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74 条记录 (耗时 0.03 秒)

    Expression of recombinant tetracycline efflux pumps for the production of lysine or lysine-derived products, and methods and applications thereof
    21.
    发明授权
    Expression of recombinant tetracycline efflux pumps for the production of lysine or lysine-derived products, and methods and applications thereof 有权

    公开(公告)号：US11098329B2

    公开(公告)日：2021-08-24

    申请号：US16514518

    申请日：2019-07-17

    申请人： CATHAY BIOTECH INC. ,  CIBT AMERICA INC.

    发明人： Howard Chou ,  Xiucai Liu

    IPC分类号： C12P13/08 ,  C07K14/35 ,  C07K14/36 ,  C12N9/88 ,  C07K14/245 ,  C12N15/70 ,  C12N15/77 ,  C12P13/00

    摘要： One aspect of the invention relates to a mutant polypeptide comprising the amino acid sequence of Escherichia coli tetracycline efflux pump A (TetA). Another aspect of the invention relates to a polynucleotide encoding a polypeptide of TetA, a fragment thereof, or a mutant thereof. Another aspect of the invention relates to a first expression plasmid vector comprising one or more first polynucleotides encoding a first polypeptide comprising a tetracycline efflux pump polypeptide, a fragment thereof or a mutant thereof, one or more second polynucleotides independently selected from the group consisting of a third polynucleotide encoding a third polypeptide comprising a lysine decarboxylase polypeptide, a fragment thereof or a mutant thereof, and a fourth polynucleotide encoding a fourth polypeptide comprising a lysine biosynthesis polypeptide, a fragment thereof or a mutant thereof. Another aspect of the invention relates to a transformant comprising one or more expression plasmid vectors as described herein in a host cell. Another aspect of the invention relates to a mutant host cell comprising one or more first, third or fourth polynucleotides as described herein integrated into a chromosome of a host cell. Another aspect of the invention relates to a method for producing a lysine comprising obtaining a transformant and/or mutant host cell as disclosed herein, culturing the transformant and/or mutant host cell under conditions effective for the expression of the lysine; and harvesting the lysine. Other aspects of the invention relate to methods for producing biobased cadaverine using the transformants disclosed herein, and biobased cadaverine prepared by the method disclosed herein, and polyamides formed using the biobased cadaverine as disclosed herein and compositions thereof.

    EXPRESSION OF KLEBSIELLA OXYTOCA POLYPEPTIDES INVOLVED IN LYSINE DECARBOXYLATION, AND METHODS AND APPLICATIONS THEREOF
    22.
    发明申请
    EXPRESSION OF KLEBSIELLA OXYTOCA POLYPEPTIDES INVOLVED IN LYSINE DECARBOXYLATION, AND METHODS AND APPLICATIONS THEREOF 审中-公开

    公开(公告)号：US20200157152A1

    公开(公告)日：2020-05-21

    申请号：US16738663

    申请日：2020-01-09

    申请人： CATHAY BIOTECH INC. ,  CIBT AMERICA INC.

    发明人： Howard CHOU ,  Naiqiang LI ,  Xiucai LIU

    IPC分类号： C07K14/195 ,  C12P21/02 ,  C12N15/70 ,  C12N5/10 ,  C12P13/00 ,  C12N9/88

    摘要： One aspect provided herein relates to lysine decarboxylase polypeptides comprising mutants of SEQ ID NO: 2 (i.e., mutants of Klebsiella oxytoca (K. oxytoca) Ldc) and/or fragments thereof. In certain embodiments, the mutants or fragments thereof may have at least about 95% sequence identity with SEQ ID NO: 2. Another aspect provided herein relates to a DNA polynucleotide comprising one or more lysine decarboxylase nucleotide sequences of mutants of SEQ ID NO: 1 (i.e., mutants of K. oxytoca ldc), fragments thereof, or fragments of SEQ ID NO: 1 (i.e., fragments of K. oxytoca ldc). In certain embodiments, the DNA polynucleotide as disclosed herein may encode one or more lysine decarboxylase polypeptides provided herein. In certain embodiments, the DNA polynucleotide as disclosed herein may encode SEQ ID NO: 2, mutants, and/or fragments thereof. In certain embodiments, the lysine decarboxylase nucleotide sequences provided herein may have at least 95% sequence identity with SEQ ID NO: 1 or SEQ ID NO: 3. Another aspect provided herein relates to expression vectors comprising the DNA polynucleotides described herein used for production of a lysine-derived product. Other aspects provided herein include transformants, mutant host cells, methods for the production of lysine decarboxylases, and methods for the production of a lysine-derived product.

