公开(公告)号：US20170269062A1

公开(公告)日：2017-09-21

申请号：US15612688

申请日：2017-06-02

Applicant: ACEA Biosciences, Inc.

Inventor： Yama A. Abassi ,  Li Zhao ,  Ning Ke ,  Xiaobo Wang ,  Xiao Xu

IPC: G01N33/50 ,  G01N33/543 ,  C12Q1/00

CPC classification number: G01N33/5014 ,  C12Q1/002 ,  C40B30/04 ,  G01N33/5011 ,  G01N33/5041 ,  G01N33/5438 ,  G01N2333/4716

Abstract: Methods of identifying whether a biologically active agent affects an oncogene addicted pathway within a cancer cell, by introducing a biologically active agent suspected of affecting an oncogene addicted pathway to a first well and a negative control to a second well, and introducing a stimulating agent that stimulates the oncogene addicted pathway to both wells; monitoring cell-substrate impedance of the two wells and optionally determining cell indices from impedance values; generating an impedance based curve for each of the two wells from the impedance values or from the cell indices; comparing the impedance-based curves to determine a degree of similarity; and if significantly different concluding the biologically active agent affects the oncogene addicted pathway within the cancer cells.