公开(公告)号：US20140100102A1

公开(公告)日：2014-04-10

申请号：US14048735

申请日：2013-10-08

Applicant: California Institute Of Technology

Inventor： Aditya RAJAGOPAL ,  Emil P. KARTALOV ,  Carl J. ALLENDORPH ,  Axel SCHERER ,  Samson CHEN

IPC: C12M1/00

CPC classification number: C12M47/06

Abstract: Methods and devices for molecular analysis are disclosed, based on centrifugation. A centrifuge device comprises strips of centrifuge tubes and elements to create a magnetic field. The magnetic shear forces applied to beads inside a solution with biological molecules permit the performance of different analytic techniques, such as lysis and sample preparation for PCR.

Abstract translation: 公开了基于离心的分子分析方法和装置。 离心机装置包括离心管和元件条，以产生磁场。 施加到具有生物分子的溶液中的珠的磁剪切力允许执行不同的分析技术，例如用于PCR的裂解和样品制备。

公开(公告)号：US20130149714A1

公开(公告)日：2013-06-13

申请号：US13706668

申请日：2012-12-06

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  Sanofi Aventis

Inventor： Axel SCHERER ,  Remi BROUARD, III ,  Emil P. KARTALOV ,  Jingqing HUANG

IPC: A61B5/1459 ,  A61B5/145 ,  A61B5/01 ,  A61B5/20 ,  A61B5/1491 ,  A61B3/10 ,  A61B5/00

CPC classification number: A61B5/1459 ,  A61B3/10 ,  A61B5/0002 ,  A61B5/01 ,  A61B5/14507 ,  A61B5/1451 ,  A61B5/14546 ,  A61B5/1491 ,  A61B5/20 ,  A61B5/418 ,  A61B5/42 ,  A61B5/4337 ,  A61B5/6848 ,  A61B5/6852 ,  A61B5/6866 ,  A61B5/7278 ,  A61B2560/0219 ,  A61B2560/0233

Abstract: An implantable diagnostic device in accordance with the present disclosure includes a probe assembly that can be implemented in a variety of ways. A few example implementations include: a needle inside which is located a bio-sensor chip (the needle being insertable into a human being); a compact package containing the bio-sensor chip (the compact package configured for placement inside a catheter); or a silicon-based bio-sensor package configured for insertion into a vein.

Abstract translation: 根据本公开的可植入诊断装置包括可以以各种方式实现的探针组件。 一些示例性实现包括：位于其内的针，其位于生物传感器芯片（针可插入人体中）; 包含生物传感器芯片的紧凑型封装（被配置用于放置在导管内的紧凑封装）; 或被配置为插入静脉中的硅基生物传感器封装。

公开(公告)号：US20240018573A1

公开(公告)日：2024-01-18

申请号：US18352112

申请日：2023-07-13

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. KARTALOV ,  Aditya RAJAGOPAL ,  Axel SCHERER

IPC: C12Q1/6825 ,  G16B25/00 ,  G16B5/00 ,  C12Q1/6851 ,  G16B25/20 ,  G16B40/10 ,  C12Q1/68 ,  G01N21/64

CPC classification number: C12Q1/6825 ,  G16B25/00 ,  G16B5/00 ,  C12Q1/6851 ,  G16B25/20 ,  G16B40/10 ,  C12Q1/68 ,  G01N21/6486 ,  G06F17/10

Abstract: This disclosure provides methods, systems, compositions, and kits for the multiplexed detection of a plurality of analytes in a sample. In some examples, this disclosure provides methods, systems, compositions, and kits wherein multiple analytes may be detected in a single sample volume by acquiring a cumulative measurement or measurements of at least one quantifiable component of a signal. In some cases, additional components of a signal, or additional signals (or components thereof) are also quantified. Each signal or component of a signal may be used to construct a coding scheme which can then be used to determine the presence or absence of any analyte.

公开(公告)号：US20220289348A1

公开(公告)日：2022-09-15

申请号：US17582991

申请日：2022-01-24

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. KARTALOV ,  Axel SCHERER

IPC: B63C11/02 ,  G01N27/22 ,  G01N21/41

Abstract: Methods and devices including implantable micro-sensors used to detect tissue-dissolved inert gas and to detect microbubble formation to avoid Caisson disease are described. The disclosed methods and devices are based on measuring the refractive index changes in hydrophobic liquids after absorbing an inert gas such as nitrogen. The changes in the refractive index are based on implementing one of an interferometry, optical microcavity resonance shift, a photonic crystal resonance, a beam deflection, a resonance tuning or detuning, an amplitude change, or an intensity change method.

