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公开(公告)号：US20210025757A1
公开(公告)日：2021-01-28
申请号：US16604557
申请日：2018-04-12
Applicant: CAPTL LLC
Inventor： Masanobu Yamamoto , Keegan Hernandez , J. Paul Robinson
Abstract: A measurement system includes an optical source (e.g., laser) to irradiate a sample (e.g., a cell); a solid-state photon detector (SSPD) to receive resultant light from the sample; and a photon counter to count photons received by the SSPD. The photon counter can include a differentiator to provide a differentiated photon signal and a crossing detector configured to count photons based on a number of times the differentiated photon signal crosses a predetermined threshold level. In some examples, a pulse detector can provide a pulse-width signal from the SSPD output photon signal, and a pulse counter can count based on both a number of pulses and widths of the pulses. The SSPD can include a silicon photomultiplier (SiPM) array or a solid-state photomultiplier. Some examples use the measurement system to measure samples in fluids, e.g., in flow cytometers or multi-well plates.
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公开(公告)号：US10775292B2
公开(公告)日：2020-09-15
申请号：US16045094
申请日：2018-07-25
Applicant: CAPTL LLC
Inventor： Masanobu Yamamoto , J. Paul Robinson
Abstract: An image flow cytometer for observing a microparticulate sample includes a flow chamber having a flow channel that permits the microparticulate sample to travel in a flow direction. An irradiation system scans an irradiation spot across a sensing area of the flow channel in a scan direction different from the flow direction. A detection system detects resultant light from the sensing area and provides a detection signal. An alignment system alters a location of the sensing area with respect to the flow chamber. A control unit causes the irradiation system to scan the irradiation spot during a first measurement interval and operates the alignment system to translate the location of the sensing area along the flow direction. The flow chamber can be mounted to a movable stage in some examples, and the alignment system can move the flow chamber substantially opposite the flow direction using the stage.
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公开(公告)号：US10060850B2
公开(公告)日：2018-08-28
申请号：US15564117
申请日：2016-04-01
Applicant: CAPTL LLC
Inventor： Masanobu Yamamoto , J. Paul Robinson
CPC classification number: G01N21/47 , B01L3/502715 , B01L2300/0654 , B01L2300/0806 , B01L2300/168 , B01L2400/0406 , B01L2400/0409 , B01L2400/049 , G01N15/1425 , G01N15/1429 , G01N15/1434 , G01N15/147 , G01N15/1484 , G01N21/4788 , G01N21/55 , G01N21/645 , G01N2015/1006 , G01N2021/6439 , G01N2021/6463
Abstract: An example assembly includes a target holder that retains a target in a detection region. A reflective surface reflects at least part of a focused spot of light to provide resultant light. An irradiation system irradiates at least part of the detection region with the focused spot of light. A motion system causes motion of the focused spot of light relative to the reflective surface. A detection system detects the resultant light. An example device, e.g., a lab-on-chip, includes a substrate, a sample inlet, and a reflective grating. The grating is retains a fluidic sample in a detection region fluidically connected to the sample inlet. The detection region is operatively arranged with respect to the reflective grating so that at least a portion of light passing through the detection region towards the reflective grating also passes through the detection region after reflecting off the reflective grating.
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Patent Agency Ranking
公开(公告)号：US20220146401A1
公开(公告)日：2022-05-12
申请号：US17583062
申请日：2022-01-24
Applicant: CAPTL LLC
Inventor： Masanobu Yamamoto , J. Paul Robinson , Keegan Hernandez
IPC: G01N15/14
Abstract: A system is described wherein multiple lasers are used to irradiate particles in a flow cytometer's flow stream. In certain embodiments, a light source having a first laser configured for continuous irradiation of a flow stream and one or more second lasers configured for irradiation of the flow stream in discrete intervals where each discrete interval of irradiation by the second laser is triggered by irradiation of one or more particles in the flow stream with the first laser. Methods for modulating the laser irradiation and measuring light intensity are also described. Also described is the computations and systems required to operate the one or more second lasers.
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公开(公告)号：US20220082440A1
公开(公告)日：2022-03-17
申请号：US17536068
申请日：2021-11-28
Applicant: CAPTL LLC
Inventor： Masanobu Yamamoto , Keegan Hernandez , J. Paul Robinson
Abstract: A measurement system includes an optical source (e.g., laser) to irradiate a sample (e.g., a cell); a solid-state photon detector (SSPD) to receive resultant light from the sample; and a photon counter to count photons received by the SSPD. The photon counter can include a differentiator to provide a differentiated photon signal and a crossing detector configured to count photons based on a number of times the differentiated photon signal crosses a predetermined threshold level. In some examples, a pulse detector can provide a pulse-width signal from the SSPD output photon signal, and a pulse counter can count based on both a number of pulses and widths of the pulses. The SSPD can include a silicon photomultiplier (SiPM) array or a solid-state photomultiplier. Some examples use the measurement system to measure samples in fluids, e.g., in flow cytometers or multi-well plates.
