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    Nucleotide sequences of moraxella catarrhalis genome
    22.
    发明申请
    Nucleotide sequences of moraxella catarrhalis genome 审中-公开
    莫斯莫氏卡他氏菌基因组的核苷酸序列

    公开(公告)号：US20040067554A1

    公开(公告)日：2004-04-08

    申请号：US10672787

    申请日：2003-09-26

    发明人： Robert E. Lagace ,  Chandra Patterson ,  Kim L. Berg

    IPC分类号： C12P021/06

    CPC分类号： C07K14/212

    摘要： The present invention provides the genomic sequences of a library of purified, polynucleotides, or their complements, comprising the genome of Moraxella catarrhalis. The invention also provides the identification of open reading frames contained within the polynucleotides of the library. The present invention further provides for the use of the polynucleotides, their complements or fragments, and proteins or portions thereof for identifying ligands and useful diagnostic and therapeutic compositions. In addition the invention provides for vectors, host cell sand methods for producing M. catarrhalis proteins or portions thereof.

    摘要翻译： 本发明提供纯化的多核苷酸或其补体的文库的基因组序列，其包含卡他莫拉菌的基因组。 本发明还提供包含在文库多核苷酸内的开放阅读框的鉴定。 本发明进一步提供多核苷酸，其互补序列或片段以及蛋白质或其部分用于鉴定配体和有用的诊断和治疗组合物的用途。 此外，本发明提供用于产生卡他莫拉菌蛋白质或其部分的载体，宿主细胞沙方法。

    SPECIFIC AND UNIVERSAL PROBES AND AMPLIFICATION PRIMERS TO RAPIDLY DETECT AND IDENTIFY COMMON BACTERIAL PATHOGENS AND ANTIBIOTIC RESISTANCE GENES FROM CLINICAL SPECIMENS FOR ROUTINE DIAGNOSIS IN MICROBIOLOGY LABORATORIES
    27.
    发明申请
    SPECIFIC AND UNIVERSAL PROBES AND AMPLIFICATION PRIMERS TO RAPIDLY DETECT AND IDENTIFY COMMON BACTERIAL PATHOGENS AND ANTIBIOTIC RESISTANCE GENES FROM CLINICAL SPECIMENS FOR ROUTINE DIAGNOSIS IN MICROBIOLOGY LABORATORIES 审中-公开

    公开(公告)号：US20020055101A1

    公开(公告)日：2002-05-09

    申请号：US09452599

    申请日：1999-12-01

    发明人： MICHEL G. BERGERON ,  MARC OUELLETTE ,  PAUL H. ROY

    IPC分类号： C12Q001/68 ,  C12P019/34

    CPC分类号： C07K14/315 ,  C07K14/195 ,  C07K14/21 ,  C07K14/212 ,  C07K14/245 ,  C07K14/26 ,  C07K14/285 ,  C07K14/31 ,  C07K14/3156 ,  C12Q1/689 ,  C12Q1/6895 ,  C12Q2600/16

    摘要： The present invention relates to DNA-based methods for universal bacterial detection, for specific detection of the common bacterial pathogens Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Proteus mirabilis, Streptococcus pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Staphylococcus saprophyticus, Streptococcus pyogenes, Haemophilus influenzae and Moraxella catarrhalis as well as for specific detection of commonly encountered and clinically relevant bacterial antibiotic resistance genes directly from clinical specimens or, alternatively, from a bacterial colony. The above bacterial species can account for as much as 80% of bacterial pathogens isolated in routine microbiology laboratories. The core of this invention consists primarily of the DNA sequences from all species-specific genomic DNA fragments selected by hybridization from genomic libraries or, alternatively, selected from data banks as well as any oligonucleotide sequences derived from these sequences which can be used as probes or amplification primers for PCR or any other nucleic acid amplification methods. This invention also includes DNA sequences from the selected clinically relevant antibiotic resistance genes. With these methods, bacteria can be detected (universal primers and/or probes) and identified (species-specific primers and/or probes) directly from the clinical specimens or from an isolated bacterial colony. Bacteria are further evaluated for their putative susceptibility to antibiotics by resistance gene detection (antibiotic resistance gene specific primers and/or probes). Diagnostic kits for the detection of the presence, for the bacterial identification of the above-mentioned bacterial species and for the detection of antibiotic resistance genes are also claimed. These kits for the rapid (one hour or less) and accurate diagnosis of bacterial infections and antibiotic resistance will gradually replace conventional methods currently used in clinical microbiology laboratories for routine diagnosis. They should provide tools to clinicians to help prescribe promptly optimal treatments when necessary. Consequently, these tests should contribute to saving human lives, rationalizing treatment, reducing the development of antibiotic resistance and avoid unnecessary hospitalizations.

