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21 条记录 (耗时 0.019 秒)

    Expression of Klebsiella oxytoca polypeptides involved in lysine decarboxylation, and methods and applications thereof
    1.
    发明授权
    Expression of Klebsiella oxytoca polypeptides involved in lysine decarboxylation, and methods and applications thereof 有权

    公开(公告)号：US11261217B2

    公开(公告)日：2022-03-01

    申请号：US16738663

    申请日：2020-01-09

    申请人： CATHAY BIOTECH INC. ,  CIBT AMERICA INC.

    发明人： Howard Chou ,  Naiqiang Li ,  Xiucai Liu

    IPC分类号： C07K14/195 ,  C12N9/88 ,  C12P13/00 ,  C12N5/10 ,  C12N15/70 ,  C12P21/02

    摘要： One aspect provided herein relates to lysine decarboxylase polypeptides comprising mutants of SEQ ID NO: 2 (i.e., mutants of Klebsiella oxytoca (K. oxytoca) Ldc) and/or fragments thereof. In certain embodiments, the mutants or fragments thereof may have at least about 95% sequence identity with SEQ ID NO: 2. Another aspect provided herein relates to a DNA polynucleotide comprising one or more lysine decarboxylase nucleotide sequences of mutants of SEQ ID NO: 1 (i.e., mutants of K. oxytoca ldc), fragments thereof, or fragments of SEQ ID NO: 1 (i.e., fragments of K. oxytoca ldc). In certain embodiments, the DNA polynucleotide as disclosed herein may encode one or more lysine decarboxylase polypeptides provided herein. In certain embodiments, the DNA polynucleotide as disclosed herein may encode SEQ ID NO: 2, mutants, and/or fragments thereof. In certain embodiments, the lysine decarboxylase nucleotide sequences provided herein may have at least 95% sequence identity with SEQ ID NO: 1 or SEQ ID NO: 3. Another aspect provided herein relates to expression vectors comprising the DNA polynucleotides described herein used for production of a lysine-derived product. Other aspects provided herein include transformants, mutant host cells, methods for the production of lysine decarboxylases, and methods for the production of a lysine-derived product.

    Long-chain dibasic acid with low content of monobasic acid impurity and the production method thereof
    4.
    发明授权
    Long-chain dibasic acid with low content of monobasic acid impurity and the production method thereof 有权

    公开(公告)号：US11225677B2

    公开(公告)日：2022-01-18

    申请号：US16503301

    申请日：2019-07-03

    申请人： Cathay Biotech Inc. ,  CIBT America Inc.

    发明人： Wenbo Liu ,  Min Xu ,  Chen Yang ,  Howard Chou ,  Xiucai Liu

    IPC分类号： C12P7/64 ,  C12N9/02

    摘要： The invention relates to a long-chain dibasic acid with low content of monobasic acid impurity and a production method thereof, in particular to the preparation of a long-chain dibasic acid producing strain by means of directed evolution and homologous recombination, and to the production of a long-chain dibasic acid with low content of monobasic acid impurity by fermentation of said strain. The invention relates to a mutated CYP52A12 gene, homologous gene or variant thereof, which, relative to GenBank Accession Number AY230498 and taking the first base upstream of the start codon ATG as −1, comprises a mutation. The invention relates to a strain comprising said mutated CYP52A12 gene, homologous gene or variant thereof wherein when the strain is fermented to produce a long-chain dibasic acid, the content of monobasic acid impurity in the fermentation product is significantly reduced.

    Expression of recombinant tetracycline efflux pumps for the production of lysine or lysine-derived products, and methods and applications thereof
    5.
    发明授权
    Expression of recombinant tetracycline efflux pumps for the production of lysine or lysine-derived products, and methods and applications thereof 有权

    公开(公告)号：US11098329B2

    公开(公告)日：2021-08-24

    申请号：US16514518

    申请日：2019-07-17

    申请人： CATHAY BIOTECH INC. ,  CIBT AMERICA INC.

    发明人： Howard Chou ,  Xiucai Liu

    IPC分类号： C12P13/08 ,  C07K14/35 ,  C07K14/36 ,  C12N9/88 ,  C07K14/245 ,  C12N15/70 ,  C12N15/77 ,  C12P13/00

    摘要： One aspect of the invention relates to a mutant polypeptide comprising the amino acid sequence of Escherichia coli tetracycline efflux pump A (TetA). Another aspect of the invention relates to a polynucleotide encoding a polypeptide of TetA, a fragment thereof, or a mutant thereof. Another aspect of the invention relates to a first expression plasmid vector comprising one or more first polynucleotides encoding a first polypeptide comprising a tetracycline efflux pump polypeptide, a fragment thereof or a mutant thereof, one or more second polynucleotides independently selected from the group consisting of a third polynucleotide encoding a third polypeptide comprising a lysine decarboxylase polypeptide, a fragment thereof or a mutant thereof, and a fourth polynucleotide encoding a fourth polypeptide comprising a lysine biosynthesis polypeptide, a fragment thereof or a mutant thereof. Another aspect of the invention relates to a transformant comprising one or more expression plasmid vectors as described herein in a host cell. Another aspect of the invention relates to a mutant host cell comprising one or more first, third or fourth polynucleotides as described herein integrated into a chromosome of a host cell. Another aspect of the invention relates to a method for producing a lysine comprising obtaining a transformant and/or mutant host cell as disclosed herein, culturing the transformant and/or mutant host cell under conditions effective for the expression of the lysine; and harvesting the lysine. Other aspects of the invention relate to methods for producing biobased cadaverine using the transformants disclosed herein, and biobased cadaverine prepared by the method disclosed herein, and polyamides formed using the biobased cadaverine as disclosed herein and compositions thereof.