公开(公告)号：US20140057283A1

公开(公告)日：2014-02-27

申请号：US13802174

申请日：2013-03-13

Applicant: ACEA BIOSCIENCES, INC

Inventor： Xiaobo Wang ,  Xiao Xu

IPC: G01N27/02

CPC classification number: G01N27/02 ,  C12M23/12 ,  C12M41/36 ,  G01N27/3276 ,  Y10T29/49117

Abstract: A method for assaying target molecules in a sample liquid, the method comprising: providing an impedance monitoring device operably connected to an impedance analyzer; adding a sample liquid suspected of having target molecules to the well thereby permitting binding of target molecules to the capture molecules; monitoring impedance of the well; and determining the presence, amount or concentration of target molecules in the liquid sample from the monitored impedance. The device includes a nonconducting substrate having a well, at least two electrodes fabricated on a bottom of the well and on a same plane, wherein the surfaces of the at least two electrodes are modified with capture molecules configured to bind target molecules in a liquid sample, and at least two connection pads electrically connected to the at least two electrodes.

Abstract translation: 一种用于测定样品液体中的靶分子的方法，所述方法包括：提供可操作地连接到阻抗分析仪的阻抗监测装置; 将怀疑具有靶分子的样品液体加入到孔中，从而允许靶分子与捕获分子的结合; 监测井的阻抗; 以及从所监测的阻抗确定液体样品中靶分子的存在，量或浓度。 该器件包括具有阱的非导电衬底，至少两个电极，其制造在阱的底部并且在同一平面上，其中至少两个电极的表面被配置成结合在液体样品中的靶分子结合的俘获分子 以及至少两个电连接到所述至少两个电极的连接焊盘。

公开(公告)号：US20200158670A1

公开(公告)日：2020-05-21

申请号：US16721742

申请日：2019-12-19

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaobo Wang ,  Wei Ouyang ,  Nan Li ,  Tianxing Wang ,  Xiaoyu Zhang ,  Xiao Xu ,  Yama A. Abassi

IPC: G01N27/02 ,  G01N33/483

Abstract: Devices, systems and methods for monitoring excitable cells, such as cardiomyocytes, on microelectrode arrays that couple the electro-stimulation of excitable cells to induce or regulate cardiomyocyte beating and the simultaneous measurement of impedance and extracellular recording to assess changes in cardiomyocyte beating, viability, morphology or electrophysical properties in response to a plurality of treatments.

公开(公告)号：US20180306771A1

公开(公告)日：2018-10-25

申请号：US16020679

申请日：2018-06-27

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaobo Wang ,  Yama A. Abassi ,  Biao Xi ,  Wen Fu Zhang ,  Xiao Xu

IPC: G01N33/483

Abstract: A system for monitoring cell-substrate impedance of excitable cells and monitoring the beating cycle of cardiomyocytes, the system including: a device for monitoring cell-substrate impedance at 20 millisecond time resolution, the device having one or more wells on a nonconductive substrate, wherein at least one well of the one or more wells comprises an electrode array fabricated on the substrate for measurement of cell-substrate impedance at 20 millisecond time resolution; an impedance analyzer that measures cell-substrate impedance from the at least one well at 20 millisecond time resolution; electronic circuitry that electrically connects the electrode array from the at least one well to the impedance analyzer; and a software program that analyzes the measured cell-substrate impedance.

公开(公告)号：US20180246079A1

公开(公告)日：2018-08-30

申请号：US15523649

申请日：2016-11-21

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaobo Wang ,  Yama A Abassi ,  Nan Li ,  Lincoln Muir ,  Xiao Xu

IPC: G01N33/50 ,  C12N13/00 ,  C12M1/34 ,  G01N33/487

CPC classification number: G01N33/5011 ,  C12M1/3407 ,  C12N13/00 ,  G01N33/4836 ,  G01N33/48707 ,  G01N33/502 ,  G01N33/5047 ,  G01N2800/52 ,  G01N2800/7028

Abstract: Methods of assessing cytolysis of cancer cells, including providing a cell-substrate impedance monitoring device operably connected to an impedance analyzer, wherein the device comprises a well for receiving cells and an electrode array at a base of the well; adding target cells characterized as cancer cells to the well; adding effector cells to the well to form a test well, wherein the effector cells are immune cells obtained or derived from a same patient as the target cells; monitoring cell-substrate impedance of the test well before and after adding the effector cells and optionally deriving an impedance-based parameter from the impedance; and determining effectiveness of effector cell killing of the target cells by comparing the impedance or impedance based parameter over time.

