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公开(公告)号：US20110003353A1
公开(公告)日：2011-01-06
申请号：US12857480
申请日：2010-08-16
Applicant: Robert M. CALDWELL , M. Harunur Rashid , Fernando Valle
Inventor： Robert M. CALDWELL , M. Harunur Rashid , Fernando Valle
Abstract: The present invention concerns multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate, said complexes having a dehydrogenase subunit and a cytochrome C subunit. The invention further relates to polynucleotides coding for the multimeric complexes and methods of use thereof.
Abstract translation: 本发明涉及在碳底物的酶促转化中起作用的多聚体氧化还原酶复合物，所述复合物具有脱氢酶亚基和细胞色素C亚基。 本发明还涉及编码多聚体复合物的多核苷酸及其使用方法。
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公开(公告)号：US20090142843A1
公开(公告)日：2009-06-04
申请号：US11971341
申请日：2008-01-09
Applicant: Marguerite A. Cervin , Philippe Soucaille , Fernando Valle , Gregory M. Whited
Inventor： Marguerite A. Cervin , Philippe Soucaille , Fernando Valle , Gregory M. Whited
Abstract: A method is disclosed for restoring a Glu+ phenotype to a PTS−/Glu− bacterial cell which was originally capable of utilizing a phosphotransferase transport system (PTS) for carbohydrate transport. Bacterial cells comprising the Glu+ phenotype have modified endogenous chromosomal regulatory regions which are operably linked to polynucleotides encoding galactose permeases and glucokinases.
Abstract translation: 公开了一种将Glu +表型恢复到最初能够利用磷酸转移酶转运系统（PTS）用于碳水化合物转运的PTS- / Glu-细菌细胞的方法。 包含Glu +表型的细菌细胞具有修饰的内源性染色体调节区，其可操作地连接到编码半乳糖通透酶和葡糖激酶的多核苷酸。
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公开(公告)号：US20080254523A1
公开(公告)日：2008-10-16
申请号：US12038373
申请日：2008-02-27
Applicant: Timothy C. Dodge , Fernando Valle
Inventor： Timothy C. Dodge , Fernando Valle
IPC: C12P7/58
Abstract: The invention provides methods and host cells for the production of ascorbic acid intermediates. The invention also provides host cells having a modification in a polynucleotide that uncouples the catabolic pathway from the oxidative pathway by deleting the encoding for an endogenous enzymatic activity that phosphorylates D-glucose at its 6th carbon and/or a polynucleotide that has deleted the encoding for endogenous enzymatic activity that phosphorylates D-gluconate at its 6th carbon. Such host cells are used for the production of products, such as, ascorbic acid intermediates. Nucleic acid and amino acid sequences with inactivated enzymatic activity which phosphorylates D-glucose at its 6th carbon and inactivated enzymatic activity which phosphorylates D-gluconate at its 6th carbon are provided.
Abstract translation: 本发明提供了用于生产抗坏血酸中间体的方法和宿主细胞。 本发明还提供了在多核苷酸中具有修饰的宿主细胞，其通过缺失在其第6个碳上磷酸化D-葡萄糖的内源性酶活性和/或已经缺失编码的多核苷酸的编码来解离分解代谢途径从氧化途径 在其第6个碳上磷酸化D-葡萄糖酸的内生酶活性。 这样的宿主细胞用于生产产品，如抗坏血酸中间体。 提供具有灭活的酶活性的核酸和氨基酸序列，其在其第6个碳上磷酸化D-葡萄糖，并且在其第6个碳上磷酸化D-葡萄糖酸盐的失活的酶活性。
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14.发明授权Metabolically engineered bacterial strains having non-functional endogenous gluconate transporters 失效具有非功能性内源葡萄糖酸盐转运蛋白的代谢改造的细菌菌株公开(公告)号：US07419795B2
公开(公告)日：2008-09-02
申请号：US10842929
申请日：2004-05-10
Applicant: Timothy C. Dodge , Manoj Kumar , M. Harunur Rashid , Fernando Valle
Inventor： Timothy C. Dodge , Manoj Kumar , M. Harunur Rashid , Fernando Valle
IPC: G01N33/53
Abstract: The present invention relates to engineering metabolic pathways in bacterial host cells which results in enhanced carbon flow for the production of ascorbic acid (ASA) intermediates. In particular, the invention relates to increasing the production of ASA intermediates in bacterial cells by enhancing the availability of gluconate resulting from the inactivation of endogenous gluconate transporter genes.
Abstract translation: 本发明涉及在细菌宿主细胞中工程代谢途径，其导致用于生产抗坏血酸（ASA）中间体的增强的碳流。 特别地，本发明涉及通过增加由内源性葡萄糖酸转运蛋白基因失活产生的葡萄糖酸盐的可利用性来增加细菌细胞中ASA中间体的产生。
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公开(公告)号：US20060246562A1
公开(公告)日：2006-11-02
申请号：US11479194
申请日：2006-06-29
Applicant: Nigel Dunn-Coleman , Anthony Gatenby , Fernando Valle
Inventor： Nigel Dunn-Coleman , Anthony Gatenby , Fernando Valle
CPC classification number: C12N9/88 , C12P7/18 , C12Y402/0103
Abstract: Recombinant organisms are provided comprising genes encoding cob(II)alamin reductase, cob(I)alamin adenosyltransferase, glycerol dehydratase and 1,3-propanediol oxidoreductase activities useful for the production of 1,3-propanediol from a variety of carbon substrates.