公开(公告)号：US20180171330A1

公开(公告)日：2018-06-21

申请号：US15885352

申请日：2018-01-31

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  UNIVERSITY OF SOUTHERN CALIFORNIA ,  CURATE BIOSCIENCES LLC

Inventor： Emil P. KARTALOV ,  Cheng-Chung LEE ,  Paul PREDKI

IPC: C12N15/10 ,  C12Q1/6874 ,  C12Q1/686 ,  B01L3/00

CPC classification number: C12N15/1093 ,  B01L3/502707 ,  B01L2300/0816 ,  B01L2300/0887 ,  C12Q1/6853 ,  C12Q1/686 ,  C12Q1/6874 ,  C12Q2563/179

Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation device described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation device can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation device can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation device can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device. Also included are methods for selectively isolating cellular material using the devices described herein, as are methods for biochemical analysis of individual regions of interest of cellular material using the devices described herein. Further included are methods of making masking arrays useful for the methods described herein. The micro-isolation devices can comprise a unique combination of barcodes in each microfluidics well, allowing two-dimensional mapping of genetic information.

公开(公告)号：US20150322395A1

公开(公告)日：2015-11-12

申请号：US14802384

申请日：2015-07-17

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY ,  UNIVERSITY OF SOUTHERN CALIFORNIA

Inventor： Emil P. KARTALOV ,  Darryl SHIBATA ,  Clive TAYLOR ,  Lawrence A. WADE

IPC: C12M3/06 ,  C12M1/12 ,  C12M1/00

CPC classification number: C12M23/16 ,  C12M23/12 ,  C12M23/22 ,  C12M25/04 ,  C12M25/14 ,  C12M35/02 ,  C12M37/04 ,  C12M47/04 ,  C12N13/00 ,  G01N33/54366

Abstract: Provided herein are devices and methods for the micro-isolation of biological cellular material. A micro-isolation apparatus described can comprise a photomask that protects regions of interest against DNA-destroying illumination. The micro-isolation apparatus can further comprise photosensitive material defining access wells following illumination and subsequent developing of the photosensitive material. The micro-isolation apparatus can further comprise a chambered microfluidic device comprising channels providing access to wells defined in photosensitive material. The micro-isolation apparatus can comprise a chambered microfluidic device without access wells defined in photosensitive material where valves control the flow of gases or liquids through the channels of the microfluidic device. Also included are methods for selectively isolating cellular material using the apparatuses described herein, as are methods for biochemical analysis of individual regions of interest of cellular material using the devices described herein. Further included are methods of making masking arrays useful for the methods described herein.

公开(公告)号：US20150167092A1

公开(公告)日：2015-06-18

申请号：US14633094

申请日：2015-02-26

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： Emil P. KARTALOV ,  Aditya RAJAGOPAL ,  Axel SCHERER ,  Mark D. GOLDBERG

IPC: C12Q1/68

CPC classification number: C12Q1/6883 ,  C12Q1/68 ,  C12Q1/6851 ,  C12Q1/701 ,  C12Q1/703 ,  C12Q2600/156 ,  Y10T436/143333 ,  C12Q2561/113 ,  C12Q2565/101 ,  C12Q2527/101 ,  C12Q2565/1015 ,  C12Q2565/133

Abstract: FRET-based analytes detection and related methods and systems are described where a pair of FRET labeled primers and/or oligonucleotides are used that are specific for target sequences located at a distance up to four time the Förster distance of the FRET chromophores presented on the FRET labeled primers and/or oligonucleotides one with respect to the other in one or more polynucleotide analyte; in particular the pair of FRET labeled primers and/or oligonucleotides is combined with a sample and subjected to one or more polynucleotide amplification reactions before measuring FRET signals from at least one FRET chromophore.

Abstract translation: 描述了基于FRET的分析物检测和相关方法和系统，其中使用一对FRET标记的引物和/或寡核苷酸，其特异于靶向序列，其位于与FRET上呈现的FRET发色团的Förster距离的四倍 在一个或多个多核苷酸分析物中相对于另一个标记的引物和/或寡核苷酸; 特别地，一对FRET标记的引物和/或寡核苷酸与样品组合，并在测量来自至少一种FRET生色团的FRET信号之前进行一个或多个多核苷酸扩增反应。