公开(公告)号：US08609363B2

公开(公告)日：2013-12-17

申请号：US13298682

申请日：2011-11-17

Applicant: Xin Heng ,  Eugene Breniman

Inventor： Xin Heng ,  Eugene Breniman

IPC: C12Q1/02 ,  C12Q1/00

CPC classification number: C12Q1/02 ,  G01N2015/1486

Abstract: Cell counts that distinguish between live and dead cells while providing an accurate count of the total of live and dead cells are obtained by the use of a vital stain in conjunction with illumination of the cell population and the taking of light images at different wavelengths, one which is not absorbed by the stain and one that is absorbed by the stain. Masking and inaccuracies in the counting of dead cells is thereby avoided.

Abstract translation: 通过使用生命染色结合细胞群的照射和摄取不同波长的光图像获得活细胞和死细胞之间的细胞计数，同时提供活细胞和死细胞的总数的精确计数。 其不被污渍吸收并被污渍所吸收。 从而避免了死细胞计数中的掩蔽和不准确。

公开(公告)号：US08411282B2

公开(公告)日：2013-04-02

申请号：US13157245

申请日：2011-06-09

Applicant: Xiquan Cui ,  Changhuei Yang ,  Axel Scherer ,  Demetri Psaltis ,  Xin Heng

Inventor： Xiquan Cui ,  Changhuei Yang ,  Axel Scherer ,  Demetri Psaltis ,  Xin Heng

IPC: G01B9/02

CPC classification number: G02B21/0056 ,  G01N21/553 ,  G02B21/18 ,  G02B21/361

Abstract: A differential interference contrast (DIC) determination device and method utilizes an illumination source, a layer having a pair of two apertures that receive illumination from the illumination source, and a photodetector to receive Young's interference from the illumination passing through the pair of two apertures. In addition, a surface wave assisted optofluidic microscope and method utilize an illumination source, a fluid channel having a layer with at least one aperture as a surface, and a photodetector that receives a signal based on the illumination passing through the aperture. The layer is corrugated (e.g., via fabrication) and parameters of the corrugation optimize the signal received on the photodetector.

Abstract translation: 差分干涉对比度（DIC）确定装置和方法利用照明源，具有一对两个孔的层，其接收来自照明源的照明，以及光电检测器，用于接收来自穿过该对两个孔的照明的杨氏干扰。 此外，表面波辅助光流体显微镜和方法利用照明源，具有至少一个孔的层作为表面的流体通道，以及基于穿过孔的照明而接收信号的光电检测器。 该层是波纹状的（例如通过制造），并且波纹的参数优化了在光电检测器上接收的信号。

公开(公告)号：US08189204B2

公开(公告)日：2012-05-29

申请号：US12792059

申请日：2010-06-02

Applicant: Xiquan Cui ,  Xin Heng ,  Changhuei Yang ,  Axel Scherer ,  Demetri Psaltis ,  Guoan Zheng

Inventor： Xiquan Cui ,  Xin Heng ,  Changhuei Yang ,  Axel Scherer ,  Demetri Psaltis ,  Guoan Zheng

IPC: G01B9/02

CPC classification number: G02B21/0056 ,  B82Y20/00 ,  G01N21/554 ,  G02B6/1226 ,  G02B21/10 ,  G02B21/14 ,  G02B27/52

Abstract: Embodiments of the present invention relate to a surface wave enabled darkfield aperture structure comprising an aperture layer, a aperture in the aperture layer and a plurality of grooves around the aperture. The aperture layer has a first and second surface. The plurality of grooves is in the first surface. A surface wave propagates along at least the first surface. The plurality of grooves is configured to generate a darkfield at the aperture by modifying the surface wave to cancel out direct transmission of a uniform incident light field received by the aperture.

Abstract translation: 本发明的实施例涉及一种表面波使能暗场孔结构，其包括孔径层，孔径层中的孔和围绕孔的多个凹槽。 孔层具有第一和第二表面。 多个槽位于第一表面。 表面波沿着至少第一表面传播。 多个槽被配置为通过修改表面波来在孔处产生暗场，以消除由孔接收的均匀入射光场的直接透射。

公开(公告)号：US20110075254A1

公开(公告)日：2011-03-31

申请号：US12792059

申请日：2010-06-02

Applicant: Xiquan Cui ,  Xin Heng ,  Changhuei Yang ,  Axel Scherer ,  Demetri Psaltis ,  Guoan Zheng

Inventor： Xiquan Cui ,  Xin Heng ,  Changhuei Yang ,  Axel Scherer ,  Demetri Psaltis ,  Guoan Zheng

IPC: G02B21/10 ,  G02B21/14

CPC classification number: G02B21/0056 ,  B82Y20/00 ,  G01N21/554 ,  G02B6/1226 ,  G02B21/10 ,  G02B21/14 ,  G02B27/52

Abstract: Embodiments of the present invention relate to a surface wave enabled darkfield aperture structure comprising an aperture layer, a aperture in the aperture layer and a plurality of grooves around the aperture. The aperture layer has a first and second surface. The plurality of grooves is in the first surface. A surface wave propagates along at least the first surface. The plurality of grooves is configured to generate a darkfield at the aperture by modifying the surface wave to cancel out direct transmission of a uniform incident light field received by the aperture.

