公开(公告)号：US20240132954A1

公开(公告)日：2024-04-25

申请号：US18400702

申请日：2023-12-29

Applicant: ILLUMINA, INC.

Inventor： Timothy J. Merkel ,  Wayne N. George ,  Andrew A. Brown ,  Audrey Zak ,  Gianluca Andrea Artioli ,  Julia Morrison ,  Nikolai Romanov ,  Lorenzo Berti ,  Graham Boud

IPC: C12Q1/6874 ,  C08G77/442 ,  C08L33/04 ,  C08L63/00 ,  C09D153/00 ,  C09D163/00 ,  C12Q1/6876

CPC classification number: C12Q1/6874 ,  C08G77/442 ,  C08L33/04 ,  C08L63/00 ,  C09D153/00 ,  C09D163/00 ,  C12Q1/6876

Abstract: An example of a resin composition includes a free radical curable resin matrix including an acrylate and a siloxane, and a free radical photoinitiator. When cured, the resin composition has low or no autofluorescence when exposed to blue excitation wavelengths ranging from about 380 nm to about 480 nm or green excitation wavelengths ranging from about 510 nm to about 560 nm.

公开(公告)号：US20230391997A1

公开(公告)日：2023-12-07

申请号：US18233276

申请日：2023-08-11

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Wayne N. George ,  Ludovic Vincent ,  Andrew A. Brown ,  Mathieu Lessard-Viger

IPC: C08L33/08 ,  C12Q1/6869 ,  C12Q1/6853 ,  C08L33/26

CPC classification number: C08L33/08 ,  C08L33/26 ,  C12Q1/6853 ,  C12Q1/6869

Abstract: A flow cell includes a support and a heteropolymer attached to the support. The heteropolymer includes an acrylamide monomer including an attachment group to react with a functional group attached to a primer, and a monomer including a stimuli-responsive functional group. The monomer including the stimuli-responsive functional group may be pH-responsive, temperature-responsive, saccharide-responsive, nucleophile-responsive, and/or salt-responsive.

公开(公告)号：US11680292B2

公开(公告)日：2023-06-20

申请号：US17840569

申请日：2022-06-14

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16 ,  G03F7/00 ,  G03F7/004

CPC classification number: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/0757 ,  G03F7/165 ,  G03F7/0002 ,  G03F7/0046

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20230016633A1

公开(公告)日：2023-01-19

申请号：US17838986

申请日：2022-06-13

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Xiaoyu Ma ,  Weixian Xi ,  Jeffrey S. Fisher ,  Fei Shen ,  Wayne N. George ,  Brian D. Mather

IPC: C08K5/54 ,  C12Q1/6874 ,  B01J19/00

Abstract: Embodiments of the present application relate to substrate comprising a surface-bound azido functionalized organosilane wherein the substrate is free or substantially free of a hydrogel or a hydrophilic polymer. Methods of preparing such substrate surface for sequencing applications are also disclosed.

公开(公告)号：US20230002822A1

公开(公告)日：2023-01-05

申请号：US17840569

申请日：2022-06-14

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US11512339B2

公开(公告)日：2022-11-29

申请号：US15848640

申请日：2017-12-20

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Wayne N. George ,  Alexandre Richez ,  M. Shane Bowen ,  Andrew A. Brown ,  Dajun Yuan ,  Audrey Rose Zak ,  Sean M. Ramirez ,  Raymond Campos

IPC: C09D183/06 ,  C12Q1/68 ,  C09D133/26 ,  C08G77/14

Abstract: An example of an array includes a support, a cross-linked epoxy polyhedral oligomeric silsesquioxane (POSS) resin film on a surface of the support, and a patterned hydrophobic polymer layer on the cross-linked epoxy POSS resin film. The patterned hydrophobic polymer layer defines exposed discrete areas of the cross-linked epoxy POSS resin film, and a polymer coating is attached to the exposed discrete areas. Another example of an array includes a support, a modified epoxy POSS resin film on a surface of the support, and a patterned hydrophobic polymer layer on the modified epoxy POSS resin film. The modified epoxy POSS resin film includes a polymer growth initiation site, and the patterned hydrophobic polymer layer defines exposed discrete areas of the modified epoxy POSS resin film. A polymer brush is attached to the polymer growth initiation site in the exposed discrete areas.