    High molecular weight major outer membrane protein of moraxella
    28.
    发明授权
    High molecular weight major outer membrane protein of moraxella 失效
    moraxella的高分子量主要外膜蛋白

    公开(公告)号：US06335018B1

    公开(公告)日：2002-01-01

    申请号：US08431718

    申请日：1995-05-01

    申请人： Ken Sasaki ,  Robin E. Harkness ,  Michel H. Klein

    发明人： Ken Sasaki ,  Robin E. Harkness ,  Michel H. Klein

    IPC分类号： C07K500

    CPC分类号： C07K14/212 ,  A61K39/00 ,  C07K2319/02 ,  Y02A50/466 ,  Y10S530/806 ,  Y10S530/825

    摘要： An isolated and purified outer membrane protein of a Moraxella strain, particularly M. catarrhalis, has a molecular mass of about 200 kDa. The about 200 kDa outer membrane protein as well as nucleic acid molecules encoding the same are useful in diagnostic applications and immunogenic compositions, particularly for in vivo administration to a host to confer protection against disease caused by a bacterial pathogen that produces the about 200 kDa outer membrane protein or produces a protein capable of inducing antibodies in a host specifically reactive with the about 200 kDa outer membrane protein.

    摘要翻译： 分离和纯化的莫拉氏菌属菌株，特别是卡他莫拉氏菌的外膜蛋白具有约200kDa的分子量。 大约200kDa的外膜蛋白以及编码它们的核酸分子可用于诊断应用和免疫原性组合物，特别是用于体内施用于宿主以赋予产生约200kDa外部细菌病原体引起的疾病的保护 产生能够在与约200kDa外膜蛋白特异性反应的宿主中诱导抗体的蛋白质。

    Gene encoding outer membrane protein B1 of moraxella catarrhalis
    30.
    发明授权
    Gene encoding outer membrane protein B1 of moraxella catarrhalis 失效
    基因编码卡他莫拉菌的外膜蛋白B1

    公开(公告)号：US5972657A

    公开(公告)日：1999-10-26

    申请号：US949941

    申请日：1997-10-14

    申请人： Timothy F. Murphy ,  Sanjay Sethi

    发明人： Timothy F. Murphy ,  Sanjay Sethi

    IPC分类号： C07K14/21 ,  C12P21/06 ,  C07H21/04 ,  C12N15/00 ,  C12P21/04

    CPC分类号： C07K14/212

    摘要： Nucleotide sequences, derived from Moraxella catarrhalis, which encode one or more epitopes of outer membrane protein B1 are disclosed. Recombinant B1 protein or B1 peptides may be produce by culturing in a medium a host cell genetically engineered to contain and express a nucleotide sequence according to the present invention, and recovering the recombinant protein or peptide from the cultured host cell or culture medium. The nucleotide sequence of the present invention can also be used in molecular diagnostic assays for detecting M. catarrhalis genetic material, and in antigenic compositions for producing B1-specific amino acids.

    摘要翻译： 公开了编码外膜蛋白B1的一个或多个表位的来自卡他莫拉菌的核苷酸序列。 重组B1蛋白或B1肽可以通过在遗传工程改造成含有和表达本发明的核苷酸序列的宿主细胞的培养基中培养产生，并从培养的宿主细胞或培养基中回收重组蛋白或肽。 本发明的核苷酸序列也可以用于检测卡他莫拉菌遗传物质的分子诊断测定，以及用于产生B1特异性氨基酸的抗原组合物中。