公开(公告)号：US20220373778A1

公开(公告)日：2022-11-24

申请号：US17265531

申请日：2019-07-19

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Florian FAHRBACH

IPC: G02B21/08

Abstract: A illumination arrangement for a microscope for illuminating a sample with a light sheet includes an illumination input configured to feed an illumination beam along an optical axis of the illumination arrangement and an illumination output which faces a sample side and is configured to output the illumination beam to the sample side. A focusing optical system is provided with a set depth of focus. At least one optical modification element is configured to geometrically modify the illumination beam. Different rays of the illumination beam intersect the optical axis within an axis intersection region at the illumination output. The axis intersection region extends over at least the depth of focus of the focusing optical system along the optical axis.

公开(公告)号：US20220342196A1

公开(公告)日：2022-10-27

申请号：US17636050

申请日：2020-08-31

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Florian FAHRBACH

IPC: G02B21/36 ,  G02B21/00

Abstract: A control device for controlling a microscope includes: a computation module. The control device: adjusts a sample volume velocity of a sample volume of the microscope relative to the microscope; and adjusts a light sheet velocity of the light sheet of the microscope relative to the microscope based on the sample volume velocity.

公开(公告)号：US20210278651A1

公开(公告)日：2021-09-09

申请号：US17292474

申请日：2019-11-12

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Werner KNEBEL ,  Florian FAHRBACH ,  Christof FRANKE ,  Dirk-Oliver FEHRER

IPC: G02B21/08 ,  G02B21/00 ,  G02B21/04 ,  G02B21/24

Abstract: A light sheet microscope includes: a detection objective configured to image a target region of a sample located in a focal plane of the detection objective; an illumination objective configured to focus an illumination light beam in the sample, the detection objective and the illumination objective being opposite to one another, and the optical axis of the detection objective and the optical axis of the illumination objective being parallel to one another; a first light deflection device having at least one first deflection surface and one second deflection surface, which are each arranged offset to the optical axis of the detection objective and are configured to deflect the illumination light beam focused by the illumination objective in a direction perpendicular to the optical axis of the detection objective such that a deflected illumination light beam forms a light-sheet-like illumination light distribution focused in the focal plane.

公开(公告)号：US20190025563A1

公开(公告)日：2019-01-24

申请号：US15766375

申请日：2016-10-10

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Florian FAHRBACH ,  Werner KNEBEL

IPC: G02B21/00 ,  G02B21/18 ,  G02B21/06

Abstract: A method for examining a sample in light sheet fluorescence microscopy includes generating an illumination light beam using a light source. The illumination light beam is spatially split into at least two partial illumination light beams using a splitter. The partial illumination light beams are guided through an illumination objective shared by the partial illumination light beams. After the partial illumination light beams have passed through the illumination objective, at least one of the partial illumination light beams is deflected using at least one deflector such that the partial illumination light beams interfere with one another in an illumination plane so as to generate an illumination pattern in the illumination plane. An image of a sample region illuminated by the illumination pattern is produced, wherein detection light that emanates from the sample region reaches a position-sensitive detector through a detection objective.

公开(公告)号：US20210063714A1

公开(公告)日：2021-03-04

申请号：US16977820

申请日：2019-02-20

Applicant: Leica Microsystems CMS GmbH

Inventor： Florian FAHRBACH ,  Werner KNEBEL ,  Peter POTT ,  Wernher FOUQUET

IPC: G02B21/00 ,  G02B21/33 ,  G02B21/24 ,  G01N21/64 ,  G01N21/47

Abstract: An optical device for illuminating a sample located in a sample volume with illumination light and for detecting scattered and/or fluorescent light from the sample includes an optical illumination assembly, an optical detection assembly and at least one attachment element. The optical illumination assembly is configured to transmit the illumination light along an illumination path into the sample volume. The optical detection assembly is configured to collect and relay the scattered and/or fluorescent light from the sample volume along a detection path. At least portions of the illumination path and/or of the detection path extend in the at least one attachment element.