公开(公告)号：US20180095064A1

公开(公告)日：2018-04-05

申请号：US15651882

申请日：2017-07-17

Applicant: ACEA Biosciences, Inc.

Inventor： Xiaobo Wang ,  Yama A. Abassi ,  Biao Xi ,  Wen Fu Zhang ,  Xiao Xu

IPC: G01N33/483

CPC classification number: G01N33/4836

Abstract: A system for monitoring impedance of excitable cells in vitro, which includes a device for monitoring cell-substrate impedance at 20 millisecond resolution, which includes a nonconductive substrate with one or more electrode arrays fabricated in one or more wells, wherein cell attachment on the substrate can result in a detectable change in impedance between electrodes within each electrode array; an impedance analyzer capable of impedance measurement at 20 millisecond time resolution; electronic circuitry that can engage said device and selectively connect said two or more electrode arrays of said device to said impedance analyzer; and a software program that controls said electronic circuitry and records and analyzes data obtained from said impedance analyzer.

公开(公告)号：US20170315131A1

公开(公告)日：2017-11-02

申请号：US15487349

申请日：2017-04-13

Applicant: ACEA Biosciences, Inc.

Inventor： Xiao Xu ,  Yama A. Abassi ,  Xiaobo Wang ,  Jiangbo Gan

IPC: G01N33/68 ,  C12M1/34 ,  C12M1/12 ,  G01N33/483 ,  G01N33/50

CPC classification number: G01N33/6854 ,  C12M25/08 ,  C12M41/36 ,  G01N33/4836 ,  G01N33/5005 ,  G01N2500/10

Abstract: Methods for identifying compounds that modulate cellular responses stimulated by IgE, which include providing an impedance-based system that monitors cell-substrate impedance of cells on a substrate; introducing cells to the substrate of the system; adding at least one test compound and IgE to the cells, wherein the at least one test compound is suspected of modulating cell responses stimulated by the IgE; adding an antigen to the cells; monitoring the cell-substrate impedance of cells on the substrate; and analyzing the cell-substrate impedance to evaluate whether the at least one test compound alters a cellular response to stimulation with the IgE.

公开(公告)号：US20170269062A1

公开(公告)日：2017-09-21

申请号：US15612688

申请日：2017-06-02

Applicant: ACEA Biosciences, Inc.

Inventor： Yama A. Abassi ,  Li Zhao ,  Ning Ke ,  Xiaobo Wang ,  Xiao Xu

IPC: G01N33/50 ,  G01N33/543 ,  C12Q1/00

CPC classification number: G01N33/5014 ,  C12Q1/002 ,  C40B30/04 ,  G01N33/5011 ,  G01N33/5041 ,  G01N33/5438 ,  G01N2333/4716

Abstract: Methods of identifying whether a biologically active agent affects an oncogene addicted pathway within a cancer cell, by introducing a biologically active agent suspected of affecting an oncogene addicted pathway to a first well and a negative control to a second well, and introducing a stimulating agent that stimulates the oncogene addicted pathway to both wells; monitoring cell-substrate impedance of the two wells and optionally determining cell indices from impedance values; generating an impedance based curve for each of the two wells from the impedance values or from the cell indices; comparing the impedance-based curves to determine a degree of similarity; and if significantly different concluding the biologically active agent affects the oncogene addicted pathway within the cancer cells.

公开(公告)号：US09763949B2

公开(公告)日：2017-09-19

申请号：US14712794

申请日：2015-05-14

Applicant: ACEA BIOSCIENCES INC.

