公开(公告)号：US20240168036A1

公开(公告)日：2024-05-23

申请号：US18526020

申请日：2023-12-01

Applicant: GENENTECH, INC. ,  HOFFMANN-LA ROCHE INC.

Inventor： Jia Guo ,  Regina Kufer ,  Delia Li ,  Feng Yang ,  Inn Huam Yuk ,  Jinhua Zhang ,  Lance J. Cadang ,  Lu Dai

IPC: G01N33/68 ,  G01N1/40 ,  G01N30/72 ,  G01N30/88

CPC classification number: G01N33/6848 ,  G01N1/4044 ,  G01N30/7233 ,  G01N30/88 ,  G01N2030/8831

Abstract: The present disclosure relates to highly sensitive methods for determining the identity and quantity of one or more proteins in a sample. For example, the present disclosure provides methods for the highly sensitive identification and quantitation of residual host cell proteins in protein samples and can be adapted to identify and quantify proteins in either a targeted or a target-agnostic manner and can be modified to achieve a range of sensitivities appropriate for distinct use cases.

公开(公告)号：US20240140970A1

公开(公告)日：2024-05-02

申请号：US18003402

申请日：2021-11-26

Applicant: ASCENTAWITS PHARMACEUTICALS, LTD.

Inventor： Meizhen Ruan ,  Xiaohong Cai ,  Jianxin Duan ,  Donald T Jung ,  Anrong Li ,  Teng Meng ,  Lin Sun ,  Bing Li

IPC: C07F9/564 ,  B01D15/16 ,  B01D15/42 ,  C07B59/00 ,  G01N30/06 ,  G01N30/16 ,  G01N30/72

CPC classification number: C07F9/564 ,  B01D15/166 ,  B01D15/426 ,  C07B59/002 ,  G01N30/06 ,  G01N30/16 ,  G01N30/7233 ,  C07B2200/05 ,  G01N2030/027

Abstract: Described is a deuterated compound, and preparation method and use thereof The deuterated compound I has a structure as shown in Formula (I), wherein A is H or D, and at least one of eight As is D; M is H or an alkali metal, an alkaline earth metal, or an ammonium radical. The present invention provides use of the deuterated compound I as an internal standard for measuring the content of a metabolite II in a biological sample, wherein the metabolite II has a structure as shown in a Formula (II); wherein A is H; M is H or an alkali metal, an alkaline earth metal, or an ammonium radical. The present invention uses the deuterated compound I as an internal standard to quantitatively analysis the content of metabolite II at lower content in biological samples, which can not only meet the requirements for the lower limit of quantitation, but also meet the requirements for DMPK studies in clinical trials.

公开(公告)号：US20240133850A1

公开(公告)日：2024-04-25

申请号：US18381600

申请日：2023-10-17

Applicant: SHAANXI UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Chengyuan LIANG ,  Jinrong HU ,  Boxin ZHANG ,  Changhua KE ,  Shan YANG ,  Jingjing ZHOU ,  Jiaxuan LI ,  Qiufang XIE ,  Yue GE ,  Wenxue WANG

IPC: G01N30/86 ,  G01N30/14 ,  G01N30/72

CPC classification number: G01N30/8686 ,  G01N30/14 ,  G01N30/7233 ,  G01N30/8631 ,  G01N2030/027

Abstract: A fingerprint spectrum construction method for a new compound aloe capsule and a fingerprint spectrum includes: (1) taking powder of a new compound aloe capsule, carrying out reflux extraction, adding methanol, carrying out ultrasonic treatment, filtering, and taking a subsequent filtrate as a test solution; dissolving barbaloin, aloe-emodin, indirubin, tryptanthrin, aloesin, and β-sitosterol in methanol to obtain reference solutions; (2) injecting the test solution and the reference solutions into a high performance liquid chromatograph for gradient elution to obtain a chromatogram of the new compound aloe capsule and chromatograms of the reference solutions, respectively; and (3) labeling chemical components of peaks on the chromatogram of the new compound aloe capsule according to the chromatogram of the new compound aloe capsule and the chromatograms of the reference solutions to obtain a fingerprint spectrum of the new compound aloe capsule.

公开(公告)号：US11965865B2

公开(公告)日：2024-04-23

申请号：US17167503

申请日：2021-02-04

Applicant: Shimadzu Corporation

Inventor： Shin Fujita

IPC: G01N30/86 ,  G01N30/72 ,  G01N30/02

CPC classification number: G01N30/8637 ,  G01N30/7233 ,  G01N2030/027

Abstract: A peak analyzing method for separating overlapping peaks observed in a second signal waveform representing a relationship between a second parameter and a signal intensity into a plurality of individual peaks originating from different factors based on signal patterns observed in a dimension of a first parameter, the method including at least: performing singular value decomposition on an input matrix expressing three-dimensional data to be processed; estimating characteristic orientations within a space spanned by a plurality of basis vectors by performing a geometric analysis on a trajectory defined by a plurality of weighting vectors in an SVD projection space whose number of dimensions is equal to a lowered rank given by a singular value decomposition process; and deconvoluting signal waveforms in a first matrix of a dimension of the first parameter by a transformation matrix.