Abstract translation: 本发明的实施例涉及一种表面波使能暗场孔结构，其包括孔径层，孔径层中的孔和围绕孔的多个凹槽。 孔层具有第一和第二表面。 多个槽位于第一表面。 表面波沿着至少第一表面传播。 多个槽被配置为通过修改表面波来在孔处产生暗场，以消除由孔接收的均匀入射光场的直接透射。

公开(公告)号：US08761486B2

公开(公告)日：2014-06-24

申请号：US13399808

申请日：2012-02-17

Applicant: Xin Heng ,  Paul Patt

Inventor： Xin Heng ,  Paul Patt

IPC: G06K9/00

CPC classification number: G01N15/1459 ,  G01N15/1434 ,  G01N2015/144

Abstract: Systems and methods for performing cytometry using a linear light sensor. An illumination field, a line scanned by the linear light sensor, or both are swept across a cell to be imaged. Relative motion between the cell and the swept illumination may be created using a movable optical component or components, by adhering cells to a plate and transporting the plate or by other techniques.

Abstract translation: 使用线性光传感器进行细胞计数的系统和方法。 照明场，由线性光传感器扫描的线或两者都扫过要成像的细胞。 电池和扫描照明之间的相对运动可以通过使用可移动的光学部件或组件，通过将电池附着到板上并传送该板或通过其它技术来产生。

公开(公告)号：US08570370B2

公开(公告)日：2013-10-29

申请号：US12869979

申请日：2010-08-27

Applicant: Tom McCollum ,  Paul Patt ,  Frank Shen ,  Daniel Y. Chu ,  Don Flory ,  Mike Griffin ,  Xin Heng ,  Eli Hefner

Inventor： Tom McCollum ,  Paul Patt ,  Frank Shen ,  Daniel Y. Chu ,  Don Flory ,  Mike Griffin ,  Xin Heng ,  Eli Hefner

IPC: G06K9/00

CPC classification number: C12Q1/06 ,  G01N15/1434 ,  G02B21/0008 ,  G02B21/34

Abstract: Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000,000 pixels each having an area of 2×2 μm or less and that images a field of view of at least 3 mm2. The sensor enables the counter to compress the optical components into an optical path of less than 20 cm in height when arranged vertically with no changes in direction of the optical path as a whole, and the entire instrument has a footprint of less than 300 cm2. Activation of the light source, automated focusing of the sensor image, and digital cell counting are all initiated by the simple insertion of the sample holder into the instrument. The suspension is placed in a sample chamber in the form of a slide that is shaped to ensure proper orientation of the slide in the cell counter.

Abstract translation: 将液体悬浮液中的生物细胞计数在自动细胞计数器中，其将悬浮液的图像聚焦在数字成像传感器上，该数字成像传感器包含至少4,000,000个像素，每个具有2×2μm或更小的面积，并且图像的视场 至少3 mm2。 该传感器使得计数器能够将光学部件压缩成高度小于20cm的光路，当垂直布置时，整个光路的方向没有变化，并且整个仪器的占地面积小于300cm 2。 光源的激活，传感器图像的自动聚焦和数字单元计数都是通过将样品架简单插入到仪器中来启动的。 将悬浮液置于样品室中，其形状为成形以确保细胞计数器中载玻片的正确取向。

公开(公告)号：US20120211679A1

公开(公告)日：2012-08-23

申请号：US13212459

申请日：2011-08-18

Applicant: Xin Heng ,  Paul Patt

Inventor： Xin Heng ,  Paul Patt

IPC: G01N21/85 ,  G01N21/64

CPC classification number: G01N15/1434 ,  G01N15/1436 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/645 ,  G01N2021/6421

Abstract: A flow cytometry system includes a flow element through which a cell is transported in a flowing fluid. The flow element includes a bore bounded by a wall. A light source is configured to illuminate the cell. An optical system receives light emanating from the cell and directs at least some of the received light to a light sensor. The optical system includes a numerical-aperture-increasing lens at a wall of the flow element. At least some of the received light passes through the numerical-aperture-increasing lens. The flow cytometry system may also include a beam splitter that directs two wavelength bands of the emanating light such that light in two wavelength band preferentially reach different sensing locations via different paths. The system may also include an optical element placed in one of the paths, shifting a focal location of the affected path to compensate for chromatic aberration of the numerical-aperture-increasing lens.

