-
公开(公告)号：US20250067710A1
公开(公告)日：2025-02-27
申请号：US18628190
申请日：2024-04-05
Applicant: SHAANXI UNIVERSITY OF SCIENCE AND TECHNOLOGY
Inventor： Chengyuan LIANG , Changhua Ke , Yue Xing , Jingjing Zhou , Jinrong Hu , Boxin Zhang , Yunfei Zhang , Bingxing Zhang , Wen Wang , Fei Li , Yifan Li
Abstract: The present invention provides a method for constructing a fingerprint of a Xin Su Ning capsule and a fingerprint. The method includes: S1: taking contents of Xin Su Ning capsules of different batches, adding a methanol aqueous solution, and performing ultrasonic extraction to obtain test article solutions; S2: injecting the test article solutions into a high performance liquid chromatograph, performing gradient elution, performing chromatographic analysis, and recording chromatograms from 0 to 140 min; S3: importing the chromatograms into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system to obtain a fingerprint; and S4: performing mass spectral analysis on the test article solutions, and determining chemical constituents of chromatographic peaks in the fingerprint according to results of the mass spectral analysis. The method for constructing a fingerprint of a Xin Su Ning capsule provided in the present invention has advantages of simple, stable and high precision and good reproducibility.
-
2.发明公开
公开(公告)号：US20240133850A1
公开(公告)日：2024-04-25
申请号：US18381600
申请日：2023-10-17
Applicant: SHAANXI UNIVERSITY OF SCIENCE AND TECHNOLOGY
Inventor： Chengyuan LIANG , Jinrong HU , Boxin ZHANG , Changhua KE , Shan YANG , Jingjing ZHOU , Jiaxuan LI , Qiufang XIE , Yue GE , Wenxue WANG
CPC classification number: G01N30/8686 , G01N30/14 , G01N30/7233 , G01N30/8631 , G01N2030/027
Abstract: A fingerprint spectrum construction method for a new compound aloe capsule and a fingerprint spectrum includes: (1) taking powder of a new compound aloe capsule, carrying out reflux extraction, adding methanol, carrying out ultrasonic treatment, filtering, and taking a subsequent filtrate as a test solution; dissolving barbaloin, aloe-emodin, indirubin, tryptanthrin, aloesin, and β-sitosterol in methanol to obtain reference solutions; (2) injecting the test solution and the reference solutions into a high performance liquid chromatograph for gradient elution to obtain a chromatogram of the new compound aloe capsule and chromatograms of the reference solutions, respectively; and (3) labeling chemical components of peaks on the chromatogram of the new compound aloe capsule according to the chromatogram of the new compound aloe capsule and the chromatograms of the reference solutions to obtain a fingerprint spectrum of the new compound aloe capsule.
-
3.发明公开
公开(公告)号：US20240230607A9
公开(公告)日：2024-07-11
申请号：US18381605
申请日：2024-01-14
Applicant: SHAANXI UNIVERSITY OF SCIENCE AND TECHNOLOGY
Inventor： Chengyuan LIANG , Changhua KE , Yanzi WANG , Yuting LIU , Xiuding YANG , Ying ZHOU , Jiaxuan LI
CPC classification number: G01N30/8686 , G01N30/74 , G01N30/8631 , G01N2001/2893 , G01N2030/027 , G01N2030/062
Abstract: A fingerprint spectrum construction method for Xihuang capsules and a fingerprint spectrum includes: S1: taking contents of a Xihuang capsule, adding a methanol-chloroform-phosphoric acid solution, and carrying out ultrasonic extraction to obtain a Xihuang capsule test solution; S2: dissolving cholic acid, hyodeoxycholic acid, deoxycholic acid, bilirubin, muscone, and myrrhone in ethanol to obtain a mixed standard solution 1; dissolving quassin, 11-carbonyl-β-boswellic acid, 11-carbonyl-β-acetyl-boswellic acid, acetyl-11α-methoxy-β-boswellic acid, and sandaracopimaric acid in methanol to obtain a mixed standard solution 2; S3: respectively carrying out chromatography on the Xihuang capsule test solution and the mixed standard solutions 1 and 2, and recording corresponding chromatograms; and S4: constructing a fingerprint spectrum of the Xihuang capsule according to the chromatogram of the Xihuang capsule test solution and the chromatograms of the mixed standard solutions 1 and 2. The method can accurately, clearly and objectively evaluate the quality of Xihuang capsules.
-
4.发明公开
公开(公告)号：US20240230606A9
公开(公告)日：2024-07-11
申请号：US18381600
申请日：2023-10-18
Applicant: SHAANXI UNIVERSITY OF SCIENCE AND TECHNOLOGY
Inventor： Chengyuan LIANG , Jinrong HU , Boxin ZHANG , Changhua KE , Shan YANG , Jingjing ZHOU , Jiaxuan LI , Qiufang XIE , Yue GE , Wenxue WANG
CPC classification number: G01N30/8686 , G01N30/14 , G01N30/7233 , G01N30/8631 , G01N2030/027
Abstract: A fingerprint spectrum construction method for a new compound aloe capsule and a fingerprint spectrum includes: (1) taking powder of a new compound aloe capsule, carrying out reflux extraction, adding methanol, carrying out ultrasonic treatment, filtering, and taking a subsequent filtrate as a test solution; dissolving barbaloin, aloe-emodin, indirubin, tryptanthrin, aloesin, and β-sitosterol in methanol to obtain reference solutions; (2) injecting the test solution and the reference solutions into a high performance liquid chromatograph for gradient elution to obtain a chromatogram of the new compound aloe capsule and chromatograms of the reference solutions, respectively; and (3) labeling chemical components of peaks on the chromatogram of the new compound aloe capsule according to the chromatogram of the new compound aloe capsule and the chromatograms of the reference solutions to obtain a fingerprint spectrum of the new compound aloe capsule.
