N. Svendsen ,  Sean P. Palecek ,  Gad D. Vatine

IPC分类号： C12N5/071 ,  C12N5/0793 ,  C12N5/079

CPC分类号： C12N5/0697 ,  C12N5/069 ,  C12N5/0619 ,  C12N5/0622 ,  C12N2501/11 ,  C12N2501/115 ,  C12N2502/081 ,  C12N2502/086 ,  C12N2502/28 ,  C12N2506/45 ,  C12N2533/90

摘要： A method of creating an isogenic multicellular blood-brain barrier model from iPSCs is disclosed.

公开(公告)号：US20230355576A1

公开(公告)日：2023-11-09

申请号：US17912638

申请日：2021-10-09

申请人： SOOCHOW UNIVERSITY

发明人： Yanxia MA ,  Shanwen WEI ,  Mingming ZOU ,  Li NI ,  Liyu ZHOU ,  Di LI ,  Yanwei YANG

IPC分类号： A61K31/385 ,  C12N5/079 ,  A61P25/00 ,  A61K35/30

CPC分类号： A61K31/385 ,  C12N5/0622 ,  A61P25/00 ,  A61K35/30

摘要： The present invention relates to use of slow-releasing hydrogen sulfide organic donor ADT-OH drugs in the differentiation of neural precursor cells. ADT-OH can induce more directed differentiation of neural precursor cells into neurons and oligodendrocytes and less differentiation into astrocytes, promote the axon growth of neurons, and reduce the death of neural precursor cells, thereby providing a direction for the transplantation of neural precursor cells to repair damaged nerves. ADT-OH will likely become a new drug target for the clinical treatment of nervous system diseases.

公开(公告)号：US20230332102A1

公开(公告)日：2023-10-19

申请号：US18021475

申请日：2021-08-17

申请人： TOSOH CORPORATION

发明人： Junpei KADOTA ,  Yu IMAIZUMI

IPC分类号： C12N5/079 ,  C12N13/00

CPC分类号： C12N5/0618 ,  C12N13/00 ,  C12N2533/54 ,  C12N2533/52 ,  C12N2506/45

摘要： A method for inducing differentiation of a pluripotent stem cell into an ectodermal cell, that includes coating a cell culture substrate having regions (A) and (B) below with Matrigel or the like, and seeding the pluripotent stem cell, (A) an island-shaped region having cell adhesiveness or the like, and an adjacent region (B) having no cell adhesiveness or the like; forming a pluripotent stem cell aggregate adhering onto the cell culture substrate by adherently culturing the seeded pluripotent stem cell in a feeder-free manner in an undifferentiated state maintenance medium; forming an embryoid body by culturing the formed pluripotent stem cell aggregate in a medium containing a differentiation-inducing factor in a state of adhering onto the cell culture substrate; and forming an ectodermal cell aggregate by culturing the formed embryoid body in a medium containing a differentiation-inducing factor in a state of adhering onto the cell culture substrate.

公开(公告)号：US11786559B2

公开(公告)日：2023-10-17

申请号：US16332634

申请日：2017-09-13

申请人： ANGIOCRINE BIOSCIENCE, INC.

发明人： Michael Daniel Ginsberg ,  Daniel Joseph Nolan ,  Liang Qiang

IPC分类号： C12N5/079 ,  C12N5/071 ,  C07K14/005 ,  C12N15/86 ,  A61K35/44 ,  A61K35/13 ,  A61K35/30 ,  A61L27/38 ,  C12N5/09

CPC分类号： A61K35/44 ,  A61K35/13 ,  A61K35/30 ,  A61L27/3808 ,  A61L27/3878 ,  A61L27/3886 ,  C07K14/005 ,  C12N5/069 ,  C12N5/0622 ,  C12N5/0693 ,  C12N5/0697 ,  C12N15/86 ,  C12N2502/086 ,  C12N2502/28 ,  C12N2710/10322

摘要： The present invention relates, in part, to blood-brain barrier-like tissues that comprise engineered E40RF1+ endothelial cells, and to various compositions and methods useful for making and using such blood-brain barrier-like tissues—both in vitro and in vivo.

公开(公告)号：US20230287341A1

公开(公告)日：2023-09-14

申请号：US18018371

申请日：2021-07-28

申请人： SIGILON THERAPEUTICS, INC.

发明人： Marissa R. Donovan ,  Elina Makino ,  Erika Pearson

IPC分类号： C12N5/079 ,  A61P3/00

CPC分类号： C12N5/0621 ,  A61P3/00 ,  C12N2510/02

摘要： Described herein are genetically modified cells derived from a human cell and which contain at least one exogenous transcription unit inserted into at least one of five genomic insertion sites located in Chromosomes 1, 2, 7 and X, as well as compositions, pharmaceutical preparations, and implantable devices comprising the genetically modified cells, and methods of using the same for preventing or treating a disease, disorder, or condition.

公开(公告)号：US20230277728A1

公开(公告)日：2023-09-07

申请号：US18127958

申请日：2023-03-29

申请人： Mayo Foundation for Medical Education and Research

发明人： Alan D. Marmorstein ,  Raymond Iezzi ,  Jarel K. Gandhi ,  Jose S. Pulido

IPC分类号： A61L27/38 ,  A61K35/30 ,  A61K35/36 ,  A61K38/36 ,  A61K38/48 ,  A61L27/22 ,  A61L27/34 ,  A61L27/36 ,  A61L27/52 ,  C12N5/079

CPC分类号： A61L27/3813 ,  A61K35/30 ,  A61K35/36 ,  A61K38/363 ,  A61K38/484 ,  A61L27/225 ,  A61L27/34 ,  A61L27/3687 ,  A61L27/3808 ,  A61L27/3839 ,  A61L27/52 ,  C12N5/0621 ,  C12Y304/21007 ,  A61L2420/02 ,  A61L2430/16 ,  C12N2501/734 ,  C12N2501/999 ,  C12N2506/45 ,  C12N2533/56 ,  C12N2533/90

摘要： This document provides methods and materials for performing retinal pigment epithelium transplantation. For example, methods and materials for using fibrin supports for retinal pigment epithelium transplantation are provided.

公开(公告)号：US11731098B2

公开(公告)日：2023-08-22

申请号：US17628754

申请日：2021-01-15

申请人： NANTONG UNIVERSITY

发明人： Xin Tang ,  Cheng Sun ,  Xiaokun Gu ,  Xiaosong Gu ,  Qianru He ,  Tianmei Qian ,  Wenfeng Su ,  Haoming Li

IPC分类号： B01J13/02 ,  C07K1/14 ,  C07K1/22 ,  C12N5/079

CPC分类号： B01J13/02 ,  C07K1/14 ,  C12N5/0618

摘要： Ag—Fe3O4 immunomagnetic microsphere contains poly-D-lysine modified on the surface and S100β and/or MBP antibody linked by an amide bond. The Ag—Fe3O4 immunomagnetic microsphere can specifically capturing peripheral nerve tissue-derived exosomes. When the microsphere is used to extract nerve tissue-derived exosomes, the extraction yield of exosomes per unit volume of nerve tissue is high, and the nerve specificity is strong.