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Patent Agency Ranking
16.发明申请Methods of creating modified promoters resulting in varying levels of gene expression 审中-公开产生修饰的启动子的方法导致不同程度的基因表达公开(公告)号：US20060057633A1
公开(公告)日：2006-03-16
申请号：US10505844
申请日：2003-04-18
Applicant: Marguerite Cervin , Fernando Valle
Inventor： Marguerite Cervin , Fernando Valle
CPC classification number: C12N15/1082 , C12N15/63 , C12N15/70 , C12N15/71 , C12N15/72 , C40B30/06 , C40B40/02 , C40B50/06
Abstract: The present invention relates to a method of creating promoter cassettes that include modified precursor promoters and transforming a population of bacterial host cells with a promoter library comprising the promoter cassettes resulting in bacterial clones having a range of expression levels of a gene of interest. The invention further relates to selecting a transformed bacterial host cell which has an optimum level of gene expression.
Abstract translation: 本发明涉及一种产生启动子盒的方法，其包括经修饰的前体启动子并用包含启动子盒的启动子文库转化细菌宿主细胞群，从而产生具有一定目的基因表达水平范围的细菌克隆。 本发明还涉及选择具有最佳基因表达水平的转化细菌宿主细胞。
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公开(公告)号：US06911322B2
公开(公告)日：2005-06-28
申请号：US10308368
申请日：2002-12-03
Applicant: Fernando Valle , Eugenio Ferrari
Inventor： Fernando Valle , Eugenio Ferrari
IPC: C12N15/09 , C12N1/21 , C12N9/10 , C12N9/12 , C12N9/14 , C12N9/16 , C12N9/20 , C12N9/26 , C12N9/42 , C12N9/54 , C12N9/56 , C12N9/90 , C12R1/07 , C12R1/08 , C12R1/09 , C12R1/10 , C12R1/125 , C07K14/00 , C07H21/04 , C12N15/00
CPC classification number: C12N9/54
Abstract: The present invention provides a mutant aprE promoter and methods for the production of a desired protein in a Bacillus host cell, which comprises the mutant aprE promoter. The present invention provides the sequence of a preferred aprE mutant promoter, which provided for a 100-fold increase in the production of a protein from Bacillus subtilis.
Abstract translation: 本发明提供突变体aprE启动子和在芽孢杆菌宿主细胞中产生所需蛋白质的方法，其包含突变体aprE启动子。 本发明提供了优选的aprE突变启动子的序列，其提供了从枯草芽孢杆菌产生蛋白质的100倍的增加。
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公开(公告)号：US20130078699A1
公开(公告)日：2013-03-28
申请号：US13600132
申请日：2012-08-30
Applicant: Marguerite A. CERVIN , Gopal K. Chotani , Frank J. Feher , Richard La Duca , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Aaron S. Puhala , Karl J. Sanford , Fernando Valle , Gregory M. Whited
Inventor： Marguerite A. CERVIN , Gopal K. Chotani , Frank J. Feher , Richard La Duca , Joseph C. McAuliffe , Andrei Miasnikov , Caroline M. Peres , Aaron S. Puhala , Karl J. Sanford , Fernando Valle , Gregory M. Whited
IPC: C12P5/00
CPC classification number: C12P5/007 , C12N9/1022 , C12N9/1205 , C12N9/1229 , C12N9/88 , C12N9/90 , C12N15/52 , C12Y203/01009 , C12Y207/01036 , C12Y401/01033 , C12Y402/03027 , C12Y503/03002 , Y02E50/343
Abstract: The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells.
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公开(公告)号：US08389214B2
公开(公告)日：2013-03-05
申请号：US11971341
申请日：2008-01-09
Applicant: Marguerite A. Cervin , Philippe Soucaille , Fernando Valle , Gregory M. Whited
Inventor： Marguerite A. Cervin , Philippe Soucaille , Fernando Valle , Gregory M. Whited
IPC: C12Q1/68
Abstract: A method is disclosed for restoring a Glu+ phenotype to a PTS−/Glu− bacterial cell which was originally capable of utilizing a phosphotransferase transport system (PTS) for carbohydrate transport. Bacterial cells comprising the Glu+ phenotype have modified endogenous chromosomal regulatory regions which are operably linked to polynucleotides encoding galactose permeases and glucokinases.
Abstract translation: 公开了一种将Glu +表型恢复到最初能够利用磷酸转移酶转运系统（PTS）用于碳水化合物转运的PTS- / Glu-细菌细胞的方法。 包含Glu +表型的细菌细胞具有修饰的内源性染色体调节区，其可操作地连接到编码半乳糖通透酶和葡糖激酶的多核苷酸。
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公开(公告)号：US20120288907A1
公开(公告)日：2012-11-15
申请号：US13471339
申请日：2012-05-14
Applicant: Marguerite A. Cervin , Philippe Soucaille , Fernando Valle , Gregory M. Whited
Inventor： Marguerite A. Cervin , Philippe Soucaille , Fernando Valle , Gregory M. Whited
Abstract: A method is disclosed for restoring a Glu+ phenotype to a PTS−/Glu− bacterial cell which was originally capable of utilizing a phosphotransferase transport system (PTS) for carbohydrate transport. Bacterial cells comprising the Glu+ phenotype have modified endogenous chromosomal regulatory regions which are operably linked to polynucleotides encoding galactose permeases and glucokinases.
Abstract translation: 公开了一种将Glu +表型恢复到最初能够利用磷酸转移酶转运系统（PTS）用于碳水化合物转运的PTS- / Glu-细菌细胞的方法。 包含Glu +表型的细菌细胞具有修饰的内源性染色体调节区，其可操作地连接到编码半乳糖通透酶和葡糖激酶的多核苷酸。
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