-
5.发明公开
公开(公告)号：US20240228674A9
公开(公告)日：2024-07-11
申请号：US18381603
申请日：2023-10-18
Applicant: SHAANXI UNIVERSITY OF SCIENCE AND TECHNOLOGY
Inventor： Chengyuan LIANG , Boxin ZHANG , Changhua KE , Shan YANG , Jingjing ZHOU , Jinrong HU , Yuting LIU
IPC: C12P19/04
CPC classification number: C12P19/04
Abstract: The present invention provides a Ganoderma lucidum β-glucan extract, a preparation method and detection method therefor. The preparation method includes: S1: crushing fruiting bodies of Ganoderma lucidum, mixing the crushed fruiting bodies with a solvent, adding trypsin for enzymatic hydrolysis for 0.5-2 h, and separating to obtain a Ganoderma lucidum fruiting body filter residue; S2: mixing the Ganoderma lucidum fruiting body filter residue with an alkali solution, carrying out ultrasonic extraction with heating, adjusting the pH value of the obtained filtrate to be neutral, and concentrating to obtain a crude Ganoderma lucidum polysaccharide; S3: carrying out alcohol precipitation on the crude Ganoderma lucidum polysaccharide, separating the obtained precipitate, and freeze-drying to obtain a crude Ganoderma lucidum β-glucan extract; and S4: purifying the crude Ganoderma lucidum β-glucan extract by glucose gel column chromatography to obtain a Ganoderma lucidum β-glucan extract.
-
6.发明公开
公开(公告)号：US20240133851A1
公开(公告)日：2024-04-25
申请号：US18381605
申请日：2024-01-14
Applicant: SHAANXI UNIVERSITY OF SCIENCE AND TECHNOLOGY
Inventor： Chengyuan LIANG , Changhua KE , Yanzi WANG , Yuting LIU , Xiuding YANG , Ying ZHOU , Jiaxuan LI
CPC classification number: G01N30/8686 , G01N30/74 , G01N30/8631 , G01N2001/2893 , G01N2030/027 , G01N2030/062
Abstract: A fingerprint spectrum construction method for Xihuang capsules and a fingerprint spectrum includes: S1: taking contents of a Xihuang capsule, adding a methanol-chloroform-phosphoric acid solution, and carrying out ultrasonic extraction to obtain a Xihuang capsule test solution; S2: dissolving cholic acid, hyodeoxycholic acid, deoxycholic acid, bilirubin, muscone, and myrrhone in ethanol to obtain a mixed standard solution 1; dissolving quassin, 11-carbonyl-β-boswellic acid, 11-carbonyl-β-acetyl-boswellic acid, acetyl-11α-methoxy-β-boswellic acid, and sandaracopimaric acid in methanol to obtain a mixed standard solution 2; S3: respectively carrying out chromatography on the Xihuang capsule test solution and the mixed standard solutions 1 and 2, and recording corresponding chromatograms; and S4: constructing a fingerprint spectrum of the Xihuang capsule according to the chromatogram of the Xihuang capsule test solution and the chromatograms of the mixed standard solutions 1 and 2. The method can accurately, clearly and objectively evaluate the quality of Xihuang capsules.
-
7.发明公开
公开(公告)号：US20240132928A1
公开(公告)日：2024-04-25
申请号：US18381603
申请日：2023-10-17
Applicant: SHAANXI UNIVERSITY OF SCIENCE AND TECHNOLOGY
Inventor： Chengyuan LIANG , Boxin ZHANG , Changhua KE , Shan YANG , Jingjing ZHOU , Jinrong HU , Yuting LIU
IPC: C12P19/04
CPC classification number: C12P19/04
Abstract: The present invention provides a Ganoderma lucidum β-glucan extract, a preparation method and detection method therefor. The preparation method includes: S1: crushing fruiting bodies of Ganoderma lucidum, mixing the crushed fruiting bodies with a solvent, adding trypsin for enzymatic hydrolysis for 0.5-2 h, and separating to obtain a Ganoderma lucidum fruiting body filter residue; S2: mixing the Ganoderma lucidum fruiting body filter residue with an alkali solution, carrying out ultrasonic extraction with heating, adjusting the pH value of the obtained filtrate to be neutral, and concentrating to obtain a crude Ganoderma lucidum polysaccharide; S3: carrying out alcohol precipitation on the crude Ganoderma lucidum polysaccharide, separating the obtained precipitate, and freeze-drying to obtain a crude Ganoderma lucidum β-glucan extract; and S4: purifying the crude Ganoderma lucidum β-glucan extract by glucose gel column chromatography to obtain a Ganoderma lucidum β-glucan extract.