公开(公告)号：US11959897B2

公开(公告)日：2024-04-16

申请号：US17828858

申请日：2022-05-31

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Jikang Wu ,  Hongxia Wang ,  Haibo Qiu ,  Ning Li

IPC: G01N30/72 ,  G01N30/86 ,  H01J49/00 ,  H01J49/04 ,  H01J49/42 ,  G01N30/62

CPC classification number: G01N30/7233 ,  G01N30/8644 ,  H01J49/0031 ,  H01J49/0431 ,  H01J49/425 ,  G01N2030/628

Abstract: Disclosed are methods for improving compound detection and characterization. Methods for characterizing a sample are disclosed. The methods can include providing a sample to a liquid chromatography system capable of sample separation to generate sample components; analyzing sample components by multiplexed targeted selected ion monitoring (SIM) to generate an inclusion list; and performing iterative mass spectral data-dependent acquisition (DDA) from the inclusion list, to identify individual sample components thereby characterizing the sample. In one example, multiplexed targeted SIMs and iterative MS2 DDA acquisition is used to increase robust compound identification for cell culture medium analysis.

公开(公告)号：US11946937B2

公开(公告)日：2024-04-02

申请号：US16646289

申请日：2018-09-13

Applicant: Mayo Foundation for Medical Education and Research

Inventor： David L. Murray ,  David R. Barnidge

IPC: G01N33/68 ,  G01N30/72

CPC classification number: G01N33/6848 ,  G01N33/6854 ,  G01N33/6872 ,  G01N30/7233 ,  G01N30/7266 ,  G01N33/6857 ,  G01N2800/044 ,  G01N2800/102 ,  G01N2800/104 ,  G01N2800/347

Abstract: This document provides materials and methods for identifying and quantifying AIM polypeptides in a sample using mass spectrometry techniques. For example, methods of using mass spectrometry to identify and quantify AIM polypeptides in a serum sample are provided. In some cases, quantification of AIM polypeptides can be used to diagnose and/or treat patients having a disease or disorder characterized by altered (e.g., increased or decreased) AIM polypeptide levels.

公开(公告)号：US20240102977A1

公开(公告)日：2024-03-28

申请号：US18471808

申请日：2023-09-21

Applicant: Waters Technologies Corporation

Inventor： Johannes Vissers ,  Richard Denny

IPC: G01N30/86 ,  G01N30/72

CPC classification number: G01N30/8693 ,  G01N30/7233 ,  G01N30/8631 ,  G01N2030/027

Abstract: The present technology relates to a method and instrument for classifying a sample. The method collects raw data from an analytical instrument (e.g., LC-MS), quantifies consistency parameters from the raw chromatographic data (e.g., peak detection) by assigning criteria and determining probability ratios, and constructs a learning model quantitation step by weighing the presence, absence, or modulation of one or more factors of the one or more samples against a decision criterion for positivity. The method can then apply the learning model to an unknown sample to detect the presence, absence, or modulation of one or more factors in the unknown sample such as the presence, absence, or modulation of a disease state or multiple disease states.

公开(公告)号：US20240101591A1

公开(公告)日：2024-03-28

申请号：US18530764

申请日：2023-12-06

Applicant: CHARLES STURT UNIVERSITY

Inventor： Peter ANDERSON

IPC: C07H23/00 ,  G01N30/72 ,  G01N33/82

CPC classification number: C07H23/00 ,  G01N30/7233 ,  G01N33/82

Abstract: The invention discussed in this application relates to vitamin B12-based compounds that are useful as quantitative standards, particularly for the assessment of vitamin B12 deficiency.

公开(公告)号：US20240068995A1

公开(公告)日：2024-02-29

申请号：US18365467

申请日：2023-08-04

Applicant: Waters Technologies Corporation

Inventor： Robert Birdsall ,  Ying Qing Yu ,  Jacob Kellett

IPC: G01N30/08 ,  C12Q1/37 ,  G01N1/40 ,  G01N30/72 ,  G01N30/74 ,  G01N33/15 ,  G01N33/68

CPC classification number: G01N30/08 ,  C12Q1/37 ,  G01N1/4044 ,  G01N1/405 ,  G01N30/7233 ,  G01N30/74 ,  G01N33/15 ,  G01N33/6848

Abstract: The present technology relates to a method of analyzing a sample including an analyte. The method includes injecting the sample including the analyte into a mobile phase. The mobile phase includes a metal chelator additive having a concentration between about 1 ppm to about 10 ppm. The method also includes separating the analyte using liquid chromatography and analyzing the analyte using a mass spectrometer, an ultra-violet detector, or a combination thereof.

公开(公告)号：US20240036056A1

公开(公告)日：2024-02-01

申请号：US18374492

申请日：2023-09-28

Applicant: Regeneron Pharmaceuticals, Inc.

Inventor： Zhengqi Zhang ,  Yuetian Yan ,  Shunhai Wang ,  Jethro Prinston ,  Jennifer Nguyen

IPC: G01N33/68 ,  G01N30/72 ,  G01N30/74

CPC classification number: G01N33/6848 ,  G01N30/7233 ,  G01N30/74 ,  G01N2030/027

Abstract: The present invention relates to the field of protein characterization, and in particular to methods for identifying critical quality attributes of therapeutic proteins by implementing a workflow including using a competitive binding assay with insufficient capture molecule followed by LC-MS.