公开(公告)号：US20190391375A1

公开(公告)日：2019-12-26

申请号：US16462242

申请日：2017-11-10

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Florian FAHRBACH

IPC: G02B21/00

Abstract: An illumination arrangement for a microscope includes an illumination input configured to inject an illumination beam bundle and an illumination output configured to output at least two partial beam bundles generated from the illumination beam bundle. At least one diffractive optical element is configured to split the illumination beam bundle into the at least two partial beam bundles that propagate along partial beam paths, and is configured to effect a relative change in respective propagation directions of the at least two partial beam bundles with respect to one another, such that the at least two partial beam bundles output by the illumination arrangement are non-collinear with respect to one another at the illumination output.

公开(公告)号：US20190278072A1

公开(公告)日：2019-09-12

申请号：US16310472

申请日：2017-06-21

Applicant: Leica Microsystems CMS GmbH

Inventor： Florian FAHRBACH ,  Lars FRIEDRICH ,  Werner KNEBEL

IPC: G02B21/16 ,  G02B21/06 ,  G02B27/10

Abstract: An illumination apparatus for a microscope, for producing a de-excitation or switching light distribution, includes a light source configured to produce a primary illumination light beam and a beam splitter configured to divide the primary illumination light beam into two partial illumination light beams. An illumination objective is configured to focus the partial illumination light beams onto and/or into a sample such that the partial illumination light beams extend, spatially separated from one another, through an entry pupil of the illumination objective and are spatially superposed on and/or in the sample after passing through the illumination objective. A phase influencer is configured to cause a relative phase offset of the partial illumination light beams with respect to one another in such a way that the partial illumination light beams in the entry pupil of the illumination objective have a phase offset of π.

公开(公告)号：US20190258052A1

公开(公告)日：2019-08-22

申请号：US16322157

申请日：2017-08-02

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Florian FAHRBACH ,  Werner KNEBEL

IPC: G02B27/00 ,  G02B21/00

Abstract: A light sheet microscope or a confocal microscope includes illumination optics configured to transmit light of at least two wavelengths from at least one light source, along respective wavelength-dependent beam paths, from an illumination side of the illumination optics to a specimen side of the illumination optics. A lateral chromatic correction system comprising at least one optical lateral chromatic correction element is configured such that the beam paths of the at least two different wavelengths have, at a specimen-side output of the lateral chromatic correction element, an offset parallel to each other and/or are inclined relative to each other in relation to the illumination side, which, on the specimen side of the illumination optics, results in an offset of the foci of the at least two wavelengths transverse to an optical axis of the illumination optics.

公开(公告)号：US20190204580A1

公开(公告)日：2019-07-04

申请号：US16325215

申请日：2017-08-16

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Florian FAHRBACH

IPC: G02B21/36 ,  G02B21/00 ,  G01N21/64

CPC classification number: G02B21/367 ,  G01N21/6458 ,  G02B21/0032 ,  G02B21/0076

Abstract: A light sheet microscope includes an illuminator having a beam source which is designed to direct an illuminating beam propagating along an illumination axis onto a sample. A light-sheet generator is designed to generate a light-sheet-type illuminating light distribution illuminating the sample in one partial region. A detection unit has a detector that is designed to capture detection light originating from the section of the sample that is illuminated by the illuminating light distribution. An objective is provided for both the illuminator and the detection unit such that the objective is to be penetrated by the illuminating beam and the detection light. The illuminator has a beam modulator designed to modulate the illuminating beam perpendicular to the illumination axis.

公开(公告)号：US20180149851A1

公开(公告)日：2018-05-31

申请号：US15566728

申请日：2016-04-18

Applicant: LEICA MICROSYSTEMS CMS GMBH

Inventor： Werner KNEBEL ,  Frank SIECKMANN ,  Florian FAHRBACH

IPC: G02B21/18 ,  G02B21/36 ,  G02B21/00 ,  G01N21/47

CPC classification number: G02B21/18 ,  G01N21/47 ,  G02B21/0052 ,  G02B21/0064 ,  G02B21/06 ,  G02B21/10 ,  G02B21/362 ,  G02B21/367

Abstract: A method for single plane illumination microscopy (SPIM) analysis of a sample includes simultaneously illuminating multiple sample layers by a single sheet of light. Detection light emanating from the individual sample layers is detected at different times and/or at different positions in a detection beam path. The detection beam path is branched using beam splitters and an effective refractive power of the individual beam splitters is zero.