    Decanedioic acid produced by microbial fermentation process and preparation method thereof
    24.
    发明授权
    Decanedioic acid produced by microbial fermentation process and preparation method thereof 有权

    公开(公告)号：US11802298B2

    公开(公告)日：2023-10-31

    申请号：US17082124

    申请日：2020-10-28

    申请人： Cathay Biotech Inc. ,  CIBT America Inc.

    发明人： Min Xu ,  Yingli Hao ,  Chen Yang ,  Naiqiang Li ,  Xiucai Liu

    IPC分类号： C12P7/64 ,  C12P7/6409 ,  C08G69/26 ,  C12P7/44 ,  C08G63/16 ,  C07C55/20

    CPC分类号： C12P7/6409 ,  C07C55/20 ,  C08G63/16 ,  C08G69/26 ,  C12P7/44

    摘要： The present invention provides a decanedioic acid produced by microbial fermentation process, in which the content of C10 aliphatic acid and C10 hydroxy aliphatic acid is maintained at a very low level. The present invention also provides a preparation method of the decanedioic acid and a polymer prepared by using the decanedioic acid as monomer. The decanedioic acid provided by the present invention is prepared by microbial fermentation process. The decanedioic acid product which is produced through the processes of microbial fermentation and separation has a higher purity, a higher thermal stability, and a lower impurity content. The decanedioic acid provided by the present invention could satisfy the requirements of high grade product of polyamide or polyester to produce polymer with excellent qualities. The preparation method of the decanedioic acid provided by the present invention which has many advantages of mild reaction conditions, environmental friendliness, high yield, and good product quality, may replace the chemical method to be used in industrial scale production of the decanedioic acid.

    POLYAMIDE 5X FIBER, PREPARATION METHOD THEREOF, AND USE THEREOF
    27.
    发明申请
    POLYAMIDE 5X FIBER, PREPARATION METHOD THEREOF, AND USE THEREOF 有权

    公开(公告)号：US20230051716A1

    公开(公告)日：2023-02-16

    申请号：US17758892

    申请日：2020-01-15

    申请人： CATHAY BIOTECH INC. ,  CIBT AMERICA INC.

    发明人： Chaoxu SUN ,  Xiucai LIU

    IPC分类号： C08G69/26 ,  D01F6/60 ,  D01D5/088 ,  D01D5/098

    摘要： The present application discloses a polyamide 5X fiber, wherein raw materials for producing the polyamide 5X fiber comprise at least 1,5-pentane diamine and a long carbon chain diacid; or comprises a polyamide 5X obtained by the polymerization of 1,5-pentane diamine and a long carbon chain diacid as monomers. The 1,5-pentane diamine or the long carbon chain diacid are produced from bio-based raw materials by a fermentation process or an enzymatic conversion process; and the long carbon chain diacid is at least one of C6-20 aliphatic long carbon chain diacids. The long carbon chain diacid is at least one of C6-20 aliphatic long carbon chain diacids. The polyamide 5X fiber includes pre-oriented yarns, drawn textured yarns, fully drawn yarns, staple fibers, industrial yarns, continuous bulked filaments and monofilaments, preferably pre-oriented yarns, drawn textured yarns and/or fully drawn yarns. The raw materials used for producing the polyamide 5X resin are prepared by biological processes, and are green materials. The polyamide 5X fiber has good mechanical properties, dimensional stability, softness, quick-drying performance and dyeing properties.