Inventor： Xiao Xu ,  Xiaobo Wang ,  Long Mao ,  Li Zhao ,  Biao Xi

IPC: A01N43/90 ,  A61K31/519 ,  C07D487/00 ,  A61K45/06 ,  C07D487/04

CPC classification number: A61K31/519 ,  A61K45/06 ,  C07D487/04 ,  A61K2300/00

Abstract: The present invention relates to certain pyrrolopyrimidine derivatives, pharmaceutical compositions containing them, and methods of using them, including methods for the treatment of tumors and related diseases related to the dysregulation of kinase (such as EGFR (including HER), Alk, PDGFR, but not limited to) pathways.

公开(公告)号：US09399787B2

公开(公告)日：2016-07-26

申请号：US13720124

申请日：2012-12-19

Applicant: ACEA BIOSCIENCES, INC

Inventor： Yama A. Abassi ,  Xiaobo Wang ,  Xiao Xu

IPC: G01R27/08 ,  C12Q1/00 ,  C12Q1/70 ,  C12Q1/02 ,  G01N27/02 ,  G01N33/50 ,  G01N33/543 ,  G01N33/483

CPC classification number: C12Q1/025 ,  C12Q1/002 ,  G01N27/02 ,  G01N33/4836 ,  G01N33/5014 ,  G01N33/5438

Abstract: A method of performing an assay of a response of two or more cell types to a test compound, including: providing a device for monitoring cell-substrate impedance, adding at least two different cell types to the device; adding at least one test compound to form at least two test compound wells; providing at least two control wells; monitoring impedance of the at least two test compound wells and of the at least two control wells at three or more time points after adding the at least one test compound; and analyzing measured impedance from the at least two test compound wells and from the at least two control wells at the three or more time points to obtain information on the response of the different cell types to the at least one test compound.

Abstract translation: 一种对测试化合物进行两种或多种细胞类型的反应测定的方法，包括：提供用于监测细胞 - 基底阻抗的装置，向装置添加至少两种不同的细胞类型; 加入至少一种测试化合物以形成至少两个测试化合物孔; 提供至少两个对照孔; 在加入至少一种测试化合物之后，在三个或更多个时间点监测所述至少两个测试化合物孔和所述至少两个对照孔的阻抗; 以及在所述三个或更多个时间点分析来自所述至少两个测试化合物孔和所述至少两个对照孔的测量阻抗，以获得关于所述不同细胞类型对所述至少一种测试化合物的响应的信息。

公开(公告)号：US20150185206A1

公开(公告)日：2015-07-02

申请号：US14541024

申请日：2014-11-13

Applicant: ACEA Biosciences, Inc.

Inventor： Yama A. Abassi ,  Naichen Yu ,  Josephine Atienza ,  Xiao Xu ,  Xiaobo Wang

IPC: G01N33/50 ,  G01N27/02

CPC classification number: G01N33/5041 ,  C12Q1/002 ,  G01N27/021 ,  G01N33/5014 ,  G01N33/5438 ,  G01N2333/4716 ,  G01N2333/705 ,  G01N2333/91205 ,  G01N2500/04 ,  G01N2500/10

Abstract: Use of cell-substrate impedance based methods for screening for agonists of G-Protein Coupled Receptors (GPCRs) or inhibitors of a Receptor Tyrosine Kinases (RTKs), identifying compounds that affect GPCR or RTK pathways, validating molecular targets involved in a GPCR or RTK signaling pathways, monitoring dose-dependent functional activation of GPCR or RTK; determining desensitization of a GPCR and identifying a compound capable of affecting RTK activity in cancer cell proliferation.

Abstract translation: 使用基于细胞底物阻抗的方法筛选G-蛋白偶联受体（GPCR）的激动剂或受体酪氨酸激酶（RTK）的抑制剂，鉴定影响GPCR或RTK途径的化合物，验证参与GPCR或RTK的分子靶标 信号通路，监测GPCR或RTK的剂量依赖性功能活化; 测定GPCR的脱敏作用并鉴定能够影响癌细胞增殖中的RTK活性的化合物。