公开(公告)号：US20150031026A1

公开(公告)日：2015-01-29

申请号：US14360892

申请日：2012-11-28

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Stephen Hendricks ,  David King ,  Lei Xi ,  Marian Peris

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12Q1/46 ,  C12P19/34 ,  C12Q1/6834 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

Abstract translation: 在一些实施方案中，用于连接核酸末端的方法包括：在存在可产生可连接的末端5'磷酸基团的至少一种试剂的存在下进行核酸连接反应，通过从核酸的末端5'磷酸脱除腺苷酸基 。 在一些实施方案中，阿伐他汀酶可以催化从核酸的末端5'磷酸脱除腺苷酸基团。 在一些实施方案中，用于连接核酸末端的方法包括：在适于连接核酸末端的条件下，在阿伐他汀酶存在下进行核酸连接反应。

公开(公告)号：US11072824B2

公开(公告)日：2021-07-27

申请号：US16656839

申请日：2019-10-18

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shiaw-Min Chen ,  Elana Swartzman ,  David Ruff ,  Mark Shannon ,  Julia Lu ,  Stephen Hendricks

IPC: C12Q1/68 ,  C12Q1/6855 ,  C12Q1/6804

Abstract: This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.

公开(公告)号：US10472671B2

公开(公告)日：2019-11-12

申请号：US14955386

申请日：2015-12-01

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Shiaw-Min Chen ,  Elana Swartzman ,  David Ruff ,  Mark Shannon ,  Julia Lu ,  Stephen Hendricks

IPC: C12Q1/68 ,  C12Q1/6855 ,  C12Q1/6804

Abstract: This application relates to methods for ligating oligonucleotides having complementarity to a target nucleic acid, and amplifying the ligated oligonucleotides, where ligation and amplification occur in the same reaction mixture.

公开(公告)号：US09399767B2

公开(公告)日：2016-07-26

申请号：US14191997

申请日：2014-02-27

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Marian Peris ,  Michael Phelan ,  Barnett Rosenblum ,  Stephen Hendricks

IPC: C12N9/12 ,  C12Q1/68

CPC classification number: C12N9/1252 ,  C12Q1/68 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6869 ,  C12Q1/6872 ,  C12Y207/07007 ,  G01N2500/00 ,  C12Q2565/632 ,  C12Q2563/137 ,  C12Q2563/107 ,  C12Q2565/30 ,  C12Q2537/157

Abstract: Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.

Abstract translation: 本文提供了衍生自野生型RB69 DNA聚合酶或与野生型RB69 DNA聚合酶相关的突变型DNA依赖性聚合酶。 这些突变型聚合酶能够选择性地结合标记的核苷酸。 这些突变型聚合酶还能够掺入多种天然存在和修饰的核苷酸，包括例如终止子核苷酸。

公开(公告)号：US20150191775A1

公开(公告)日：2015-07-09

申请号：US14593858

申请日：2015-01-09

Applicant: Life Technologies Corporation

Inventor： Lei XI ,  Paul Kenney ,  Zhaochun Ma ,  Dennis Prosen ,  Stephen Hendricks ,  Roland Nagel

IPC: C12Q1/68

CPC classification number: C12Q1/686 ,  C07H21/00 ,  C07H21/02 ,  C12N9/1252 ,  C12N15/10 ,  C12Q1/6869 ,  C12Y207/07007

Abstract: This disclosure relates to methods of performing activation by polyphosphorolysis (APP) reactions using at least one of the polyphosphorylating agents triphosphate, polyphosphate, imidodiphosphate, thiodiphosphate (or μ-monothiopyrophosphate), and related compounds.

Abstract translation: 本公开涉及使用多磷酸盐，多磷酸盐，亚氨基二磷酸盐，硫代磷酸盐（或μ-一硫代焦磷酸盐）和相关化合物中的至少一种进行多磷酸解（APP）反应进行活化的方法。

公开(公告)号：US10597705B2

公开(公告)日：2020-03-24

申请号：US16154529

申请日：2018-10-08

Applicant: LIFE TECHNOLOGIES CORPORATION

Inventor： Stephen Hendricks ,  David King ,  Lei Xi ,  Marian Peris

IPC: C12Q1/68 ,  C12Q1/6853 ,  C12Q1/6844 ,  C12Q1/6806 ,  C12P19/34 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q1/6834 ,  C12Q1/46

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.

公开(公告)号：US20160186224A1

公开(公告)日：2016-06-30

申请号：US15071086

申请日：2016-03-15

Applicant: Life Technologies Corporation

Inventor： Stephen Hendricks ,  David King ,  Lei Xi ,  Marian Peris

IPC: C12P19/34

CPC classification number: C12Q1/46 ,  C12P19/34 ,  C12Q1/6834 ,  C12Q1/6846 ,  C12Q1/6853 ,  C12Q1/6869 ,  C12Q1/6874 ,  C12Q2531/119 ,  C12Q2565/537

Abstract: In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of at least one agent that generates a ligatable terminal 5′ phosphate group by removing an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, an aprataxin enzyme can catalyze removal of an adenylate group from a terminal 5′ phosphate of a nucleic acid. In some embodiments, methods for ligating nucleic acid ends comprise: conducting a nucleic acid ligation reaction in the presence of an aprataxin enzyme under conditions suitable for ligating nucleic acid ends.