公开(公告)号：US20190258052A1

公开(公告)日：2019-08-22

申请号：US16322157

申请日：2017-08-02

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Florian FAHRBACH ,  Werner KNEBEL

IPC: G02B27/00 ,  G02B21/00

Abstract: A light sheet microscope or a confocal microscope includes illumination optics configured to transmit light of at least two wavelengths from at least one light source, along respective wavelength-dependent beam paths, from an illumination side of the illumination optics to a specimen side of the illumination optics. A lateral chromatic correction system comprising at least one optical lateral chromatic correction element is configured such that the beam paths of the at least two different wavelengths have, at a specimen-side output of the lateral chromatic correction element, an offset parallel to each other and/or are inclined relative to each other in relation to the illumination side, which, on the specimen side of the illumination optics, results in an offset of the foci of the at least two wavelengths transverse to an optical axis of the illumination optics.

公开(公告)号：US20180149851A1

公开(公告)日：2018-05-31

申请号：US15566728

申请日：2016-04-18

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Werner KNEBEL ,  Frank SIECKMANN ,  Florian FAHRBACH

IPC: G02B21/18 ,  G02B21/36 ,  G02B21/00 ,  G01N21/47

CPC classification number: G02B21/18 ,  G01N21/47 ,  G02B21/0052 ,  G02B21/0064 ,  G02B21/06 ,  G02B21/10 ,  G02B21/362 ,  G02B21/367

Abstract: A method for single plane illumination microscopy (SPIM) analysis of a sample includes simultaneously illuminating multiple sample layers by a single sheet of light. Detection light emanating from the individual sample layers is detected at different times and/or at different positions in a detection beam path. The detection beam path is branched using beam splitters and an effective refractive power of the individual beam splitters is zero.

公开(公告)号：US20170160531A1

公开(公告)日：2017-06-08

申请号：US15327391

申请日：2015-07-22

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Werner KNEBEL

IPC: G02B21/16 ,  G01N21/64 ,  G02B21/00 ,  G02B21/06 ,  G02B27/56

CPC classification number: G02B21/16 ,  G01N21/6458 ,  G01N21/648 ,  G02B21/002 ,  G02B21/06 ,  G02B27/56

Abstract: A method for microscopic examination of a specimen includes bringing the specimen into contact with an optically transparent medium that has a higher refractive index than the specimen. An illumination light bundle is generated and directed through an illumination objective that focuses the illumination light bundle. The illumination light bundle that has passed through the illumination objective in the direction of the specimen that is to be examined is deflected using a deflector arranged on a detection objective in such a way that the illumination light bundle strikes a boundary surface between the optically transparent medium and the specimen, where the illumination light bundle is totally reflected for purposes of evanescently illuminating the specimen. Fluorescent light that is emitted by the specimen and that passes through the detection objective is detected.

公开(公告)号：US20160153892A1

公开(公告)日：2016-06-02

申请号：US14899157

申请日：2014-06-18

Applicant: Leica Microsystems CMS GmbH

Inventor： Werner KNEBEL ,  Wernher FOUQUET ,  Frank SIECKMANN

IPC: G01N21/13 ,  G02B21/26 ,  G02B21/00 ,  G02B21/06

CPC classification number: G01N21/13 ,  G01N2021/135 ,  G02B21/0024 ,  G02B21/0032 ,  G02B21/0076 ,  G02B21/06 ,  G02B21/26 ,  G02B21/34 ,  G02B21/367

Abstract: The invention relates to a method for microscopic investigation of a plurality of samples. The method contains the step of arranging the samples in a sample holder that is movable, in particular in motorized and/or automatic fashion, relative to a sample illumination position in such a way that at least one of the samples is respectively successively positionable in the sample illumination position, a clearance for a deflection means respectively remaining adjacent to the sample that is currently located in the sample illumination position; the step of focusing a light stripe with an illumination objective; the step of deflecting the light stripe, once it has passed through the illumination objective, with the deflection means in such a way that the light stripe propagates at an angle different from zero degrees with respect to the optical axis of the illumination objective and has a focus in the sample illumination position; and the step of successively positioning the samples, retained with the sample holder, in the sample illumination position, and detecting the detected light emerging from the sample respectively located in the sample illumination position. The invention furthermore relates to an optical apparatus having a sample holder that holds a plurality of samples and is supported movably, in particular in motorized and/or automatic fashion, relative to a sample illumination position in such a way that at least one of the samples is respectively successively positionable in the sample illumination position.

Abstract translation: 本发明涉及一种用于多个样品的微观研究的方法。 该方法包括将样品布置在样品保持器中，该样品保持器相对于样品照射位置，特别是以电动和/或自动的方式是可移动的，使得至少一个样品分别可连续定位在 样本照明位置，分别保持与当前位于样本照明位置的样本相邻的偏转装置的间隙; 将光条与照明物体对焦的步骤; 一旦透过照明物镜，偏转装置以使光条以相对于照明物镜的光轴为零度的角度传播的方式偏转光条的步骤，并且具有 聚焦在样品照明位置; 以及将样本保持器保留的样本连续定位在样本照射位置的步骤，以及检测从分别位于样本照明位置的样品出射的检测到的光。 本发明还涉及一种光学装置，其具有保持多个样品的样品保持器，并且以相对于样品照射位置可移动地尤其以机动和/或自动的方式被支撑，使得至少一个样品 分别连续定位在样本照明位置。