公开(公告)号：US11213976B2

公开(公告)日：2022-01-04

申请号：US15847150

申请日：2017-12-19

Applicant: Illumina, Inc. ,  Illumina Cambridge Limited

Inventor： Alexandre Richez ,  Andrew A. Brown ,  Julia Morrison ,  Wayne N. George ,  Timothy J. Merkel ,  Audrey Rose Zak

IPC: B29C33/38 ,  C09D133/26 ,  B29C59/02 ,  G03F7/00 ,  B29C33/40 ,  C09D4/00 ,  B29L31/00

Abstract: An imprinting apparatus includes a silicon master having a plurality of nanofeatures defined therein. An anti-stick layer coats the silicon master, the anti-stick layer including a molecule having a cyclosiloxane with at least one silane functional group. A method includes forming a master template by: depositing a formulation on a silicon master including a plurality of nanofeatures defined therein, the formulation including a solvent and a molecule having a cyclosiloxane with at least one silane functional group; and curing the formulation, thereby forming an anti-stick layer on the silicon master, the anti-stick layer including the molecule. The method further includes depositing a silicon-based working stamp material on the anti-stick layer of the master template; curing the silicon-based working stamp material to form a working stamp including a negative replica of the plurality of nanofeatures; and releasing the working stamp from the master template.

公开(公告)号：US11124829B2

公开(公告)日：2021-09-21

申请号：US16626452

申请日：2019-06-07

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown ,  Dajun Yuan

IPC: C12Q1/68 ,  G03F7/16 ,  C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/00 ,  G03F7/004

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US20210139975A1

公开(公告)日：2021-05-13

申请号：US17139834

申请日：2020-12-31

Applicant: ILLUMINA, INC. ,  ILLUMINA CAMBRIDGE LIMITED

Inventor： Jeffrey S. Fisher ,  Brian D. Mather ,  Maria Candelaria Rogert Bacigalupo ,  Justin Fullerton ,  Ludovic Vincent ,  Lewis J. Kraft ,  Sahngki Hong ,  Boyan Boyanov ,  M. Shane Bowen ,  Sang Park ,  Wayne N. George ,  Andrew A. Brown ,  Dajun Yuan

IPC: C12Q1/6869 ,  C08F220/56 ,  C08G77/04 ,  G03F7/075 ,  G03F7/16

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

公开(公告)号：US09994687B2

公开(公告)日：2018-06-12

申请号：US14316478

申请日：2014-06-26

Applicant: Illumina, Inc.

Inventor： Lorenzo Berti ,  Andrew A. Brown ,  Wayne N. George

IPC: C08J7/12 ,  C12Q1/68 ,  C08F8/00 ,  C09D133/26 ,  B01J19/00 ,  C08F220/60

CPC classification number: C08J7/12 ,  B01J19/0046 ,  B01J2219/00529 ,  B01J2219/00533 ,  B01J2219/00619 ,  B01J2219/00626 ,  B01J2219/00637 ,  B01J2219/00722 ,  C08F8/00 ,  C08F2220/603 ,  C08J2335/00 ,  C09D133/26 ,  C12Q1/6837 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q2527/125 ,  C12Q2565/537 ,  C12Q2565/629

Abstract: Some embodiments described herein relate to a substrate with a surface comprising a silane or a silane derivative covalently attached to optionally substituted cycloalkene or optionally substituted heterocycloalkene for direct conjugation with a functionalized molecule of interest, such as a polymer, a hydrogel, an amino acid, a nucleoside, a nucleotide, a peptide, a polynucleotide, or a protein. In some embodiments, the silane or silane derivative contains optionally substituted norbornene or norbornene derivatives. Method for preparing a functionalized surface and the use in DNA sequencing and other diagnostic applications are also disclosed.