公开(公告)号：US11187584B2

公开(公告)日：2021-11-30

申请号：US16604557

申请日：2018-04-12

Applicant: CAPTL LLC

Inventor： Masanobu Yamamoto ,  Keegan Hernandez ,  J. Paul Robinson

IPC: G01J3/44 ,  G01J3/02

Abstract: A measurement system includes an optical source (e.g., laser) to irradiate a sample (e.g., a cell); a solid-state photon detector (SSPD) to receive resultant light from the sample; and a photon counter to count photons received by the SSPD. The photon counter can include a differentiator to provide a differentiated photon signal and a crossing detector configured to count photons based on a number of times the differentiated photon signal crosses a predetermined threshold level. In some examples, a pulse detector can provide a pulse-width signal from the SSPD output photon signal, and a pulse counter can count based on both a number of pulses and widths of the pulses. The SSPD can include a silicon photomultiplier (SiPM) array or a solid-state photomultiplier. Some examples use the measurement system to measure samples in fluids, e.g., in flow cytometers or multi-well plates.

公开(公告)号：US20220146401A1

公开(公告)日：2022-05-12

申请号：US17583062

申请日：2022-01-24

Applicant: CAPTL LLC

Inventor： Masanobu Yamamoto ,  J. Paul Robinson ,  Keegan Hernandez

IPC: G01N15/14

Abstract: A system is described wherein multiple lasers are used to irradiate particles in a flow cytometer's flow stream. In certain embodiments, a light source having a first laser configured for continuous irradiation of a flow stream and one or more second lasers configured for irradiation of the flow stream in discrete intervals where each discrete interval of irradiation by the second laser is triggered by irradiation of one or more particles in the flow stream with the first laser. Methods for modulating the laser irradiation and measuring light intensity are also described. Also described is the computations and systems required to operate the one or more second lasers.

公开(公告)号：US20220082440A1

公开(公告)日：2022-03-17

申请号：US17536068

申请日：2021-11-28

Applicant: CAPTL LLC

Inventor： Masanobu Yamamoto ,  Keegan Hernandez ,  J. Paul Robinson

IPC: G01J3/44 ,  G01J3/02

Abstract: A measurement system includes an optical source (e.g., laser) to irradiate a sample (e.g., a cell); a solid-state photon detector (SSPD) to receive resultant light from the sample; and a photon counter to count photons received by the SSPD. The photon counter can include a differentiator to provide a differentiated photon signal and a crossing detector configured to count photons based on a number of times the differentiated photon signal crosses a predetermined threshold level. In some examples, a pulse detector can provide a pulse-width signal from the SSPD output photon signal, and a pulse counter can count based on both a number of pulses and widths of the pulses. The SSPD can include a silicon photomultiplier (SiPM) array or a solid-state photomultiplier. Some examples use the measurement system to measure samples in fluids, e.g., in flow cytometers or multi-well plates.

公开(公告)号：US20220057317A1

公开(公告)日：2022-02-24

申请号：US17415311

申请日：2019-12-16

Applicant: CAPTL LLC

Inventor： Masanobu Yamamoto ,  J. Paul Robinson ,  Keegan Hernandez

IPC: G01N15/14 ,  H01L31/02 ,  H01L31/107

Abstract: An example system can include a support and two or more sensor elements mounted to the support. Each sensor element can be electrically connected to a common electrical node and may include: a respective quench resistor connected to a respective internal node; and a respective photodiode (PD) connected to the respective internal node; a differentiating element fed by at least one of the photodiodes; a first readout electrode fed by the common electrical node; and a second readout electrode fed by the differentiating element. The common electrical node may be connected to at least one of the quench resistors or at least one of the photodiodes.

公开(公告)号：US11255771B2

公开(公告)日：2022-02-22

申请号：US16908638

申请日：2020-06-22

Applicant: CAPTL LLC

Inventor： Masanobu Yamamoto ,  J. Paul Robinson ,  Keegan Hernandez

IPC: G01N15/14

Abstract: A photon-accounting system for use with a flow cytometry system is disclosed which includes a signal shaping sub-system, including a differentiator configured to generate a differentiated output of photodiode signals into corresponding zero-crossings each associated with one of the received photons, a comparator configured to receive the differentiated signal and compare to a threshold to thereby generate a comparator output digital signal associated with the crossing of the differentiated signal about the threshold, a front-end synchronization system adapted to receive and synchronize the comparator generated digital signal to a clock, thereby generate synchronized photon data with the clock and associated with the asynchronized photodiode signal, and a timestamping system adapted to receive the synchronized data as a bit stream and generate a timestamp associated with each photon data.

公开(公告)号：US20210025757A1

公开(公告)日：2021-01-28

申请号：US16604557

申请日：2018-04-12

Applicant: CAPTL LLC

Inventor： Masanobu Yamamoto ,  Keegan Hernandez ,  J. Paul Robinson

IPC: G01J3/44 ,  G01J3/02

Abstract: A measurement system includes an optical source (e.g., laser) to irradiate a sample (e.g., a cell); a solid-state photon detector (SSPD) to receive resultant light from the sample; and a photon counter to count photons received by the SSPD. The photon counter can include a differentiator to provide a differentiated photon signal and a crossing detector configured to count photons based on a number of times the differentiated photon signal crosses a predetermined threshold level. In some examples, a pulse detector can provide a pulse-width signal from the SSPD output photon signal, and a pulse counter can count based on both a number of pulses and widths of the pulses. The SSPD can include a silicon photomultiplier (SiPM) array or a solid-state photomultiplier. Some examples use the measurement system to measure samples in fluids, e.g., in flow cytometers or multi-well plates.