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公开(公告)号：US11225677B2
公开(公告)日：2022-01-18
申请号：US16503301
申请日：2019-07-03
发明人： Wenbo Liu , Min Xu , Chen Yang , Howard Chou , Xiucai Liu
摘要： The invention relates to a long-chain dibasic acid with low content of monobasic acid impurity and a production method thereof, in particular to the preparation of a long-chain dibasic acid producing strain by means of directed evolution and homologous recombination, and to the production of a long-chain dibasic acid with low content of monobasic acid impurity by fermentation of said strain. The invention relates to a mutated CYP52A12 gene, homologous gene or variant thereof, which, relative to GenBank Accession Number AY230498 and taking the first base upstream of the start codon ATG as −1, comprises a mutation. The invention relates to a strain comprising said mutated CYP52A12 gene, homologous gene or variant thereof wherein when the strain is fermented to produce a long-chain dibasic acid, the content of monobasic acid impurity in the fermentation product is significantly reduced.
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公开(公告)号：US11434510B2
公开(公告)日：2022-09-06
申请号：US16503309
申请日：2019-07-03
发明人： Wenbo Liu , Min Xu , Chen Yang , Howard Chou , Xiucai Liu
IPC分类号： C12P7/44 , C12N15/52 , C12N9/02 , C12N15/01 , C12P7/6409
摘要： The present invention relates to a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain, to the preparation of a long-chain dibasic acid producing strain by directed evolution of POX gene and homologous recombination, and to the production of a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain by using the strain. The present invention also relates to a strain containing a mutated promoter, wherein, when a long-chain dibasic acid is produced by fermentation of this strain, the content of the acid impurity of shorter carbon chain in the fermentation product is significantly reduced.
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3.发明授权公开(公告)号：US11136596B2
公开(公告)日：2021-10-05
申请号：US16503304
申请日：2019-07-03
发明人： Wenbo Liu , Min Xu , Chen Yang , Howard Chou , Xiucai Liu
摘要： The present invention relates to a long-chain dibasic acid with low content of hydroxyl acid impurity and a production method thereof, in particular to a method for producing a long-chain dibasic acid with low content of hydroxyl acid impurity by fermenting a long-chain dibasic acid producing strain prepared by homologous recombination method. The present invention relates to a recombinant long-chain dibasic acid producing microorganism, having increased alcohol dehydrogenase activity and optionally decreased acyl-CoA oxidase activity. The present invention also relates to a method of producing a long-chain dibasic acid by the recombinant long-chain dibasic acid producing microorganism and use thereof.
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公开(公告)号：US11753629B2
公开(公告)日：2023-09-12
申请号：US17373341
申请日：2021-07-12
发明人： Wenbo Liu , Min Xu , Howard Chou , Xiucai Liu
IPC分类号： C12N9/02 , C12P7/44 , C12N15/52 , C12P7/6409
CPC分类号： C12N9/0071 , C12N15/52 , C12P7/44 , C12N9/001 , C12P7/6409 , C12Y114/14
摘要： The invention is directed to a method of preparing a long chain dicarboxylic acid producing strain by using directed evolution and homologous recombination, a strain obtained by this method that is capable of producing a long chain dicarboxylic acid under an acidic condition and the use of the strain. In particular, the invention is directed to a method of preparing a long chain dicarboxylic acid producing strain by using directed evolution of CYP52A12 gene and homologous recombination, a strain obtained by this method that is capable of producing a long chain dicarboxylic acid under an acidic condition and the use of the strain.
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公开(公告)号：US12018311B2
公开(公告)日：2024-06-25
申请号：US17814972
申请日：2022-07-26
发明人： Wenbo Liu , Min Xu , Chen Yang , Howard Chou , Xiucai Liu
IPC分类号： C12P7/44 , C12N9/02 , C12N15/01 , C12N15/52 , C12P7/6409
CPC分类号： C12P7/44 , C12N15/52 , C12Y103/03006
摘要： The present invention relates to a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain, to the preparation of a long-chain dibasic acid producing strain by directed evolution of POX gene and homologous recombination, and to the production of a long-chain dibasic acid with low content of long-chain dibasic acid impurity of shorter carbon chain by using the strain. The present invention also relates to a strain containing a mutated promoter, wherein, when a long-chain dibasic acid is produced by fermentation of this strain, the content of the acid impurity of shorter carbon chain in the fermentation product is significantly reduced.
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公开(公告)号：US11091742B2
公开(公告)日：2021-08-17
申请号：US16229084
申请日：2018-12-21
发明人： Wenbo Liu , Min Xu , Howard Chou , Xiucai Liu
摘要： The invention relates to the directed evolution of CYP52A12 gene and the use thereof for the production of a dicarboxylic acid. In particular, it relates to a method of preparing a long chain dicarboxylic acid producing strain by using directed evolution and homologous recombination, a strain obtained by the method that is capable of producing a long chain dicarboxylic acid under an acidic condition and the use thereof. In particular, the invention relates to a method of preparing a long chain dicarboxylic acid producing strain by directed evolution of CYP52A12 gene and homologous recombination, a strain obtained by the method that is capable of producing a long chain dicarboxylic acid under an acidic condition and the use thereof. By directed evolution of CYP52A12 gene, one strain which has a base mutation at the promoter region of said gene and is capable of producing a long chain dicarboxylic acid under an acidic condition in a shortened fermentation time is screened out in the invention.
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公开(公告)号：US10982241B2
公开(公告)日：2021-04-20
申请号：US16826606
申请日：2020-03-23
发明人： Wenbo Liu , Min Xu , Chen Yang , Howard Chou , Xiucai Liu
摘要： The invention relates to a long-chain dibasic acid with low content of monobasic acid impurity and a production method thereof, in particular to the preparation of a long-chain dibasic acid producing strain by means of directed evolution and homologous recombination, and to the production of a long-chain dibasic acid with low content of monobasic acid impurity by fermentation of said strain. The invention relates to a mutated CYP52A12 gene, homologous gene or variant thereof, which, relative to GenBank Accession Number AY230498 and taking the first base upstream of the start codon ATG as −1, comprises a mutation. The invention relates to a strain comprising said mutated CYP52A12 gene, homologous gene or variant thereof wherein when the strain is fermented to produce a long-chain dibasic acid, the content of monobasic acid impurity in the fermentation product is significantly reduced.
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