公开(公告)号：US09099436B2

公开(公告)日：2015-08-04

申请号：US13852476

申请日：2013-03-28

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Aditya Rajagopal ,  Axel Scherer ,  Michael D. Henry ,  Sameer Walavalkar ,  Thomas A. Tombrello ,  Andrew P. Homyk

IPC: G01R1/067 ,  H01L29/423 ,  H01L29/78 ,  H01L29/66 ,  G01N27/327 ,  G01N27/414 ,  A61B5/04 ,  H01L29/41 ,  H01L29/49 ,  G01R1/073

CPC classification number: C12Q1/02 ,  A61B5/04001 ,  G01N27/3275 ,  G01N27/4146 ,  G01R1/06711 ,  G01R1/07342 ,  H01L29/413 ,  H01L29/42376 ,  H01L29/4966 ,  H01L29/66477 ,  H01L29/78 ,  H01L29/7831

Abstract: The basic structure and functionality of a probe as disclosed herein allows for flexibly incorporating into the probe, various sensing elements for various sensing applications. Two example applications among these various sensing applications include bio-sensing and chemical-sensing applications. For bio-sensing applications the probe, which is fabricated upon a silicon substrate, includes a bio-sensing element such as a nano-pillar transistor, and for chemical-sensing applications the probe includes a sensing element that has a functionalized contact area whereby the sensing element generates a voltage when exposed to one or more chemicals of interest.

Abstract translation: 本文公开的探针的基本结构和功能允许灵活地并入到探针中，用于各种感测应用的各种感测元件。 这些各种感测应用中的两个示例应用包括生物感测和化学感测应用。 对于生物感测应用，制造在硅衬底上的探针包括诸如纳米柱晶体管的生物感测元件，并且对于化学感测应用，探针包括具有功能化接触区域的感测元件， 当暴露于感兴趣的一种或多种化学品时，感测元件产生电压。

公开(公告)号：US20130327645A1

公开(公告)日：2013-12-12

申请号：US13967108

申请日：2013-08-14

Applicant: California Institute of Technology

Inventor： Sameer Walavalkar ,  Axel Scherer ,  Thomas A. Tombrello ,  Aditya Rajagopal ,  Andrew P. Homyk ,  Erika Garcia

IPC: G01N27/447

CPC classification number: G01N27/447 ,  B82Y30/00 ,  C12Q1/6869 ,  G01N33/48721 ,  C12Q2563/116 ,  C12Q2565/631

Abstract: Methods and devices for sequencing nucleic acids are disclosed herein. Devices are also provided herein for measuring DNA with nano-pores sized to allow DNA to pass through the nano-pore. The capacitance can be measured for the DNA molecule passing through the nano-pore. The capacitance measurements can be correlated to determine the sequence of base pairs passing through the nano-pore to sequence the DNA.

Abstract translation: 本文公开了用于测序核酸的方法和装置。 本文还提供了用于测量具有大小允许DNA穿过纳米孔的纳米孔的DNA的装置。 可以测量通过纳米孔的DNA分子的电容。 电容测量可以相关，以确定通过纳米孔的碱基对序列来对DNA进行排序。

公开(公告)号：US10081844B2

公开(公告)日：2018-09-25

申请号：US15675048

申请日：2017-08-11

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12P19/34 ,  C12Q1/70 ,  C12Q1/6851 ,  C12Q1/68

CPC classification number: C12Q1/701 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/703 ,  Y10T436/143333 ,  C12Q2561/113 ,  C12Q2565/101 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2565/133

Abstract: Methods of detecting at least one genetic variation in a polynucleotide analyte in a sample. A fluorophore is attached to a first primer, a quencher is attached to a second primer, the first primer and the second primer are specific for the polynucleotide analyte. A signal generated by the fluorophore and quencher is measured. PCR is performed with the first primer and the second primer using the polynucleotide analyte as a template, thereby amplifying the template. A signal generated by the fluorophore and quencher from the PCR amplification product is measured. Comparison is made of the signals; and a determination is made of the presence or absence of the at least one genetic variation based i) on the change in signal as determined; and ii) by comparing said change to the change in signal observed upon PCR amplification for a corresponding polynucleotide analyte lacking the at least one genetic variation.

公开(公告)号：US20180030551A1

公开(公告)日：2018-02-01

申请号：US15675048

申请日：2017-08-11

Applicant: California Institute of Technology

Inventor： Aditya Rajagopal ,  Mark D. Goldberg ,  Erika F. Garcia ,  Xiomara L. Madero ,  Thomas A. Tombrello ,  Axel Scherer

IPC: C12Q1/70 ,  C12Q1/68

CPC classification number: C12Q1/701 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/703 ,  Y10T436/143333 ,  C12Q2561/113 ,  C12Q2565/101 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2565/133

Abstract: This disclosure provides methods, compositions and kits for the detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, compositions, and kits for detecting analytes, genetic variations, monitoring reaction process, and monitoring analyte-analyte interactions by measuring signals. In some examples, the presence of signals or changes in signals may be used to construct signal profiles which can be used to detect analytes.