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公开(公告)号：US10036698B2
公开(公告)日：2018-07-31
申请号：US15187346
申请日：2016-06-20
Applicant: CAPTL LLC
Inventor： Masanobu Yamamoto , J. Paul Robinson
CPC classification number: G01N15/1427 , G01N15/1404 , G01N15/1434 , G01N15/147 , G01N15/1475 , G01N2015/1006 , G01N2015/1409 , G01N2015/1413 , G01N2015/144 , G01N2015/1452
Abstract: An image flow cytometer for observing a microparticulate sample includes a flow chamber having a flow channel that permits the microparticulate sample to travel in a flow direction. An irradiation system scans an irradiation spot across a sensing area of the flow channel in a scan direction different from the flow direction. A detection system detects resultant light from the sensing area and provides a detection signal. An alignment system alters a location of the sensing area with respect to the flow chamber. A control unit causes the irradiation system to scan the irradiation spot during a first measurement interval and operates the alignment system to translate the location of the sensing area along the flow direction. The flow chamber can be mounted to a movable stage in some examples, and the alignment system can move the flow chamber substantially opposite the flow direction using the stage.
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公开(公告)号：US20180080870A1
公开(公告)日：2018-03-22
申请号：US15564117
申请日：2016-04-01
Applicant: CAPTL LLC
Inventor： Masanobu Yamamoto , J. Paul Robinson
CPC classification number: G01N21/47 , B01L3/502715 , B01L2300/0654 , B01L2300/0806 , B01L2300/168 , B01L2400/0406 , B01L2400/0409 , B01L2400/049 , G01N15/1425 , G01N15/1429 , G01N15/1434 , G01N15/147 , G01N15/1484 , G01N21/4788 , G01N21/55 , G01N21/645 , G01N2015/1006 , G01N2021/6439 , G01N2021/6463
Abstract: An example assembly includes a target holder that retains a target in a detection region. A reflective surface reflects at least part of a focused spot of light to provide resultant light. An irradiation system irradiates at least part of the detection region with the focused spot of light. A motion system causes motion of the focused spot of light relative to the reflective surface. A detection system detects the resultant light. An example device, e.g., a lab-on-chip, includes a substrate, a sample inlet, and a reflective grating. The grating is retains a fluidic sample in a detection region fluidically connected to the sample inlet. The detection region is operatively arranged with respect to the reflective grating so that at least a portion of light passing through the detection region towards the reflective grating also passes through the detection region after reflecting off the reflective grating.
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公开(公告)号：US20170059485A1
公开(公告)日：2017-03-02
申请号：US15250136
申请日：2016-08-29
Applicant: CAPTL LLC
Inventor： Masanobu Yamamoto , J. Paul Robinson
CPC classification number: G01N33/582 , B01L3/502761 , G01N15/1434 , G01N15/1456 , G01N15/1459 , G01N15/1484 , G01N21/645 , G01N35/00069 , G01N2035/00881 , G01N2201/1045
Abstract: A measurement system includes a system for causing relative motion between a sample and an irradiation spot. The sample includes fluorescent markers having respective wavelengths. A gating system provides a gating signal based at least in part on resultant light substantially at an irradiation wavelength. A detection system detects fluorescent light from the irradiated markers and provides detection signals representing the fluorescent light detected concurrently with a gate-open signal. In some examples, the detection system detects fluorescent light at multiple wavelengths and provides respective detection signals. A spectral discriminator arranged optically between the sample and the detection system receives the fluorescent light from the sample and provides respective fluorescent light at the wavelengths to the detection system. A flow cytometer can spectrally disperse resultant fluorescent light and measure the wavelengths separately. Light from a sample disposed over a reflective phase grating can be dispersed, measured, and gated.
Abstract translation: 测量系统包括用于引起样品和照射点之间的相对运动的系统。 样品包括具有各自波长的荧光标记。 门控系统至少部分地提供基本上在照射波长处的合成光的门控信号。 检测系统检测来自照射标记的荧光，并提供表示与门开启信号同时检测的荧光的检测信号。 在一些示例中，检测系统检测多个波长的荧光并提供相应的检测信号。 在样本和检测系统之间光学地布置的光谱鉴别器接收来自样品的荧光，并向检测系统提供波长的相应荧光。 流式细胞仪可以光谱分散所得的荧光并分别测量波长。 来自设置在反射相位光栅上的样品的光可以被分散，测量和门控。
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