NOVEL MACADAMIA ALLERGEN
    14.
    发明申请

    公开(公告)号:US20170209566A1

    公开(公告)日:2017-07-27

    申请号:US15414380

    申请日:2017-01-24

    IPC分类号: A61K39/35 C07K16/16 G01N33/68

    摘要: Provided is a polypeptide comprising a macadamia protein running in a 2D SDS PAGE at pH 6.3 to 8.7 and 53 to 67 kDa or a protein running in a 2D SDS PAGE at pH 6.5 to 7.9 and 20 to 25 kDa or an antigenic variant thereof, and a tag for detection and/or purification, a polypeptide fused with the macadamia protein, or the macadamia protein is modified by glycosylation, phosphorylation, acetylation, decarboxylation, citrullination, or hydroxylation.

    Heavy metal reduction in planta
    15.
    发明授权
    Heavy metal reduction in planta 有权
    植物重金属减少

    公开(公告)号:US09528118B2

    公开(公告)日:2016-12-27

    申请号:US13819063

    申请日:2011-08-31

    申请人: Lucien Bovet

    发明人: Lucien Bovet

    摘要: There is described a mutant, non-naturally occurring or transgenic plant or plant cell comprising (a) a polynucleotide selected from the group consisting of: (i) a polynucleotide comprising, consisting or consisting essentially of a sequence having at least 71% sequence identity to SEQ ID NOs: 1, 2, 27, 28 or 29 or 51; or (ii) a polynucleotide comprising, consisting or consisting essentially of a sequence having at least 65% sequence identity to any of SEQ ID NOs: 3 to 23 or 30 to 50; or (iii) a polynucleotide encoding a NtMRP polypeptide comprising, consisting or consisting essentially of a sequence having at least 65% sequence identity to any of SEQ ID NOs. 24 to 26 or 52, and wherein the polypeptide has heavy metal transporter activity; or (b) a polynucleotide construct of at least 15 contiguous nucleotides in length that is at least 65% identical to a region of any of SEQ ID NOs: 1 to 23 or 27 to 51; or (c) a double-stranded RNA comprising at least two sequences that are at least partially complementary to each other and wherein a sense strand comprises a first sequence and an antisense strand comprises a second sequence and wherein at least one of the sequences comprises at least 10 contiguous nucleotides of NtMRP RNA; or (d) an expression vector comprising the polynucleotide as set forth in (i), (ii) or (iii) or the polynucleotide construct as set forth in (b).

    摘要翻译: 描述了突变体,非天然存在或转基因植物或植物细胞,其包含(a)选自以下的多核苷酸:(i)多核苷酸,其包含,由或基本上由具有至少71%序列同一性 至SEQ ID NO:1,2,27,28或29或51; 或(ii)多核苷酸,其包含与SEQ ID NO:3至23或30至50中任一个具有至少65%序列同一性的序列,或由其组成或基本上由SEQ ID NO: 或(iii)编码NtMRP多肽的多核苷酸,其包含与SEQ ID NO中任一个具有至少65%序列同一性的序列,或者基本上由其组成。 24至26或52,并且其中所述多肽具有重金属转运蛋白活性; 或(b)与SEQ ID NO:1至23或27至51中任一个区域至少65%相同的至少15个连续核苷酸长度的多核苷酸构建体; 或(c)包含至少两个彼此至少部分互补的序列的双链RNA,其中有义链包含第一序列,反义链包含第二序列,并且其中至少一个序列包含 NtMRP RNA的最少10个连续核苷酸; 或(d)包含如(i),(ii)或(iii)所述的多核苷酸或(b)所述的多核苷酸构建体的表达载体。