Abstract translation: 流式细胞术系统包括流动元件，细胞在流动元件中以流动的流体输送。 流动元件包括由壁限定的孔。 光源被配置为照亮单元。 光学系统接收从单元发出的光并且将至少一些所接收的光引导到光传感器。 光学系统在流动元件的壁处包括数字孔径增加透镜。 所接收的光中的至少一些通过数字孔径增加透镜。 流式细胞术系统还可以包括分束器，其引导发射光的两个波长带，使得两个波长带中的光优先通过不同的路径到达不同的感测位置。 该系统还可以包括放置在路径之一中的光学元件，移动受影响的路径的焦点位置以补偿数值孔径增加透镜的色差。

公开(公告)号：US20110063623A1

公开(公告)日：2011-03-17

申请号：US12823201

申请日：2010-06-25

Applicant: Xiquan Cui ,  Xin Heng ,  Changhuei Yang ,  Axel Scherer ,  Demetri Psaltis

Inventor： Xiquan Cui ,  Xin Heng ,  Changhuei Yang ,  Axel Scherer ,  Demetri Psaltis

IPC: G01B9/02

CPC classification number: G02B21/0056 ,  G01N21/553 ,  G02B21/18 ,  G02B21/361

Abstract: A differential interference contrast (DIC) determination device and method utilizes an illumination source, a layer having a pair of two apertures that receive illumination from the illumination source, and a photodetector to receive Young's interference from the illumination passing through the pair of two apertures. In addition, a surface wave assisted optofluidic microscope and method utilize an illumination source, a fluid channel having a layer with at least one aperture as a surface, and a photodetector that receives a signal based on the illumination passing through the aperture. The layer is corrugated (e.g., via fabrication) and parameters of the corrugation optimize the signal received on the photodetector.

Abstract translation: 差分干涉对比度（DIC）确定装置和方法利用照明源，具有一对两个孔的层，其接收来自照明源的照明，以及光电检测器，用于接收来自穿过该对两个孔的照明的杨氏干扰。 此外，表面波辅助光流体显微镜和方法利用照明源，具有至少一个孔的层作为表面的流体通道，以及基于穿过孔的照明而接收信号的光电检测器。 该层是波纹状的（例如通过制造），并且波纹的参数优化了在光电检测器上接收的信号。

公开(公告)号：US07768654B2

公开(公告)日：2010-08-03

申请号：US11743581

申请日：2007-05-02

Applicant: Xiquan Cui ,  Xin Heng ,  Changhuei Yang ,  Axel Scherer ,  Demetri Psaltis

Inventor： Xiquan Cui ,  Xin Heng ,  Changhuei Yang ,  Axel Scherer ,  Demetri Psaltis

IPC: G01B9/02

CPC classification number: G02B21/0056 ,  G01N21/553 ,  G02B21/18 ,  G02B21/361

Abstract: A differential interference contrast (DIC) determination device and method utilizes an illumination source, a layer having a pair of two apertures that receive illumination from the illumination source, and a photodetector to receive Young's interference from the illumination passing through the pair of two apertures. In addition, a surface plasmon assisted optofluidic microscope and method utilize an illumination source, a fluid channel having a layer with at least one aperture as a surface, and a photodetector that receives a signal based on the illumination passing through the aperture. The layer is corrugated (e.g., via fabrication) and parameters of the corrugation optimize the signal received on the photodetector.

Abstract translation: 差分干涉对比度（DIC）确定装置和方法利用照明源，具有一对两个孔的层，其接收来自照明源的照明，以及光电检测器，用于接收来自穿过该对两个孔的照明的杨氏干扰。 此外，表面等离子体激元辅助光流体显微镜和方法利用照明源，具有至少一个孔的层作为表面的流体通道，以及基于穿过孔的照明而接收信号的光电检测器。 该层是波纹状的（例如通过制造），并且波纹的参数优化了在光电检测器上接收的信号。

公开(公告)号：US20060061769A1

公开(公告)日：2006-03-23

申请号：US11218918

申请日：2005-09-02

Applicant: Changhuei Yang ,  Xin Heng ,  Zahid Yaqoob

Inventor： Changhuei Yang ,  Xin Heng ,  Zahid Yaqoob

IPC: G01B9/02

CPC classification number: G02B21/0056 ,  G01B9/04

Abstract: Optical coherence microscope (OCM) systems and methods that combine the capability of a confocal microscope to obtain high resolution images and the ability of low coherence interferometer (LCI) to obtain high-accuracy phase and amplitude information of samples. The OCM system of the present invention uses a homodyne approach and obtains complete quadrature results of amplitude and phase instantaneously without optical or electronic modulation as in conventional OCT systems. Because the OCM methods of the present invention use a homodyne approach for signal extraction, there is no minimum pixel dwell time associated with each pixel acquisition, and accurate interference phase and amplitude information is extracted.

Abstract translation: 光学相干显微镜（OCM）系统和方法结合共焦显微镜的能力获得高分辨率图像和低相干干涉仪（LCI）获得样品的高精度相位和幅度信息的能力。 本发明的OCM系统使用零差分方法，并且如在常规OCT系统中那样，无需光或电调制即可获得幅度和相位的完全正交结果。 由于本发明的OCM方法使用零差分方法进行信号提取，因此不存在与每个像素采集相关联的最小像素停留时间，并且提取精确的干扰相位和振幅信息。